Bombyx mori nucleopolyhedrovirus ORF79 is a per os infectivity factor associated with the PIF complex
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- 英文论文题名：Bombyx mori nucleopolyhedrovirus ORF79 is a per os infectivity factor associated with the PIF complex
- 论文作者：Zhan-Qi Dong ; Jun zhang ; Xue-Mei Chen ; Qian He ; Ming-Ya Cao ; La Wang ; Hai-Qing Li ; Wen-Fu Xiao ; Cai-Xia Pan ; Cheng Lu ; Min-Hui Pan
- 英文论文作者：Zhan-Qi Dong ; Jun zhang ; Xue-Mei Chen ; Qian He ; Ming-Ya Cao ; La Wang ; Hai-Qing Li ; Wen-Fu Xiao ; Cai-Xia Pan ; Cheng Lu ; Min-Hui Pan ; State Key Laboratory of Silkworm Genome Biology ; Southwest University ; Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry ; Southwest University
- 年：2016
- 作者机构：State Key Laboratory of Silkworm Genome Biology, Southwest University;Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University;
- 英文论文关键词：Bombyx mori ; BmNPV ; Bm79 ; Per os infection ; PIF complex
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S884.51
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：508k
- 原文格式：D
- 会议级别：全国
摘要
Bombyx mori nucleopolyhedrovirus(BmNPV) ORF79(Bm79) encodes an occlusion-derived virus(ODV)-specific envelope protein, which is a homologue of the per os infectivity factor 4(PIF4) of Autographa californica multiple nucleopolyhedrovirus(Ac MNPV). To investigate the role of ORF79 in the BmNPV life cycle, a Bm79 knockout virus(vBmBm79KO) was constructed through homologous recombination in Escherichia coli. Viral DNA replication, budded virus(BV) production and polyhedral formation were unaffected by the absence of BM79. However, results of the larval bioassay demonstrated that the Bm79 deletion resulted in a complete loss of per os infection. Immunofluorescence analysis showed that BM79 localized at the innernuclear membrane of infected cells through its N-terminal sorting motif(SM). Further bimolecular fluorescence protein complementation and co-immunoprecipitation assays demonstrated the interaction of BM79 with PIF1, PIF2, PIF3 and ODV-E66. Thus, BM79 plays an important role in per os infection and is associated with the viral PIF complex of Bm NPV.
Bombyx mori nucleopolyhedrovirus(BmNPV) ORF79(Bm79) encodes an occlusion-derived virus(ODV)-specific envelope protein, which is a homologue of the per os infectivity factor 4(PIF4) of Autographa californica multiple nucleopolyhedrovirus(Ac MNPV). To investigate the role of ORF79 in the BmNPV life cycle, a Bm79 knockout virus(vBmBm79KO) was constructed through homologous recombination in Escherichia coli. Viral DNA replication, budded virus(BV) production and polyhedral formation were unaffected by the absence of BM79. However, results of the larval bioassay demonstrated that the Bm79 deletion resulted in a complete loss of per os infection. Immunofluorescence analysis showed that BM79 localized at the innernuclear membrane of infected cells through its N-terminal sorting motif(SM). Further bimolecular fluorescence protein complementation and co-immunoprecipitation assays demonstrated the interaction of BM79 with PIF1, PIF2, PIF3 and ODV-E66. Thus, BM79 plays an important role in per os infection and is associated with the viral PIF complex of Bm NPV.
Bombyx mori nucleopolyhedrovirus(BmNPV) ORF79(Bm79) encodes an occlusion-derived virus(ODV)-specific envelope protein, which is a homologue of the per os infectivity factor 4(PIF4) of Autographa californica multiple nucleopolyhedrovirus(Ac MNPV). To investigate the role of ORF79 in the BmNPV life cycle, a Bm79 knockout virus(vBmBm79KO) was constructed through homologous recombination in Escherichia coli. Viral DNA replication, budded virus(BV) production and polyhedral formation were unaffected by the absence of BM79. However, results of the larval bioassay demonstrated that the Bm79 deletion resulted in a complete loss of per os infection. Immunofluorescence analysis showed that BM79 localized at the innernuclear membrane of infected cells through its N-terminal sorting motif(SM). Further bimolecular fluorescence protein complementation and co-immunoprecipitation assays demonstrated the interaction of BM79 with PIF1, PIF2, PIF3 and ODV-E66. Thus, BM79 plays an important role in per os infection and is associated with the viral PIF complex of Bm NPV.
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