温敏性蛋白分子印迹聚合物的制备及其性能研究
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- 英文论文题名：Poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for specific recognition and release of lysozyme
- 论文作者：吉时蕾 ; 李楠 ; 乔娟 ; 齐莉 ; 陈义
- 英文论文作者：Shilei Ji ; Nan Li ; Juan Qiao ; Li Qi ; Yi Chen ; Key Laboratory of Analytical Chemistry for Living Biosystems ; Institute of Chemistry ; Chinese Academy of Sciences
- 年：2016
- 作者机构：中国科学院化学研究所,活体分析化学院重点实验室;
- 论文关键词：聚氨基酸 ; 温敏性分子印迹聚合物 ; 磁性纳米颗粒 ; 溶菌酶
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十三分会：复杂样品分离分析
- 语种：中文
- 分类号：O631.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十三分会 ; 复杂样品分离分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：115k
- 原文格式：D
- 会议级别：全国
摘要
采用表面分子印迹技术,以溶菌酶为模板分子,以磁性纳米颗粒为基质,以聚氨基酸为主要功能单体,以N,N'-亚甲基双丙烯酰胺为交联剂,制备了对溶菌酶具有特异性识别的温敏性分子印迹聚合物@磁性纳米颗粒材料。考察了其温敏性及其对溶菌酶的吸附性能;探讨了其吸附动力学、吸附热力学及选择性;并进一步将其用于溶菌酶的磁性分离富集研究中。研究结果表明:所制备的温敏性分子印迹聚合物具有较好的温敏性,在一定温度范围内,仅通过改变环境温度,就能实现对溶菌酶分子的特异性捕捉与释放;对溶菌酶具有较高的吸附容量(103.09 mg g~(-1))、吸附平衡快速(15 min)及良好的选择性;能实现对蛋清样品中的溶菌酶的分离与富集。此外,再外加磁场作用下,所制备的温敏性分子印迹聚合物能快速与基质分离,不仅提高了研究工作效率,也人们为从复杂生物样品中分离富集溶菌酶提供了一种聚合物基质新材料。
Poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for recognition and release of lysozyme was prepared via surface imprinting method.For constructing the molecularly imprinted polymer(MIP) layer,amino acid-based thermoresponsive monomer(Nmethacryloyl-L-alanine methyl ester,MA-L-Ala-OMe) was selected for the functional monomer.The dynamic light scattering studies and swelling ratios measurements were carried out for demonstrating the thermoresponsive property of the imprinted nanoparticles.The prepared magnetic MIP nanoparticles showed good adsorption capacity and selective recognition properties to lysozyme.Moreover,the fast adsorption process could reach equilibrium within 15 min.Importantly,the capture and release of lysozyme could be easily realized simply by altering the temperature of aqueous solution.Furthermore,the prepared imprinted nanoparticles were applied to separate lysozyme from the real egg white samples.The results proved that the thermoresponsive MIPs based on MA-L-Ala-OMe have great potential for selectively enriching target proteins in real samples.
Poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for recognition and release of lysozyme was prepared via surface imprinting method.For constructing the molecularly imprinted polymer(MIP) layer,amino acid-based thermoresponsive monomer(Nmethacryloyl-L-alanine methyl ester,MA-L-Ala-OMe) was selected for the functional monomer.The dynamic light scattering studies and swelling ratios measurements were carried out for demonstrating the thermoresponsive property of the imprinted nanoparticles.The prepared magnetic MIP nanoparticles showed good adsorption capacity and selective recognition properties to lysozyme.Moreover,the fast adsorption process could reach equilibrium within 15 min.Importantly,the capture and release of lysozyme could be easily realized simply by altering the temperature of aqueous solution.Furthermore,the prepared imprinted nanoparticles were applied to separate lysozyme from the real egg white samples.The results proved that the thermoresponsive MIPs based on MA-L-Ala-OMe have great potential for selectively enriching target proteins in real samples.
引文
[1]Ji.S.L.;Li N.;Shen Y.;Qi,L.*;Qiao J.;Li Z.B.Anal.Chim.Acta,2016,909,60-66.
[2]Li,N.;Qi,L.*;Shen,Y.;Qiao,J.;Chen,Y.ACS Appl.Mater.Interfaces,2014,19,17289-17295.
[2]Li,N.;Qi,L.*;Shen,Y.;Qiao,J.;Chen,Y.ACS Appl.Mater.Interfaces,2014,19,17289-17295.