Decidual stromal cells(DSC) promote the phenotypic convention from peripheral blood natural killer(pNK) cells to decidual natural killer(dNK) cells via a disintegrin and metalloprotease-17(ADAM17)
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- 英文论文题名：Decidual stromal cells(DSC) promote the phenotypic convention from peripheral blood natural killer(pNK) cells to decidual natural killer(dNK) cells via a disintegrin and metalloprotease-17(ADAM17)
- 论文作者：Ya Sun ; Li Mingqing ; He Yinyan
- 英文论文作者：Ya Sun ; Li Mingqing ; He Yinyan ; Department of Obstetrics & Gynecology ; Shanghai General Hospital ; School of Medicine ; Shanghai Jiao Tong University School of Medicine ; Laboratory for Reproductive Immunology ; Hospital and Institute of Obstetrics and Gynecology ; Fudan University
- 年：2016
- 作者机构：Department of Obstetrics & Gynecology,Shanghai General Hospital,School of Medicine,Shanghai Jiao Tong University School of Medicine;Laboratory for Reproductive Immunology,Hospital and Institute of Obstetrics and Gynecology,Fudan University;
- 英文论文关键词：dNK ; pNK ; CD16 ; DSC ; ADAM-17
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会壁报交流集
- 英文会议录名称：Proceedings of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R392
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：1046k
- 原文格式：D
- 会议级别：全国
摘要
Objective:d NK cells are generally considered to have a cytokine-secreting role rather than cytotoxic defence role of p NK cells.DSC,however,could recruit p NK cells to the decidua.We wondered whether DSC could change p NK cells phenotype towards d NK cells.Methods:We used FCM to test the expression of CD16 and ADAM-17 on p NK or d NK cells and DSC,respectively.ELISA kit tested the soluble CD16 in the coculture(p NK cells with DSC) supertanant or with ADAM-17 inhibitor,p NK conditioned medium or with ADAM-17 inhibitor.Results:(1)CD16+ d NK cells proportion was higher in abortional decidua than in normal.(2)ADAM-17+DSC proportion was lower in abortional decidua than in normal.(3)soluble CD16 concentration was higher in the coculture supertanant than in p NK and or with ADAM-17 inhibitor.(4)DSC regulated the shedding of CD16 on human p NK cells via ADAM-17.Conclusion:DSC may exert profound effect on the convention from pN K cells to dN K cells for a successfully pregnancy.
Objective:d NK cells are generally considered to have a cytokine-secreting role rather than cytotoxic defence role of p NK cells.DSC,however,could recruit p NK cells to the decidua.We wondered whether DSC could change p NK cells phenotype towards d NK cells.Methods:We used FCM to test the expression of CD16 and ADAM-17 on p NK or d NK cells and DSC,respectively.ELISA kit tested the soluble CD16 in the coculture(p NK cells with DSC) supertanant or with ADAM-17 inhibitor,p NK conditioned medium or with ADAM-17 inhibitor.Results:(1)CD16+ d NK cells proportion was higher in abortional decidua than in normal.(2)ADAM-17+DSC proportion was lower in abortional decidua than in normal.(3)soluble CD16 concentration was higher in the coculture supertanant than in p NK and or with ADAM-17 inhibitor.(4)DSC regulated the shedding of CD16 on human p NK cells via ADAM-17.Conclusion:DSC may exert profound effect on the convention from pN K cells to dN K cells for a successfully pregnancy.
Objective:d NK cells are generally considered to have a cytokine-secreting role rather than cytotoxic defence role of p NK cells.DSC,however,could recruit p NK cells to the decidua.We wondered whether DSC could change p NK cells phenotype towards d NK cells.Methods:We used FCM to test the expression of CD16 and ADAM-17 on p NK or d NK cells and DSC,respectively.ELISA kit tested the soluble CD16 in the coculture(p NK cells with DSC) supertanant or with ADAM-17 inhibitor,p NK conditioned medium or with ADAM-17 inhibitor.Results:(1)CD16+ d NK cells proportion was higher in abortional decidua than in normal.(2)ADAM-17+DSC proportion was lower in abortional decidua than in normal.(3)soluble CD16 concentration was higher in the coculture supertanant than in p NK and or with ADAM-17 inhibitor.(4)DSC regulated the shedding of CD16 on human p NK cells via ADAM-17.Conclusion:DSC may exert profound effect on the convention from pN K cells to dN K cells for a successfully pregnancy.
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