Effects of Quaternized Chitosan/Alginate-Fe_3O_4 Magnetic Nanoparticles on Enhancing Chemosensitization of Multidrug-resistant Gastric Carcinoma by Regulating Cell Autophagy Activity in Mice
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- 英文论文题名：Effects of Quaternized Chitosan/Alginate-Fe_3O_4 Magnetic Nanoparticles on Enhancing Chemosensitization of Multidrug-resistant Gastric Carcinoma by Regulating Cell Autophagy Activity in Mice
- 论文作者：Jing Feng ; Xiujuan Li ; Ran Zhang ; Tao Su ; Zuhong Tian ; Dong Han ; Chuanxu Zhao ; Xuyang Feng ; Yongzhan Nie ; Kaichun Wu ; Yundai Chen ; Hongbing Deng ; Feng Cao
- 英文论文作者：Jing Feng ; Xiujuan Li ; Ran Zhang ; Tao Su ; Zuhong Tian ; Dong Han ; Chuanxu Zhao ; Xuyang Feng ; Yongzhan Nie ; Kaichun Wu ; Yundai Chen ; Hongbing Deng ; Feng Cao ; Department of Emergency Medicine ; Jinling Hospital ; Department of Cardiology ; Xijing Hospital ; Fourth Military Medical University ; Department of Cardiology ; Chinese PLA General Hospital ; Department of Gastroenterology ; Xijing Hospital ; Fourth Military Medical University ; School of Resource and Environmental Science ; Wuhan University
- 年：2016
- 作者机构：Department of Emergency Medicine,Jinling Hospital;Department of Cardiology,Xijing Hospital,Fourth Military Medical University;Department of Cardiology,Chinese PLA General Hospital;Department of Gastroenterology,Xijing Hospital,Fourth Military Medical University;School of Resource and Environmental Science,Wuhan University;
- 英文论文关键词：HTCC-MNPs ; multidrug resistant(MDR) gastric cancer ; autophagy ; mitochondrial function ; reactive oxygen species(ROS)
- 会议召开时间：2016-06-17
- 会议录名称：2016中国中毒救治首都论坛——暨第八届全国中毒及危重症救治学术会议论文集
- 语种：英文
- 分类号：R735.2
- 学会代码：ZGDV
- 会议名称：2016中国中毒救治首都论坛——暨第八届全国中毒及危重症救治学术会议
- 会议地点：中国北京
- 主办单位：中国毒理学会中毒与救治专业委员会
- 学会名称：中国毒理学会
- 页数：9
- 文件大小：862k
- 原文格式：O
- 会议级别：全国
摘要
Multidrug resistance(MDR) and targeted therapies remain as vital challenges in tumor chemotherapy.Nanoparticles provide promising application for cancer theranostics as for their excellent advantages of tumor-targeted cytotoxicity and imaging capability.In present study,we developed N-((2-hydroxy-3-trimethylammonium) propyl) chitosan chloride(HTCC)/alginate encapsulated Fe_3O_4 magnetic nanoparticles(HTCC-MNPs) and applied it into MDR gastric cancer both in vivo and in vitro.HTCC-MNPs were fabricated using ALG,Fe_3O_4 and HTCC in ionic gelation method.The size and physical characteristics of nanoparticles were detected by dynamic light scattering,transmission electron microscopy(TEM) and zeta potential analysis.HTCC-MNPs exhibited excellent water solubility and biocompatibility and significantly reduced cell viability in drug-resistant cancer cell line SGC7901/ADR cells but not in normal gastric cells(P < 0.05).LC3 analysis demonstrated the involvement of autophagy in HTCC-MNPs cytotoxicity.Additionally,apoptosis was verified by DNA content assay.HTCC-MNPs led to mitochondrial membrane potential loss,decreased ATP production and excessive reactive oxygen species(ROS) generation as compared with control group(P < 0.05).Magnetic resonance imaging showed the enrichment of HTCC-MNPs in tumor-bearing mice.In vivo.Bioluminensence imaging and tumor volume measurement revealed that HTCC-MNPs markedly inhibited in vivo tumor growth(P < 0.05).In conclusion,HTCC-MNPs significantly inhibited MDR gastric tumor growth and decreased tumor volume via the induction of cellular autophagy and apoptosis,which was attributed to mitochondrial dysfunction and excessive ROS accumulation.
