灵芝超临界萃取物通过内质网实现的光老化抑制效果
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- 英文论文题名：Photoaging-suppressing effect mediated with endoplasmic reticulum of Reishi supercritical fluid extracts
- 论文作者：山羽宏行 ; 坂井田勉 ; 田中浩 ; 八代洋一 ; 中田悟
- 英文论文作者：Hiroyuki Yamaba ; Tsutomu Sakaida ; Hiroshi Tanaka ; Youichi Yashiro ; Satoru Nakata ; Research Laboratories ; Nippon Menard Cosmetic Co. ; Ltd.
- 年：2016
- 作者机构：日本美伊娜多化妆品株式会社综合研究所;
- 论文关键词：光老化 ; 紫灵芝 ; 内质网 ; 紫外线 ; 胶原 ; 运输小泡
- 英文论文关键词：photoaging ; Ganoderma sinense ; endoplasmic reticulum ; ultraviolet ; collagen ; transport vesicle
- 会议召开时间：2016-06-15
- 会议录名称：第十一届中国化妆品学术研讨会论文集
- 英文会议录名称：The Proceedings of the 11th Seminar on Cosmetic China
- 语种：中文
- 分类号：TQ658.2
- 学会代码：ZGXE
- 会议名称：第十一届中国化妆品学术研讨会
- 会议地点：中国上海
- 主办单位：中国香料香精化妆品工业协会
- 学会名称：中国香料香精化妆品工业协会
- 页数：7
- 文件大小：1480k
- 原文格式：O
- 会议级别：全国
摘要
长期暴露于日照下的皮肤会长出很深的皱纹,这是皮肤光老化的显著特征,受生理衰老和紫外线(UV)的双重影响。虽然该现象与成纤维细胞中胶原生成不足有很大关系,其具体机制尚不清楚。紫灵芝(Ganoderma sinense)是灵芝属Ganodermataceae真菌,一直被用作抗衰老的天然药材。本研究围绕灵芝超临界萃取物的抑制光老化效果,聚焦于生成胶原的细胞内质网(ER)。结果显示,UV照射减缓始于ER的蛋白运输,蛋白异常聚积造成ER肿胀,该ER机能障碍从而被判断为光老化皮肤中胶原不足的主要原因之一。灵芝超临界萃取物能通过优化调控蛋白运输改善UV造成的ER功能障碍性胶原生成不足,因此是抑制光老化的有效成份。
Chronically sun-exposed skin has the characteristic features including a deep wrinkled appearance that is the hallmark of photoaging skin,in which age-related changes in combination with the influence of ultraviolet(UV) rays become advanced.Although these phenomena are related to the reduction of collagen production in skin fibroblasts,detailed mechanisms are not clear.Reishi(Ganoderma sinense) is a Ganodermataceae mushroom used as a crude drug for anti-aging.In this study,we investigated photoaging-suppressing effect of Reishi supercritical fluid extracts focused on a cell organelle,endoplasmic reticulum(ER) in which collagen is produced.As a result,UV irradiation decreased protein transports from ER,followed by ER enlargement owing to abnormal protein accumulation.This ER dysfunction is considered to be one of the mechanisms responsible for collagen reductions observed in photoaging skin because it caused the decrease of collagen production.Reishi supercritical fluid extracts could improve UV-induced collagen reduction due to ER dysfunction by upregulation of protein transports.Therefore it was considered to be an available ingredient contributing to the suppression of photoaging.
Chronically sun-exposed skin has the characteristic features including a deep wrinkled appearance that is the hallmark of photoaging skin,in which age-related changes in combination with the influence of ultraviolet(UV) rays become advanced.Although these phenomena are related to the reduction of collagen production in skin fibroblasts,detailed mechanisms are not clear.Reishi(Ganoderma sinense) is a Ganodermataceae mushroom used as a crude drug for anti-aging.In this study,we investigated photoaging-suppressing effect of Reishi supercritical fluid extracts focused on a cell organelle,endoplasmic reticulum(ER) in which collagen is produced.As a result,UV irradiation decreased protein transports from ER,followed by ER enlargement owing to abnormal protein accumulation.This ER dysfunction is considered to be one of the mechanisms responsible for collagen reductions observed in photoaging skin because it caused the decrease of collagen production.Reishi supercritical fluid extracts could improve UV-induced collagen reduction due to ER dysfunction by upregulation of protein transports.Therefore it was considered to be an available ingredient contributing to the suppression of photoaging.
引文
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[2]Berneburg M,Plettenberg H,Krutmann J.Photoaging of human skin[J].Photodermatol.Photoimmunol.Photomed.,2000,16:239-244.
[3]Quan T,Qin Z,Xia W etc.Matrix-degrading metalloproteinases in photoaging[J].J.Investig.Dermatol.Symp.Proa,2009,14:20-24.
[4]Fisher G J,Datta S C,Talwar H S etc.Molecular basis of sun-induced premature skin ageing and retinoid antagonism[J].Nature,1996,379:335-9.
[5]Fisher G J,Wang Z Q,Datta S C etc.Pathophysiology of premature skin aging induced by ultraviolet light[J].N.Engl.J.Med.,1997,337:1419-28.
[6]Tanaka H,Yamaba H,Kosugi N etc.Fermentable metabolite of Zymomonas mobilis controls collagen reduction in photoaging skin by improving TGF-beta/Smad signaling suppression[J].Arch.Dermatol.Res.,2008,300:S57-64.
[7]#12
[8]Murakami T,Saito A,Hino S etc.Signaling mediated by the endoplasmic reticulum stress transducer OASIS is involved in bone formation[J].Nat.Cell.Biol.,2009,11:1205-1211.
[9]Barlowe C,Orci L,Yeung T etc.COPII:a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum[J].Cell,1994,77:895-907.
[10]Sato K,Nakano A.Mechanisms of COPII vesicle formation and protein sorting[J].FEBS Lett.,2007,581:2076-2082.
[11]Stephens D J,Pepperkok R,Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells[J].J.Cell Sci.,2002,115:1149-1160.
[12]Sarmah S,Barrallo-Gimeno A,Melville D B etc.Sec24D-dependent transport of extracellular matrix proteins is required for zebrafish skeletal morphogenesis[J].PLoS One,2010,5:e10367.
[13]Johno H,Nakajima S,Kato H etc.Unfolded protein response causes a phenotypic shift of inflamed glomerular cells towardredifferentiation through dual blockade of Akt and Smad signaling pathways[J].Am.J.Pathol.,2012,181:1977-1990.