Micro RNA-7 deficiency ameliorates the pathologies of Acute Lung Injury through Elevating KLF4
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- 英文论文题名：Micro RNA-7 deficiency ameliorates the pathologies of Acute Lung Injury through Elevating KLF4
- 论文作者：Zhao Juanjuan ; Chen Chao ; Guo Mengmeng ; Xu Hualin ; Xu Lin
- 英文论文作者：Zhao Juanjuan ; Chen Chao ; Guo Mengmeng ; Xu Hualin ; Xu Lin ; Department of immunology ; Zunyi Medical College
- 年：2016
- 作者机构：Department of immunology,Zunyi Medical College;
- 英文论文关键词：miR-7KD ; acute lung injury ; immune cells ; cytokines ; KLF4
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会壁报交流集
- 英文会议录名称：Proceedings of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R563
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1348k
- 原文格式：D
- 会议级别：全国
摘要
Recent evidence showed that micro RNA-7(mi R-7) played an important role in the pathologies of lungrelated diseases.However,the potential role of mi R-7 in acute lung injury(ALI) still remains poorly understood.Here we assessed the effect of mi R-7 deficiency on the pathology of ALI.We firstly found that the expression of mi R-7 was upregulated in lung tissue in murine LPS-induced ALI model.Notably,we generated mi R-7 knock down(KD) mice byusing mi RNA-Sponge technique and found that mi R-7 deficiency could ameliorate the pathologies of lung as evidenced by accelerated body weight recovery,reduced level of bronchoalveolar lavage(BAL) proinflammatory cytokines and decreased number of BAL cells in ALI mice.Moreover,the proportion and number of various immune cells in BAL,including innate immune cell F4/80+macrophages,γδT cells,NK1.1+T cells and CD11c+DCs,as well as adaptive immune cell CD4+T cells and CD8+T cells,also significantly changed respectively.Mechanistic evidence showed that KLF4,a target molecule of mi R-7,was upregulated in lung tissues in ALI model,accompanied by altered transduction of NF-k B,AKT and ERK pathway.These data provided a previously unknown role of mi R-7 in pathology of ALI,which could ultimately aid the understanding of development of ALI and the development of new therapeutic strategies against clinical inflammatory lung diseases.
Recent evidence showed that micro RNA-7(mi R-7) played an important role in the pathologies of lungrelated diseases.However,the potential role of mi R-7 in acute lung injury(ALI) still remains poorly understood.Here we assessed the effect of mi R-7 deficiency on the pathology of ALI.We firstly found that the expression of mi R-7 was upregulated in lung tissue in murine LPS-induced ALI model.Notably,we generated mi R-7 knock down(KD) mice byusing mi RNA-Sponge technique and found that mi R-7 deficiency could ameliorate the pathologies of lung as evidenced by accelerated body weight recovery,reduced level of bronchoalveolar lavage(BAL) proinflammatory cytokines and decreased number of BAL cells in ALI mice.Moreover,the proportion and number of various immune cells in BAL,including innate immune cell F4/80+macrophages,γδT cells,NK1.1+T cells and CD11c+DCs,as well as adaptive immune cell CD4+T cells and CD8+T cells,also significantly changed respectively.Mechanistic evidence showed that KLF4,a target molecule of mi R-7,was upregulated in lung tissues in ALI model,accompanied by altered transduction of NF-k B,AKT and ERK pathway.These data provided a previously unknown role of mi R-7 in pathology of ALI,which could ultimately aid the understanding of development of ALI and the development of new therapeutic strategies against clinical inflammatory lung diseases.
Recent evidence showed that micro RNA-7(mi R-7) played an important role in the pathologies of lungrelated diseases.However,the potential role of mi R-7 in acute lung injury(ALI) still remains poorly understood.Here we assessed the effect of mi R-7 deficiency on the pathology of ALI.We firstly found that the expression of mi R-7 was upregulated in lung tissue in murine LPS-induced ALI model.Notably,we generated mi R-7 knock down(KD) mice byusing mi RNA-Sponge technique and found that mi R-7 deficiency could ameliorate the pathologies of lung as evidenced by accelerated body weight recovery,reduced level of bronchoalveolar lavage(BAL) proinflammatory cytokines and decreased number of BAL cells in ALI mice.Moreover,the proportion and number of various immune cells in BAL,including innate immune cell F4/80+macrophages,γδT cells,NK1.1+T cells and CD11c+DCs,as well as adaptive immune cell CD4+T cells and CD8+T cells,also significantly changed respectively.Mechanistic evidence showed that KLF4,a target molecule of mi R-7,was upregulated in lung tissues in ALI model,accompanied by altered transduction of NF-k B,AKT and ERK pathway.These data provided a previously unknown role of mi R-7 in pathology of ALI,which could ultimately aid the understanding of development of ALI and the development of new therapeutic strategies against clinical inflammatory lung diseases.
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