板栗多糖-硒纳米粒子复合物的制备、表征及抗肿瘤活性研究
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- 英文论文题名：Synthesis,characterization and antitumor activity of Castanea mollissima Blume polysaccharide stabilized selenium nanoparticles
- 论文作者：李红燕 ; 王应杏 ; 李胜辉 ; 王书香 ; 张金超
- 英文论文作者：Hongyan Li ; Yingxing Wang ; Shenghui Li ; Shuxiang Wang ; Jinchao Zhang ; College of Chemistry & Environmental Science ; Hebei University ; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education
- 年：2016
- 作者机构：河北大学,化学与环境科学学院;药物化学与分子诊断教育部重点实验室;
- 论文关键词：板栗 ; 葡聚糖 ; 硒纳米粒子 ; 抗肿瘤 ; 凋亡
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十八分会：化学生物学
- 语种：中文
- 分类号：TB383.1;R943;R96
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十八分会 ; 化学生物学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：212k
- 原文格式：D
- 会议级别：全国
摘要
探讨板栗多糖纳米硒的制备及其在体外对宫颈癌HeLa细胞生长和凋亡的作用机制。以板栗多糖为软模板制备多糖纳米硒并进行化学表征,体外以宫颈癌HeLa细胞为研究对象,MTT法检测板栗多糖及其纳米硒复合物对肿瘤细胞的抑制作用,流式细胞仪检测细胞周期、细胞凋亡、活性氧水平、线粒体膜电位和Caspase-3蛋白的表达。从板栗种仁中提取得到水溶性的α-D-1,6-葡聚糖(CPA),其分子量约为2×10~3kDa。以不同浓度的CPA为模板(0.1,1,5,10 mg/mL),室温采用抗坏血酸还原亚硒酸钠的方法制备多糖纳米硒(CPA-SeNPs)。TEM检测发现CPA为5.0mg/mL时,多糖纳米硒的水溶性和分散性较好,其粒径为50 nm。ICP-AES测得硒含量为171.4 mg/g。体外肿瘤细胞实验证明,CPA-SeNPs较CPA对HeLa细胞具有较强的增殖抑制作用,可将细胞周期阻滞于G2/M期并可诱导HeLa细胞凋亡。此外,CPA-SeNPs还可通过升高细胞内活性氧水平,降低线粒体膜电位和激活Caspase-3诱导HeLa细胞发生凋亡。板栗多糖纳米硒较板栗多糖对HeLa细胞具有较强的增殖抑制作用,这对宫颈癌的治疗具有潜在的应用价值。
A water soluble polysaccharide(CPA) was isolated from Castanea mollissima Blume,CPA was a a-D-l,6-glucan with a high molecular weight of about 2×10~3 kDa.CPA-SeNPs was prepared with different concentration of CPA and Na_2SeO_3 reduced with ascorbic acid.When the concentration of CPA was 5.0 mg/mL,the nanoparticle was water soluble and dispersed with the particle diameters of 50 nm.The Se content in CPA-SeNPs was 171.4 mg/g.CPA-SeNPs showed a significant antiproliferative effect on HeLa cells than CPA in vitro.CPA and CPA-SeNPs could arrest HeLa cells in G2/M phase and induce HeLa cells apoptosis.While the CPA-SeNPs apoptotic pathway was also characterized by the reactive oxygen species(ROS) overproduction,mitochondrial dysfunction and activation of caspase-3.This study suggested that CPA-SeNPs can be developed as a candidate for cancer treatment and prevention,especially human cervical carcinoma cells.
A water soluble polysaccharide(CPA) was isolated from Castanea mollissima Blume,CPA was a a-D-l,6-glucan with a high molecular weight of about 2×10~3 kDa.CPA-SeNPs was prepared with different concentration of CPA and Na_2SeO_3 reduced with ascorbic acid.When the concentration of CPA was 5.0 mg/mL,the nanoparticle was water soluble and dispersed with the particle diameters of 50 nm.The Se content in CPA-SeNPs was 171.4 mg/g.CPA-SeNPs showed a significant antiproliferative effect on HeLa cells than CPA in vitro.CPA and CPA-SeNPs could arrest HeLa cells in G2/M phase and induce HeLa cells apoptosis.While the CPA-SeNPs apoptotic pathway was also characterized by the reactive oxygen species(ROS) overproduction,mitochondrial dysfunction and activation of caspase-3.This study suggested that CPA-SeNPs can be developed as a candidate for cancer treatment and prevention,especially human cervical carcinoma cells.
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