热敏型分子印迹纳米传感器的构建及其荧光检测藻蓝蛋白
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- 英文论文题名：Thermosensitive Molecularly Imprinted Nanosensor for Fluorescent Detection of Phycocyanin
- 论文作者：傅骏青 ; 吴夏青 ; 王晓艳 ; 李金花 ; 陈令新
- 英文论文作者：Junqing Fu ; Xiaqing Wu ; Xiaoyan Wang ; Jinhua Li ; Lingxin Chen ; Yantai Institute of Coastal Zone Research ; Chinese Academy of Sciences ; College of Chemistry and Chemical Engineering ; Qufu Normal University
- 年：2016
- 作者机构：中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室;曲阜师范大学化学与化工学院山东省生命有机分析重点实验室;
- 论文关键词：藻蓝蛋白 ; 分子印迹 ; 热敏 ; 荧光检测 ; 海水
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十三分会：复杂样品分离分析
- 语种：中文
- 分类号：TP212;O657.3;X832
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十三分会 ; 复杂样品分离分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：224k
- 原文格式：D
- 会议级别：全国
摘要
藻蓝蛋白具有优异的荧光性能和重要的生理价值,常用作蓝藻爆发的指示蛋白~([1])。刺激响应分子印迹聚合物(SR-MIPs)己引起日益关注,可以在外界条件刺激下完成对目标分子的特异性控释~([2])。因此,我们以藻蓝蛋白为模板分子,通过自由基聚合,构建了一种热敏型SR-MIPs纳米传感器,借助藻蓝蛋白的自带荧光属性和TGA-GSH共修饰后的量子点对藻蓝蛋白进行高效特异识别和高灵敏荧光检测,如图1所示,通过控制不同的温度对模板蛋白进行选择性控释。对该传感器的温度响应、选择性、分析性能和应用等进行了系统研究。该传感器在0.01-1.8μM有良好线性关系,检测限10.3 nM,海水中回收率92.0-106.8%。该研究提供了一种智能的识别分析水样中痕量蛋白质的有效方法,丰富了蛋白质印迹和刺激响应印迹的相关研究。
Phycocyanin(PC) with excellent fluorescence characteristics and important physiological significance is an effective indicator for cyanobacterial bloom assessment due to its close relationship with cyanobacterial biomass[1].Stimuli-responsive molecularly imprinted polymers(SR-MIPs) have received increasing attentions due to their unique feature that the molecular recognition specificity is regulated by specific external stimuli[2].Herein,based on free radical polymerization,a novel thermosensitive imprinted nanosensor was fabricated using PC as template,for controlled adsorption/release of PC with temperature change,and its fluorescent detection.The SR-MIPs sensor was well characterized,and its temperature-responsive ability,binding property,recognition selectivity,analytical performances and practicality were investigated systematically.It presented a satisfactory linearity within 0.01-1.8μM,a low detection limit of 10.3 nM and high recoveries of 92.0-106.8%for spiked seawater samples.The study provided a facile,rapid and intelligent way for identification/analysis of trace proteins and enriched protein imprinting and stimuli-responsive imprinting related researches.
Phycocyanin(PC) with excellent fluorescence characteristics and important physiological significance is an effective indicator for cyanobacterial bloom assessment due to its close relationship with cyanobacterial biomass[1].Stimuli-responsive molecularly imprinted polymers(SR-MIPs) have received increasing attentions due to their unique feature that the molecular recognition specificity is regulated by specific external stimuli[2].Herein,based on free radical polymerization,a novel thermosensitive imprinted nanosensor was fabricated using PC as template,for controlled adsorption/release of PC with temperature change,and its fluorescent detection.The SR-MIPs sensor was well characterized,and its temperature-responsive ability,binding property,recognition selectivity,analytical performances and practicality were investigated systematically.It presented a satisfactory linearity within 0.01-1.8μM,a low detection limit of 10.3 nM and high recoveries of 92.0-106.8%for spiked seawater samples.The study provided a facile,rapid and intelligent way for identification/analysis of trace proteins and enriched protein imprinting and stimuli-responsive imprinting related researches.
引文
[1]Wang,X.;Yu,J.;Kang,Q.;Shen,D;Li,J.;Chen,L.Biosens.Bioelectron.2016,77:624
[2]Chen,L.;Wang,X.;Lu,W.;Wu,X.;Li,J.Chem.Soc.Rev.2016,DOI:10.1039/c6cs00061d.
[2]Chen,L.;Wang,X.;Lu,W.;Wu,X.;Li,J.Chem.Soc.Rev.2016,DOI:10.1039/c6cs00061d.