Haemonchus contortus excretory and secretory proteins(HcESPs) suppress functions of goat PBMCs in vitro
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- 英文论文题名：Haemonchus contortus excretory and secretory proteins(HcESPs) suppress functions of goat PBMCs in vitro
- 论文作者：Javaid Ali Gadahi ; Yongqian Bu ; Muhammad Ehsan ; Zhenchao Zhang ; Shuai Wang ; Ruofeng Yan ; Xiaokai Song ; Lixin Xu ; Xiangrui Li
- 英文论文作者：Javaid Ali Gadahi ; Yongqian Bu ; Muhammad Ehsan ; Zhenchao Zhang ; Shuai Wang ; Ruofeng Yan ; Xiaokai Song ; Lixin Xu ; Xiangrui Li ; College of Veterinary Medicine ; Nanjing Agricultural University
- 年：2016
- 作者机构：College of Veterinary Medicine, Nanjing Agricultural University;
- 英文论文关键词：Haemonchus contortus ; ESP ; goat ; PBMC ; immunomodulation
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：S858.27
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：153k
- 原文格式：D
- 会议级别：全国
摘要
Excretory and secretory products(ESPs) of nematode contain various proteins which are capable of inducing the instigation or depression of the host immune response and are involved in the pathogenesis of the worms. In the present study, Haemonchus contortus excretory and secretory products(Hc ESPs) were collected from the adult worms. Binding of Hc ESPs to goat peripheral blood mononuclear cells(PBMCs) was confirmed by immune-fluorescence assay. Effects of the Hc ESPs on cytokine production, cell proliferation, cell migration and nitric oxide(NO) production of PBMCs were checked by co-incubation of Hc ESPs with goat PBMCs. The results indicated that the production of IL-4 and IFN-γ were significantly decreased by Hc ESPs in dose dependent manner. On the contrary, the production of IL-10 and IL-17 were increased. Cell migration was significantly enhanced by Hc ESPs, whereas, Hc ESPs treatment significantly suppressed the cell proliferation and NO production. These results indicated that the Hc ESPs played important suppressive regulatory roles on PBMCs and provided highlights to the understanding of the host-parasite interactions.
Excretory and secretory products(ESPs) of nematode contain various proteins which are capable of inducing the instigation or depression of the host immune response and are involved in the pathogenesis of the worms. In the present study, Haemonchus contortus excretory and secretory products(Hc ESPs) were collected from the adult worms. Binding of Hc ESPs to goat peripheral blood mononuclear cells(PBMCs) was confirmed by immune-fluorescence assay. Effects of the Hc ESPs on cytokine production, cell proliferation, cell migration and nitric oxide(NO) production of PBMCs were checked by co-incubation of Hc ESPs with goat PBMCs. The results indicated that the production of IL-4 and IFN-γ were significantly decreased by Hc ESPs in dose dependent manner. On the contrary, the production of IL-10 and IL-17 were increased. Cell migration was significantly enhanced by Hc ESPs, whereas, Hc ESPs treatment significantly suppressed the cell proliferation and NO production. These results indicated that the Hc ESPs played important suppressive regulatory roles on PBMCs and provided highlights to the understanding of the host-parasite interactions.
Excretory and secretory products(ESPs) of nematode contain various proteins which are capable of inducing the instigation or depression of the host immune response and are involved in the pathogenesis of the worms. In the present study, Haemonchus contortus excretory and secretory products(Hc ESPs) were collected from the adult worms. Binding of Hc ESPs to goat peripheral blood mononuclear cells(PBMCs) was confirmed by immune-fluorescence assay. Effects of the Hc ESPs on cytokine production, cell proliferation, cell migration and nitric oxide(NO) production of PBMCs were checked by co-incubation of Hc ESPs with goat PBMCs. The results indicated that the production of IL-4 and IFN-γ were significantly decreased by Hc ESPs in dose dependent manner. On the contrary, the production of IL-10 and IL-17 were increased. Cell migration was significantly enhanced by Hc ESPs, whereas, Hc ESPs treatment significantly suppressed the cell proliferation and NO production. These results indicated that the Hc ESPs played important suppressive regulatory roles on PBMCs and provided highlights to the understanding of the host-parasite interactions.
引文