BZP的LC-MS/MS定性定量分析方法
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- 英文论文题名：Qualitative and quantitative analysis of BZP By LC-MS/MS method
- 论文作者：常颖 ; 高利生
- 英文论文作者：Chang Ying ; Gao Li-sheng ; Beijing Engineering Research Center of Crime Scene Evidence Examination ; Institute of Forensic Science ; Ministry of Public Security P.R.C
- 年：2016
- 作者机构：公安部物证鉴定中心;北京市现场物证检验工程技术中心;
- 论文关键词：LC-MS/MS ; BZP ; 定性 ; 定量
- 英文论文关键词：LC-MS/MS ; BZP ; qualitative ; quantitative
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十四分会：公共安全化学
- 语种：中文
- 分类号：O657.63;D918.93
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十四分会 ; 公共安全化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：186k
- 原文格式：D
- 会议级别：全国
摘要
BZP属哌嗪类新精神活性物质,是一种合成的新型毒品,具有与MDMA相似的兴奋和致幻作用,已成为嗜毒者的替代迷幻药。2000年欧洲各国开始出现滥用,随后欧盟14个成员国都出现了BZP的滥用报道。目前常与其他毒品(如氯胺酮、甲基苯丙胺、摇头丸等)混合,增强致幻和兴奋作用,以片剂、粉末或液体的形式在娱乐场所交易。由于BZP毒副作用大,滥用日趋严重,引发的刑事犯罪等社会问题日益增多。正是出于实际办案的需要,我们建立了BZP的LC-MS/MS定性定量分析方法。该方法与已报道的方法相比具有灵敏度高,分析时间短,流动相消耗少,线性范围良好等优点。本方法采用Agilent 6460三重串联四极杆液质联用仪(LC/QQQ),Waters ACQUITY UPLC?BEH Phenyl色谱柱(150mm×2.1mm,1.7μm),流动相为0.1%甲酸和乙腈,梯度洗脱,流速为0.3mL/min。质谱应用ESI源、正离子模式、多反应监测(MRM)方式。本方法在0.1-10000 ng/mL浓度范围内线性关系良好R~2=0.999 8,日内与日间保留时间和峰面积的标准偏差(RSD)均<5.00%,检出限为0.06 ng/mL。该方法适用于BZP的定性定量分析。
To establish a qualitative and quantitative method for detecting BZP with LC-MS/MS. Using Agilent LC/QQQ,and Waters ACQUITY UPLC? BEH Phenyl column(150mm×2.1mm,1.7μm),mobile phase is 0.1% FA and Acetonitrile,Gradient elution,the flow rate is 0.3mL/min. Mass spectrometry using ESI source,positive ion mode,multiple reaction monitoring(MRM) mode detection concentration of BZP. This method has good linearity with the real value at the range from 0.1 to 10000ng/mL,R~2=0.999 8. Both intra-day and inter-day precisions expressed by relative standard deviations of retention time and peak area were less than 5.01%. The detectable limit was 0.04 ng/mL. This method was suitable for qualitative and quantitative analysis.
To establish a qualitative and quantitative method for detecting BZP with LC-MS/MS. Using Agilent LC/QQQ,and Waters ACQUITY UPLC? BEH Phenyl column(150mm×2.1mm,1.7μm),mobile phase is 0.1% FA and Acetonitrile,Gradient elution,the flow rate is 0.3mL/min. Mass spectrometry using ESI source,positive ion mode,multiple reaction monitoring(MRM) mode detection concentration of BZP. This method has good linearity with the real value at the range from 0.1 to 10000ng/mL,R~2=0.999 8. Both intra-day and inter-day precisions expressed by relative standard deviations of retention time and peak area were less than 5.01%. The detectable limit was 0.04 ng/mL. This method was suitable for qualitative and quantitative analysis.
引文
[1].New drug under formal scrutiny:Council asks EMCDDA to assess risks of BZP(PDF).European Monitoring Certre for Drugs and Drug Addiction.23 March 2007.Retrieved 2007-04-14.
[2].Summary of response:Consultation on proposed control under the Misuse of Drugs Act 1971 of1-Benzylpiperazine and a group of substituted piperazines.Homeoffice.gov.uk.30 October 2009.Retrieved2010-01-01.
[3].Misuse of Drugs(Classification of BZP)Amendment Act 2008,Wellington,New Zealand.
[2].Summary of response:Consultation on proposed control under the Misuse of Drugs Act 1971 of1-Benzylpiperazine and a group of substituted piperazines.Homeoffice.gov.uk.30 October 2009.Retrieved2010-01-01.
[3].Misuse of Drugs(Classification of BZP)Amendment Act 2008,Wellington,New Zealand.