分子印迹-流动注射分子荧光法测水中的双酚A
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- 英文论文题名：Molecularly imprined technique-flow injection-molecular fluorescence Spectrometry detect BPA in water
- 论文作者：袁堂蜜 ; 李文佐 ; 刘振波
- 英文论文作者：Tangmi Yuan ; Wenzuo Li ; Zhenbo Liu ; College of Chemistry and Chemical Engineering ; Yantai University
- 年：2016
- 作者机构：烟台大学化学化工学院;
- 论文关键词：分子印迹聚合物 ; 分子荧光 ; 双酚A
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十七分会：光化学
- 语种：中文
- 分类号：O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十七分会 ; 光化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：256k
- 原文格式：D
- 会议级别：全国
摘要
双酚A(BPA)是一种常见的环境激素,会干扰生物体正常的内分泌,从而危害人类身体健康。目前BPA测定方法众多,但往往选择性差、样品前处理复杂,耗时长。分子印迹技术能够选择性的识别模板分子,具备可识别性和可预定性等优点,被广泛应用于固相萃取技术、手性药物拆分、环境样品分析、色谱分离等领域。本文采用沉淀聚合法,以双酚A为模板分子,β-环糊精为功能单体,二甲基亚砜为溶剂,六亚甲基二异氰酸酯为交联剂,偶氮二异丁腈为引发剂,合成了BPA的分子印迹聚合物(BPA-MIPs)。实验制得的BPA-MIPs能很好的识别模版分子双酚A,平衡吸附实验表明:BPA-MIPs的吸附平衡时间约为60 min,最大吸附量为151.86μmol/g。结合流动注射-分子荧光法建立了一种选择性好、简单快速、平衡吸附时间短、吸附量大、结果准确可靠的测定水中双酚A的新方法。本方法,BPA溶液在20~100μg/L的范围内线性相关,相关系数R2=0.9985,相对标准偏差(20μg/L,n=5)为0.77%,检出限为0.17μg/L,回收率为97.2%~101.6%。
Molecularly imprinted polymers of bisphenol A(BPA-MIPs) were synthesized with precipitation polymerization techniques by using bisphenol A as the template, β-cyclodextrin as the functional monomers, dimethyl sulfoxide as solvent, hexamethylene diisocyanate as the crosslinking agent, and azobisisobutyronitrile as an initiator. The results indicated that the adsorbance of MIPs to BPA no longer increased after one hour and the maximum adsorption was 151.86 μmol/g. The BPA imprint polymers gave much higher affinity and selectivity for BPA. Using BPA-MIPs, a method using flow injection-molecular fluorescence spectrometry to detect BPA in water was set up. The linear correlation was better in the range of 20 to 100 μg/L, the correlation coefficient was 0.9985, the relative standard deviation(20 μg/L, n=5) was 0.77%, the detection limit was 0.17 μg/L, and recovery rate was from 97.2% to 101.6%.
Molecularly imprinted polymers of bisphenol A(BPA-MIPs) were synthesized with precipitation polymerization techniques by using bisphenol A as the template, β-cyclodextrin as the functional monomers, dimethyl sulfoxide as solvent, hexamethylene diisocyanate as the crosslinking agent, and azobisisobutyronitrile as an initiator. The results indicated that the adsorbance of MIPs to BPA no longer increased after one hour and the maximum adsorption was 151.86 μmol/g. The BPA imprint polymers gave much higher affinity and selectivity for BPA. Using BPA-MIPs, a method using flow injection-molecular fluorescence spectrometry to detect BPA in water was set up. The linear correlation was better in the range of 20 to 100 μg/L, the correlation coefficient was 0.9985, the relative standard deviation(20 μg/L, n=5) was 0.77%, the detection limit was 0.17 μg/L, and recovery rate was from 97.2% to 101.6%.
引文
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[2]胡文兰,刘建毅,张倩.中国卫生检验杂志,2011,21(2):530.
[3]Ye,L.;Weiss,R.;Mosbach,K.;Macromolecules,2000,33(22):8239.