线粒体内谷胱甘肽比例荧光成像
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摘要
线粒体作为一种重要细胞器,它不但为细胞代谢提供能量,而且还可调控细胞生长周期。但线粒体在其功能实施过程中会产生大量对人体有害的自由基,并损伤生物膜,侵袭生命大分子,加快机体衰老,并诱发各种疾病,例如动脉粥样硬化、肿瘤等。~([1,2])而线粒体内谷胱甘肽(GSH)能够清除掉线粒体产生的自由基,减弱或消除线粒体在功能实施过程中对生物体的损伤。~([3])因此,对线粒体内GSH的实时定量检测和原位成像将有助于细胞器功能以及脉粥样硬化、肿瘤等疾病的基础研究。本研究中以萘的衍生物为母体,设计合成了特异性识别GSH的荧光探针分子。后期测试结果显示,探针分子可以用来对线粒体内的GSH进行实时原位的比率检测和定位成像(Figure1),且其具有良好的溶解性,低的细胞毒性(Figure2)和光毒性。
Mitochondria not only provide energy for cellular metabolism, but also regulate cell growth cycle.~([1,2]) However, mitochondria would produce a large amount of harmful free radicals in the implementation process of its functions, which could lead to a number of diseases, such as neurodegenerative diseases, obesity, cancer, and so on.~([3]) Glutathione(GSH) in cell, especially in the mitochondria, could remove free radicals produced by mitochondria, which avoid the occurrence of atherosclerosis, cancer and other diseases. Therefore, real-time quantitative detecting and imaging would help the function study of organelles and diseases research. In this paper, the probe for GSH was reported. The probe with suitable solubility, low cytotoxicity and low light toxicity was applied to in situ real-time ratio study the related disease about glutathione/mitochondria.
引文
1.Shao,N.;Jin,J.;Wang,H.;Zheng,J.;Yang,R.;Chan,W.;Abliz,C.J.Am.Chem.Soc.2010,132,725.
    2.Yin,J.;Kwon,Y.;Kim,D.;Lee,D.;Kim,G.;Hu,Y.;Ryu,J.H.;Yoon,J.J.Am.Chem.Soc.2014,136,5351.
    3.Niu,L.Y.;Guan,Y.S.;Chen,Y.Z.;Wu,L.Z.;Tung,C.H.;Yang,Q.Z.J.Am.Chem.Soc.2012,134,18928.

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