Effects of Supplemental Boron on intestinal proliferation and apoptosis in African Ostrich Chicks
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- 英文论文题名：Effects of Supplemental Boron on intestinal proliferation and apoptosis in African Ostrich Chicks
- 论文作者：Pengpeng Sun ; Haseeb Khaliq ; Abdur Rahman Ansari ; Jing Wang ; Keli Yang ; Ke Xiao ; Kemei Peng
- 英文论文作者：Pengpeng Sun ; Haseeb Khaliq ; Abdur Rahman Ansari ; Jing Wang ; Keli Yang ; Ke Xiao ; Kemei Peng ; College of Veterinary Medicine ; Huazhong Agricultural University
- 年：2016
- 作者机构：College of Veterinary Medicine,Huazhong Agricultural University;
- 英文论文关键词：Apoptosis ; Boron ; Ostrich chicks ; Intestine ; PCNA
- 会议召开时间：2016-08-20
- 会议录名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会论文集
- 语种：英文
- 分类号：S839
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会
- 会议地点：中国河南郑州
- 主办单位：中国畜牧兽医学会动物解剖及组织胚胎学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：54k
- 原文格式：O
- 会议级别：全国
摘要
Boron is an essential trace element which plays an important role in process of metabolism of many biological compounds and the function of the tissues.The aim of this study was to investigate the effects of boron on the intestinal cell proliferation and apoptosis in African Ostrich chicks.A total of 36,ten day-old ostrich chicks were divided into six groups and fed on the same basal diet supplemented with 40,80,160,320 and 640 mg/L boric acid in drinking water for 80 days.Proliferating cell nuclear antigen(PCNA) was used to test the proliferation index of intestine in different group by immunohistochemical staining(IHC).Apoptotic cells of intestine were detected by means of TUNEL and evaluated by mean optical density.Results showed that proliferation of intestinal cells significantly increased in high dose of boron groups(160,320 and640 mg/L).TUNEL reaction showed that apoptosis significantly decreased in 40 and 80 mg/L groups,while significantly increased in high dose of boron groups(320 and 640 mg/L),especially in epithelium.In conclusion,low dose of boron-supplemented water could promote growth of intestinal villus by depressing apoptosis,while high dose of boron could cause intestinal apoptosis and thus we found increased proliferation of intestine cell as a compensatory adaption.These findings may support optimal dosage of boron that could protect the development of Ostrich intestine,while high dosage of boron could suppress it,or even has toxic effects on it.
Boron is an essential trace element which plays an important role in process of metabolism of many biological compounds and the function of the tissues.The aim of this study was to investigate the effects of boron on the intestinal cell proliferation and apoptosis in African Ostrich chicks.A total of 36,ten day-old ostrich chicks were divided into six groups and fed on the same basal diet supplemented with 40,80,160,320 and 640 mg/L boric acid in drinking water for 80 days.Proliferating cell nuclear antigen(PCNA) was used to test the proliferation index of intestine in different group by immunohistochemical staining(IHC).Apoptotic cells of intestine were detected by means of TUNEL and evaluated by mean optical density.Results showed that proliferation of intestinal cells significantly increased in high dose of boron groups(160,320 and640 mg/L).TUNEL reaction showed that apoptosis significantly decreased in 40 and 80 mg/L groups,while significantly increased in high dose of boron groups(320 and 640 mg/L),especially in epithelium.In conclusion,low dose of boron-supplemented water could promote growth of intestinal villus by depressing apoptosis,while high dose of boron could cause intestinal apoptosis and thus we found increased proliferation of intestine cell as a compensatory adaption.These findings may support optimal dosage of boron that could protect the development of Ostrich intestine,while high dosage of boron could suppress it,or even has toxic effects on it.
Boron is an essential trace element which plays an important role in process of metabolism of many biological compounds and the function of the tissues.The aim of this study was to investigate the effects of boron on the intestinal cell proliferation and apoptosis in African Ostrich chicks.A total of 36,ten day-old ostrich chicks were divided into six groups and fed on the same basal diet supplemented with 40,80,160,320 and 640 mg/L boric acid in drinking water for 80 days.Proliferating cell nuclear antigen(PCNA) was used to test the proliferation index of intestine in different group by immunohistochemical staining(IHC).Apoptotic cells of intestine were detected by means of TUNEL and evaluated by mean optical density.Results showed that proliferation of intestinal cells significantly increased in high dose of boron groups(160,320 and640 mg/L).TUNEL reaction showed that apoptosis significantly decreased in 40 and 80 mg/L groups,while significantly increased in high dose of boron groups(320 and 640 mg/L),especially in epithelium.In conclusion,low dose of boron-supplemented water could promote growth of intestinal villus by depressing apoptosis,while high dose of boron could cause intestinal apoptosis and thus we found increased proliferation of intestine cell as a compensatory adaption.These findings may support optimal dosage of boron that could protect the development of Ostrich intestine,while high dosage of boron could suppress it,or even has toxic effects on it.
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