Role of GTP-CHⅠ links PAH and TH in melanin synthesis in silkworm, Bombyx mori
详细信息 查看官网全文
- 英文论文题名：Role of GTP-CHⅠ links PAH and TH in melanin synthesis in silkworm, Bombyx mori
- 论文作者：陈萍 ; Jiying Wang ; Haiyin Li ; Yan Li ; Peng Chen ; Tian Li ; Xi Chen ; Junjie Xiao ; Liang Zhang
- 英文论文作者：Ping Chen ; Jiying Wang ; Haiyin Li ; Yan Li ; Peng Chen ; Tian Li ; Xi Chen ; Junjie Xiao ; Liang Zhang ; College of Biotechnology ; Southwest University
- 年：2016
- 作者机构：College of Biotechnology, Southwest University;
- 英文论文关键词：silkworm ; guanosine triphosphate cyclohydrolase Ⅰ(GTP-CH Ⅰ) ; phenylalanine hydroxylase(PAH) ; tyrosine hydroxylase(TH) ; melanin synthesis
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S881.2
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：570k
- 原文格式：D
- 会议级别：全国
摘要
In insects, pigment patterns are formed by melanin, ommochromes, and pteridines. Here, the effects of pteridine synthesis on melanin formation were studied using 4th instar larvae of a wild-type silkworm strain(dazao) with normal color and markings. Results from injected larvae and in vitro integument culture indicated that decreased activity of guanosine triphosphate cyclohydrolase Ⅰ(GTP-CH Ⅰ, a rate-limiting enzyme for pteridine synthesis), lowers BH4(6R-L-erythro-5,6,7,8-tetrahydrobiopterin, a production correlated with GTP-CH Ⅰ activity) levels and eliminates markings and coloration. The conversion of phenylalanine and tyrosine to melanin was prevented when GTP-CH Ⅰ was inhibited. When BH4 was added, phenylalanine was converted to tyrosine, and the tyrosine concentration increased. Tyrosine was then converted to melanin to create normal markings and coloration. Decreasing GTP-CH Ⅰ activity did not affect DOPA. GTP-CH Ⅰ affected melanin synthesis by generating the BH4 used in 2 key reaction steps:(1) conversion of phenylalanine to tyrosine by PAH(phenylalanine hydroxylase) and(2) conversion of tyrosine to DOPA by TH(tyrosine hydroxylase). Expression profiles of BmGTPCH Ia, BmGTPCH Ib, BmTH, and BmPAH in the integument were consistent with the current findings.
In insects, pigment patterns are formed by melanin, ommochromes, and pteridines. Here, the effects of pteridine synthesis on melanin formation were studied using 4th instar larvae of a wild-type silkworm strain(dazao) with normal color and markings. Results from injected larvae and in vitro integument culture indicated that decreased activity of guanosine triphosphate cyclohydrolase Ⅰ(GTP-CH Ⅰ, a rate-limiting enzyme for pteridine synthesis), lowers BH4(6R-L-erythro-5,6,7,8-tetrahydrobiopterin, a production correlated with GTP-CH Ⅰ activity) levels and eliminates markings and coloration. The conversion of phenylalanine and tyrosine to melanin was prevented when GTP-CH Ⅰ was inhibited. When BH4 was added, phenylalanine was converted to tyrosine, and the tyrosine concentration increased. Tyrosine was then converted to melanin to create normal markings and coloration. Decreasing GTP-CH Ⅰ activity did not affect DOPA. GTP-CH Ⅰ affected melanin synthesis by generating the BH4 used in 2 key reaction steps:(1) conversion of phenylalanine to tyrosine by PAH(phenylalanine hydroxylase) and(2) conversion of tyrosine to DOPA by TH(tyrosine hydroxylase). Expression profiles of BmGTPCH Ia, BmGTPCH Ib, BmTH, and BmPAH in the integument were consistent with the current findings.
In insects, pigment patterns are formed by melanin, ommochromes, and pteridines. Here, the effects of pteridine synthesis on melanin formation were studied using 4th instar larvae of a wild-type silkworm strain(dazao) with normal color and markings. Results from injected larvae and in vitro integument culture indicated that decreased activity of guanosine triphosphate cyclohydrolase Ⅰ(GTP-CH Ⅰ, a rate-limiting enzyme for pteridine synthesis), lowers BH4(6R-L-erythro-5,6,7,8-tetrahydrobiopterin, a production correlated with GTP-CH Ⅰ activity) levels and eliminates markings and coloration. The conversion of phenylalanine and tyrosine to melanin was prevented when GTP-CH Ⅰ was inhibited. When BH4 was added, phenylalanine was converted to tyrosine, and the tyrosine concentration increased. Tyrosine was then converted to melanin to create normal markings and coloration. Decreasing GTP-CH Ⅰ activity did not affect DOPA. GTP-CH Ⅰ affected melanin synthesis by generating the BH4 used in 2 key reaction steps:(1) conversion of phenylalanine to tyrosine by PAH(phenylalanine hydroxylase) and(2) conversion of tyrosine to DOPA by TH(tyrosine hydroxylase). Expression profiles of BmGTPCH Ia, BmGTPCH Ib, BmTH, and BmPAH in the integument were consistent with the current findings.
引文