Multidrug resistance(MDR) and targeted therapies remain as vital challenges in tumor chemotherapy.Nanoparticles provide promising application for cancer theranostics as for their excellent advantages of tumor-targeted cytotoxicity and imaging capability.In present study,we developed N-((2-hydroxy-3-trimethylammonium) propyl) chitosan chloride(HTCC)/alginate encapsulated Fe_3O_4 magnetic nanoparticles(HTCC-MNPs) and applied it into MDR gastric cancer both in vivo and in vitro.HTCC-MNPs were fabricated using ALG,Fe_3O_4 and HTCC in ionic gelation method.The size and physical characteristics of nanoparticles were detected by dynamic light scattering,transmission electron microscopy(TEM) and zeta potential analysis.HTCC-MNPs exhibited excellent water solubility and biocompatibility and significantly reduced cell viability in drug-resistant cancer cell line SGC7901/ADR cells but not in normal gastric cells(P < 0.05).LC3 analysis demonstrated the involvement of autophagy in HTCC-MNPs cytotoxicity.Additionally,apoptosis was verified by DNA content assay.HTCC-MNPs led to mitochondrial membrane potential loss,decreased ATP production and excessive reactive oxygen species(ROS) generation as compared with control group(P < 0.05).Magnetic resonance imaging showed the enrichment of HTCC-MNPs in tumor-bearing mice.In vivo.Bioluminensence imaging and tumor volume measurement revealed that HTCC-MNPs markedly inhibited in vivo tumor growth(P < 0.05).In conclusion,HTCC-MNPs significantly inhibited MDR gastric tumor growth and decreased tumor volume via the induction of cellular autophagy and apoptosis,which was attributed to mitochondrial dysfunction and excessive ROS accumulation.
Multidrug resistance(MDR) and targeted therapies remain as vital challenges in tumor chemotherapy.Nanoparticles provide promising application for cancer theranostics as for their excellent advantages of tumor-targeted cytotoxicity and imaging capability.In present study,we developed N-((2-hydroxy-3-trimethylammonium) propyl) chitosan chloride(HTCC)/alginate encapsulated Fe_3O_4 magnetic nanoparticles(HTCC-MNPs) and applied it into MDR gastric cancer both in vivo and in vitro.HTCC-MNPs were fabricated using ALG,Fe_3O_4 and HTCC in ionic gelation method.The size and physical characteristics of nanoparticles were detected by dynamic light scattering,transmission electron microscopy(TEM) and zeta potential analysis.HTCC-MNPs exhibited excellent water solubility and biocompatibility and significantly reduced cell viability in drug-resistant cancer cell line SGC7901/ADR cells but not in normal gastric cells(P < 0.05).LC3 analysis demonstrated the involvement of autophagy in HTCC-MNPs cytotoxicity.Additionally,apoptosis was verified by DNA content assay.HTCC-MNPs led to mitochondrial membrane potential loss,decreased ATP production and excessive reactive oxygen species(ROS) generation as compared with control group(P < 0.05).Magnetic resonance imaging showed the enrichment of HTCC-MNPs in tumor-bearing mice.In vivo.Bioluminensence imaging and tumor volume measurement revealed that HTCC-MNPs markedly inhibited in vivo tumor growth(P < 0.05).In conclusion,HTCC-MNPs significantly inhibited MDR gastric tumor growth and decreased tumor volume via the induction of cellular autophagy and apoptosis,which was attributed to mitochondrial dysfunction and excessive ROS accumulation.
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