铜绿假单胞菌YY24对凡纳滨对虾养殖水体水质的影响
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摘要
本试验以初始平均体重为(5.75±0.5)g的凡纳滨对虾为研究对象,探讨了水体中不同浓度投放铜绿假单胞菌(Pseudomonas aeruginosa)(YY24)对凡纳滨对虾的养殖水体水质的影响。添加不同比例浓度的YY24和商品菌研究凡纳滨对虾水质指标的影响。试验设10个处理,每个处理3个重复,每个重复30尾,试验周期是10 d,投放频率均为2 d,并保持其活性完好,有效养殖水体(50±3)L,控制温度(28±1)℃,盐度(20±1)‰,每日每次每箱投料量按总重量的1%,氧气充足。试验期间,每24 h测定水体基础指标;在试验第0、12、24、48、96、144、168、192、240 h测定水体理化指标。试验结果表明:与对照组相比,各试验组均能明显净化水质;试验组的pH呈上升趋势;各试验组NH4+-N、NO3--N、NO2--N浓度均在96 h时就达到最低值;添加浓度为106 cfu/mLYY24的投放方式的效果最好,使水体中无机氮维持在相对较低水平。
The initial average weight was(5.75±0.5) g of Litopenaeus vannamei as the research object, discusses the addition of Pseudomonas bacteria(Pseudomonas aeruginosa)(YY24) to the effect of water quality aquaculture pool in Litopenaeus vannamei of different delivery concentration. The effect of adding different concentration ratios of YY24 and Commercial bacteria on Litopenaeus vannamei quality index. Experiment with 10 treatments, 3 replicates per treatment, each repeated 30 tail, test period is 10 d, the delivery frequency is every 2 d, and keep its activity in good condition, the effective water is(50±3) L, temperature is(28±1)℃, salinity is(20±1)%, the daily feeding amount of each box according to the 1% of total weight,oxygen is enough. During the experiment, the basic indicators of water bodies were determined every 24 h;physical and chemical indicators of water body were determined in 0,12, 24, 48, 96, 144, 168, 192,240 h. The test results showed that: compared with the control group, each experimental groups can obviously purify water, the test group pH showed a rising trend, each experimental group NH4+-N, NO3--N and NO2--N reaching the lowest value ON 96 h, in summary, the experimental group of add a concentration of 106 cfu/mLYY24 was the best and maintained the inorganic nitrogen in water at a relatively low level.
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