Eicosanoids mediate sHSP 20.8 gene response to biotic stress in larvae of the Chinese oak silkworm Antheraea pernyi
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- 英文论文题名：Eicosanoids mediate sHSP 20.8 gene response to biotic stress in larvae of the Chinese oak silkworm Antheraea pernyi
- 论文作者：Zhang Cong-Fen ; Dai Li-Shang ; Wang Lei ; Qian Cen ; Wei Guo-Qing ; Li Jun ; Zhu Bao-Jian ; Liu Chao-Liang
- 英文论文作者：Zhang Cong-Fen ; Dai Li-Shang ; Wang Lei ; Qian Cen ; Wei Guo-Qing ; Li Jun ; Zhu Bao-Jian ; Liu Chao-Liang ; College of Life Science ; Anhui Agricultural University
- 年：2016
- 作者机构：College of Life Science, Anhui Agricultural University;
- 英文论文关键词：Antheraea pernyi ; Small heat shock protein 20.8 ; Eicosanoids
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S885.1
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：497k
- 原文格式：D
- 会议级别：全国
摘要
Small heat shock proteins(s HSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi(A. pernyi; Lepidoptera: Saturniidae), cDNA encoding HSP20.8 in A. pernyi, termed Ap-sHSP20.8, was identified as a 564 bp ORF. The translated amino acid sequence encoded 187 residues with a calculated molecular mass of 20.8 kDa and an isoelectronic point(p I) of 5.98; the sequence showed homology to s HSP chaperone proteins from other insects. Ap-s HSP20.8 mRNA transcript expression was abundant in the midgut and fat body and found to be both constitutive and inducible by infectious stimuli. Therefore, Ap-s HSP20.8 may play important roles in A. pernyi immune responses under biotic stress. Furthermore, we found that eicosanoids could mediate the induction of Ap-sHSP20.8 in the fat body andmidgut. Our findings show that s HSPs may be promising molecules to target in order to cripple immunity in insect pests.
Small heat shock proteins(s HSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi(A. pernyi; Lepidoptera: Saturniidae), cDNA encoding HSP20.8 in A. pernyi, termed Ap-sHSP20.8, was identified as a 564 bp ORF. The translated amino acid sequence encoded 187 residues with a calculated molecular mass of 20.8 kDa and an isoelectronic point(p I) of 5.98; the sequence showed homology to s HSP chaperone proteins from other insects. Ap-s HSP20.8 mRNA transcript expression was abundant in the midgut and fat body and found to be both constitutive and inducible by infectious stimuli. Therefore, Ap-s HSP20.8 may play important roles in A. pernyi immune responses under biotic stress. Furthermore, we found that eicosanoids could mediate the induction of Ap-sHSP20.8 in the fat body andmidgut. Our findings show that s HSPs may be promising molecules to target in order to cripple immunity in insect pests.
Small heat shock proteins(s HSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi(A. pernyi; Lepidoptera: Saturniidae), cDNA encoding HSP20.8 in A. pernyi, termed Ap-sHSP20.8, was identified as a 564 bp ORF. The translated amino acid sequence encoded 187 residues with a calculated molecular mass of 20.8 kDa and an isoelectronic point(p I) of 5.98; the sequence showed homology to s HSP chaperone proteins from other insects. Ap-s HSP20.8 mRNA transcript expression was abundant in the midgut and fat body and found to be both constitutive and inducible by infectious stimuli. Therefore, Ap-s HSP20.8 may play important roles in A. pernyi immune responses under biotic stress. Furthermore, we found that eicosanoids could mediate the induction of Ap-sHSP20.8 in the fat body andmidgut. Our findings show that s HSPs may be promising molecules to target in order to cripple immunity in insect pests.
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