Decreased microbial diversity and Lactobacillus group in the intestine of geriatric giant pandas(Ailuropoda melanoleuca)
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- 英文论文题名：Decreased microbial diversity and Lactobacillus group in the intestine of geriatric giant pandas(Ailuropoda melanoleuca)
- 论文作者：Zhirong Peng ; Dong Zeng ; Qiang Wang ; Lili Niu ; Xueqin Ni ; Fuqin Zou ; Mingyue Yang ; Hao Sun ; Yi Zhou ; Qian Liu ; Zhongqiong Yin ; Kangcheng Pan ; Bo Jing
- 英文论文作者：Zhirong Peng ; Dong Zeng ; Qiang Wang ; Lili Niu ; Xueqin Ni ; Fuqin Zou ; Mingyue Yang ; Hao Sun ; Yi Zhou ; Qian Liu ; Zhongqiong Yin ; Kangcheng Pan ; Bo Jing ; Animal Microecology Institute ; College of Veterinary Medicine ; Sichuan Agricultural University ; Key Laboratory of Animal Disease and Human Health of Sichuan Province ; Chengdu Wildlife Institute ; Chengdu Zoo
- 年：2016
- 作者机构：Animal Microecology Institute,College of Veterinary Medicine,Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province;Chengdu Wildlife Institute,Chengdu Zoo;
- 英文论文关键词：Captive giant panda ; Diet ; Intestine microflora ; PCR-DGGE ; Real-time PCR
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：英文
- 分类号：S865.3
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：72k
- 原文格式：O
- 会议级别：全国
摘要
It has been established beyond doubt that giant panda genome lacks lignin-degrading related enzyme,gastrointestinal microbes may play a vital role in digestion of highly fibrous bamboo diet.However,there is not much information available about the intestinal bacteria composition in captive giant pandas with different ages.In this study,we compared the intestinal bacterial community of 12 captive giant pandas from three different age groups(subadults,adults,and geriatrics) through PCR-denaturing gradient gel electrophoresis(DGGE) and real-time PCR analysis.Results indicated that microbial diversity in the intestine of adults was significantly higher than that of the geriatrics(p < 0.05),but not significant compared to the subadults(p > 0.05).The predominant bands in DGGE patterns shared by the twelve pandas were related to Firmicutes and Proteobacteria.Additionally,in comparison to healthy individuals,antibiotic-treated animals showed partial microbial dysbiosis.Real-time PCR analyses confirmed a significantly higher abundance of the Lactobacillus in the fecal microbiota of adults(p < 0.05),while other bacterial groups and species detected did not significantly differ among the three age groups(p > 0.05).This study revealed that captive giant pandas with different ages showed different intestinal bacteria composition.
It has been established beyond doubt that giant panda genome lacks lignin-degrading related enzyme,gastrointestinal microbes may play a vital role in digestion of highly fibrous bamboo diet.However,there is not much information available about the intestinal bacteria composition in captive giant pandas with different ages.In this study,we compared the intestinal bacterial community of 12 captive giant pandas from three different age groups(subadults,adults,and geriatrics) through PCR-denaturing gradient gel electrophoresis(DGGE) and real-time PCR analysis.Results indicated that microbial diversity in the intestine of adults was significantly higher than that of the geriatrics(p < 0.05),but not significant compared to the subadults(p > 0.05).The predominant bands in DGGE patterns shared by the twelve pandas were related to Firmicutes and Proteobacteria.Additionally,in comparison to healthy individuals,antibiotic-treated animals showed partial microbial dysbiosis.Real-time PCR analyses confirmed a significantly higher abundance of the Lactobacillus in the fecal microbiota of adults(p < 0.05),while other bacterial groups and species detected did not significantly differ among the three age groups(p > 0.05).This study revealed that captive giant pandas with different ages showed different intestinal bacteria composition.
It has been established beyond doubt that giant panda genome lacks lignin-degrading related enzyme,gastrointestinal microbes may play a vital role in digestion of highly fibrous bamboo diet.However,there is not much information available about the intestinal bacteria composition in captive giant pandas with different ages.In this study,we compared the intestinal bacterial community of 12 captive giant pandas from three different age groups(subadults,adults,and geriatrics) through PCR-denaturing gradient gel electrophoresis(DGGE) and real-time PCR analysis.Results indicated that microbial diversity in the intestine of adults was significantly higher than that of the geriatrics(p < 0.05),but not significant compared to the subadults(p > 0.05).The predominant bands in DGGE patterns shared by the twelve pandas were related to Firmicutes and Proteobacteria.Additionally,in comparison to healthy individuals,antibiotic-treated animals showed partial microbial dysbiosis.Real-time PCR analyses confirmed a significantly higher abundance of the Lactobacillus in the fecal microbiota of adults(p < 0.05),while other bacterial groups and species detected did not significantly differ among the three age groups(p > 0.05).This study revealed that captive giant pandas with different ages showed different intestinal bacteria composition.
引文
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Alonso S,Laca A,Rendueles M,Mayo B,Diaz M(2015)Cider Apple Native Microbiota Characterization by PCR-DGGE.JI Brewing 121(2):287-289.doi:10.1002/jib.220
Altenhoevel A,Norman K,Smoliner C,Peroz I(2012)The impact of self-perceived masticatory function on nutrition and gastrointestinal complaints in the elderly.J Nutr Health Aging 16(2):175-178.doi:10.1007/s 12603-011-0342-8
Bartosch S,Fite A,Macfarlane GT,Mcmurdo ME(2004)Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota.Appl Environ Microbiol 70(6):3575-3581.doi:10.1128/AEM.70.6.3575-3581.2004
Alonso S,Laca A,Rendueles M,Mayo B,Diaz M(2015)Cider Apple Native Microbiota Characterization by PCR-DGGE.JI Brewing 121(2):287-289.doi:10.1002/jib.220
Altenhoevel A,Norman K,Smoliner C,Peroz I(2012)The impact of self-perceived masticatory function on nutrition and gastrointestinal complaints in the elderly.J Nutr Health Aging 16(2):175-178.doi:10.1007/s 12603-011-0342-8
Bartosch S,Fite A,Macfarlane GT,Mcmurdo ME(2004)Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota.Appl Environ Microbiol 70(6):3575-3581.doi:10.1128/AEM.70.6.3575-3581.2004