Fine regulation of ABA signaling and drought resistance by two b ZIP transcription factors in rice
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- 英文论文题名：Fine regulation of ABA signaling and drought resistance by two b ZIP transcription factors in rice
- 论文作者：Ning Tang ; Wei Zong ; Siqi Ma ; Lizhong Xiong
- 英文论文作者：Ning Tang ; Wei Zong ; Siqi Ma ; Lizhong Xiong ; National Key Laboratory of Crop Genetic Improvement ; Huazhong Agricultual University
- 年：2016
- 作者机构：National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultual University;
- 英文论文关键词：abscisic acid ; drought ; Oryza sativa ; transcriptional regulation
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：841k
- 原文格式：D
- 会议级别：全国
摘要
Plants have evolved complicated mechanisms to survive adverse environmental conditions. Previously,we reported that transcription factors Osb ZIP23 and Osb ZIP46 regulates abscisic acid(ABA) signalingmediated drought tolerance in rice. Here we present that Osb ZIP23 directly regulates a large number of reported genes that function in stress response,hormone signaling,and developmental processes. Among these targets,we found that Osb ZIP23 could positively regulate Os PP2C49,and overexpression of Os PP2C49 in rice resulted in significantly decreased sensitivity of the ABA response and rapid dehydration. Moreover,Os NCED4(9-cis-epoxycarotenoid dioxygenase 4),a key gene in ABA biosynthesis,was also positively regulated by Osb ZIP23. These results suggest that Osb ZIP23 acts as a central regulator in ABA signaling and biosynthesis,and drought resistance in rice. Meanwhile,we found that the activation of Osb ZIP46 is strikingly repressed by an intrinsic domain D. An Osb ZIP46-in teracting protein MODD was found to be a mediator of Osb ZIP46 deactivation and degradation. MODD negatively regulates ABA signaling and drought stress tolerance,and inhibits the expression of the Osb ZIP46 target genes. MODD represses the activity of Osb ZIP46 via interaction with the Os TPR3-HDA702 co-repressor complex and down-regulation of the histone acetylation level at the target genes of Osb ZIP46. MODD promotes Osb ZIP46 degradation via interaction with an ubiquitination E3 ligase Os PUB70. Interestingly,the D domain is required for both the deactivation and degradation of Osb ZIP46 via interaction with MODD. These findings exemplify that plants employ elaborate transcriptional regulatory mechanisms to fine-tune the drought responses.
Plants have evolved complicated mechanisms to survive adverse environmental conditions. Previously,we reported that transcription factors Osb ZIP23 and Osb ZIP46 regulates abscisic acid(ABA) signalingmediated drought tolerance in rice. Here we present that Osb ZIP23 directly regulates a large number of reported genes that function in stress response,hormone signaling,and developmental processes. Among these targets,we found that Osb ZIP23 could positively regulate Os PP2C49,and overexpression of Os PP2C49 in rice resulted in significantly decreased sensitivity of the ABA response and rapid dehydration. Moreover,Os NCED4(9-cis-epoxycarotenoid dioxygenase 4),a key gene in ABA biosynthesis,was also positively regulated by Osb ZIP23. These results suggest that Osb ZIP23 acts as a central regulator in ABA signaling and biosynthesis,and drought resistance in rice. Meanwhile,we found that the activation of Osb ZIP46 is strikingly repressed by an intrinsic domain D. An Osb ZIP46-in teracting protein MODD was found to be a mediator of Osb ZIP46 deactivation and degradation. MODD negatively regulates ABA signaling and drought stress tolerance,and inhibits the expression of the Osb ZIP46 target genes. MODD represses the activity of Osb ZIP46 via interaction with the Os TPR3-HDA702 co-repressor complex and down-regulation of the histone acetylation level at the target genes of Osb ZIP46. MODD promotes Osb ZIP46 degradation via interaction with an ubiquitination E3 ligase Os PUB70. Interestingly,the D domain is required for both the deactivation and degradation of Osb ZIP46 via interaction with MODD. These findings exemplify that plants employ elaborate transcriptional regulatory mechanisms to fine-tune the drought responses.
Plants have evolved complicated mechanisms to survive adverse environmental conditions. Previously,we reported that transcription factors Osb ZIP23 and Osb ZIP46 regulates abscisic acid(ABA) signalingmediated drought tolerance in rice. Here we present that Osb ZIP23 directly regulates a large number of reported genes that function in stress response,hormone signaling,and developmental processes. Among these targets,we found that Osb ZIP23 could positively regulate Os PP2C49,and overexpression of Os PP2C49 in rice resulted in significantly decreased sensitivity of the ABA response and rapid dehydration. Moreover,Os NCED4(9-cis-epoxycarotenoid dioxygenase 4),a key gene in ABA biosynthesis,was also positively regulated by Osb ZIP23. These results suggest that Osb ZIP23 acts as a central regulator in ABA signaling and biosynthesis,and drought resistance in rice. Meanwhile,we found that the activation of Osb ZIP46 is strikingly repressed by an intrinsic domain D. An Osb ZIP46-in teracting protein MODD was found to be a mediator of Osb ZIP46 deactivation and degradation. MODD negatively regulates ABA signaling and drought stress tolerance,and inhibits the expression of the Osb ZIP46 target genes. MODD represses the activity of Osb ZIP46 via interaction with the Os TPR3-HDA702 co-repressor complex and down-regulation of the histone acetylation level at the target genes of Osb ZIP46. MODD promotes Osb ZIP46 degradation via interaction with an ubiquitination E3 ligase Os PUB70. Interestingly,the D domain is required for both the deactivation and degradation of Osb ZIP46 via interaction with MODD. These findings exemplify that plants employ elaborate transcriptional regulatory mechanisms to fine-tune the drought responses.
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