HAF1 mediates circadian accumulation of Os ELF3 in determining the heading date of rice under long-day conditions
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- 英文论文题名：HAF1 mediates circadian accumulation of Os ELF3 in determining the heading date of rice under long-day conditions
- 论文作者：Chunmei Zhu ; Qiang Peng ; Debao Fu ; Dongxia Zhuang ; Changyin Wu
- 英文论文作者：Chunmei Zhu ; Qiang Peng ; Debao Fu ; Dongxia Zhuang ; Changyin Wu ; National Key Laboratory of Crop Genetic Improvement ; Huazhong Agricultural University
- 年：2016
- 作者机构：National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University;
- 英文论文关键词：circadian clock ; photoperiodic flowering ; ubiquitination ; rice
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S511
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：841k
- 原文格式：D
- 会议级别：全国
摘要
Plant circadian clock has been provided to be involved in multiple physiology and metabolism process by monitoring external environment changes,including photoperiodic flowering regulation. Our previous study showed that Os ELF3 acts as a floral activator in the long-day photoperiodic pathway via its crosstalk with the circadian clock in rice. However,post-transcription regulation of Os ELF3 is still unclear. In this study,we showed that HAF1,an E3 ubiquitin ligase,physically interact with Os ELF3 in vivo and in vitro. C-terminal domain of Os ELF3 was identified as the functional motif for mediating interaction with HAF1,as well as formation of homodimer by itself. Further protein degradation assay indicated that Os ELF3 is the direct substrate of HAF1 for ubiquitination in rice. Genetically,the oself3 haf1 double mutant headed as late as oself3 under long-day conditions. Previous investigation revealed that Os ELF3 plays a crucial role in photoperiodic response to determine rice regional adaptability. We proposed that HAF1 plays a pivotal role in heading date modulation by ubiquitination of Os ELF3 under long-day conditions.
Plant circadian clock has been provided to be involved in multiple physiology and metabolism process by monitoring external environment changes,including photoperiodic flowering regulation. Our previous study showed that Os ELF3 acts as a floral activator in the long-day photoperiodic pathway via its crosstalk with the circadian clock in rice. However,post-transcription regulation of Os ELF3 is still unclear. In this study,we showed that HAF1,an E3 ubiquitin ligase,physically interact with Os ELF3 in vivo and in vitro. C-terminal domain of Os ELF3 was identified as the functional motif for mediating interaction with HAF1,as well as formation of homodimer by itself. Further protein degradation assay indicated that Os ELF3 is the direct substrate of HAF1 for ubiquitination in rice. Genetically,the oself3 haf1 double mutant headed as late as oself3 under long-day conditions. Previous investigation revealed that Os ELF3 plays a crucial role in photoperiodic response to determine rice regional adaptability. We proposed that HAF1 plays a pivotal role in heading date modulation by ubiquitination of Os ELF3 under long-day conditions.
Plant circadian clock has been provided to be involved in multiple physiology and metabolism process by monitoring external environment changes,including photoperiodic flowering regulation. Our previous study showed that Os ELF3 acts as a floral activator in the long-day photoperiodic pathway via its crosstalk with the circadian clock in rice. However,post-transcription regulation of Os ELF3 is still unclear. In this study,we showed that HAF1,an E3 ubiquitin ligase,physically interact with Os ELF3 in vivo and in vitro. C-terminal domain of Os ELF3 was identified as the functional motif for mediating interaction with HAF1,as well as formation of homodimer by itself. Further protein degradation assay indicated that Os ELF3 is the direct substrate of HAF1 for ubiquitination in rice. Genetically,the oself3 haf1 double mutant headed as late as oself3 under long-day conditions. Previous investigation revealed that Os ELF3 plays a crucial role in photoperiodic response to determine rice regional adaptability. We proposed that HAF1 plays a pivotal role in heading date modulation by ubiquitination of Os ELF3 under long-day conditions.
引文