线粒体内谷胱甘肽比例荧光成像
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- 英文论文题名：The Ratio Fluorescent Imaging of Glutathione in the Mitochondria
- 论文作者：王彩霞 ; 牛慧玉 ; 仉华 ; 郭海明
- 英文论文作者：Caixia Wang ; Huiyu Niu ; Hua Zhang ; Haiming Guo ; School of Chemistry and Chemical Engineering ; Henan Normal University
- 年：2016
- 作者机构：河南师范大学化学化工学院绿色化学介质与反应教育部重点实验室;
- 论文关键词：比例成像 ; 谷胱甘肽 ; 线粒体
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十八分会：化学生物学
- 语种：中文
- 分类号：Q26
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十八分会 ; 化学生物学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：234k
- 原文格式：D
- 会议级别：全国
摘要
线粒体作为一种重要细胞器,它不但为细胞代谢提供能量,而且还可调控细胞生长周期。但线粒体在其功能实施过程中会产生大量对人体有害的自由基,并损伤生物膜,侵袭生命大分子,加快机体衰老,并诱发各种疾病,例如动脉粥样硬化、肿瘤等。~([1,2])而线粒体内谷胱甘肽(GSH)能够清除掉线粒体产生的自由基,减弱或消除线粒体在功能实施过程中对生物体的损伤。~([3])因此,对线粒体内GSH的实时定量检测和原位成像将有助于细胞器功能以及脉粥样硬化、肿瘤等疾病的基础研究。本研究中以萘的衍生物为母体,设计合成了特异性识别GSH的荧光探针分子。后期测试结果显示,探针分子可以用来对线粒体内的GSH进行实时原位的比率检测和定位成像(Figure1),且其具有良好的溶解性,低的细胞毒性(Figure2)和光毒性。
Mitochondria not only provide energy for cellular metabolism, but also regulate cell growth cycle.~([1,2]) However, mitochondria would produce a large amount of harmful free radicals in the implementation process of its functions, which could lead to a number of diseases, such as neurodegenerative diseases, obesity, cancer, and so on.~([3]) Glutathione(GSH) in cell, especially in the mitochondria, could remove free radicals produced by mitochondria, which avoid the occurrence of atherosclerosis, cancer and other diseases. Therefore, real-time quantitative detecting and imaging would help the function study of organelles and diseases research. In this paper, the probe for GSH was reported. The probe with suitable solubility, low cytotoxicity and low light toxicity was applied to in situ real-time ratio study the related disease about glutathione/mitochondria.
Mitochondria not only provide energy for cellular metabolism, but also regulate cell growth cycle.~([1,2]) However, mitochondria would produce a large amount of harmful free radicals in the implementation process of its functions, which could lead to a number of diseases, such as neurodegenerative diseases, obesity, cancer, and so on.~([3]) Glutathione(GSH) in cell, especially in the mitochondria, could remove free radicals produced by mitochondria, which avoid the occurrence of atherosclerosis, cancer and other diseases. Therefore, real-time quantitative detecting and imaging would help the function study of organelles and diseases research. In this paper, the probe for GSH was reported. The probe with suitable solubility, low cytotoxicity and low light toxicity was applied to in situ real-time ratio study the related disease about glutathione/mitochondria.
引文
1.Shao,N.;Jin,J.;Wang,H.;Zheng,J.;Yang,R.;Chan,W.;Abliz,C.J.Am.Chem.Soc.2010,132,725.
2.Yin,J.;Kwon,Y.;Kim,D.;Lee,D.;Kim,G.;Hu,Y.;Ryu,J.H.;Yoon,J.J.Am.Chem.Soc.2014,136,5351.
3.Niu,L.Y.;Guan,Y.S.;Chen,Y.Z.;Wu,L.Z.;Tung,C.H.;Yang,Q.Z.J.Am.Chem.Soc.2012,134,18928.
2.Yin,J.;Kwon,Y.;Kim,D.;Lee,D.;Kim,G.;Hu,Y.;Ryu,J.H.;Yoon,J.J.Am.Chem.Soc.2014,136,5351.
3.Niu,L.Y.;Guan,Y.S.;Chen,Y.Z.;Wu,L.Z.;Tung,C.H.;Yang,Q.Z.J.Am.Chem.Soc.2012,134,18928.