The key role of NF-YC genes in crosstalk between GA and ABA during Arabidopsis seed germination
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摘要
The antagonistic crosstalk between gibberellic acid(GA) and abscisic acid(ABA) plays a pivotal role in modulation of seed germination. However,the molecular mechanism of such phytohormone interaction remains largely elusive. The NUCLEAR FACTOR-Y C proteins(NF-YCs),structurally characterized by a histone-fold domain(HFD) and closely related to the core histone H2 A,functionally act as one subunit of the NF-Y heterotrimer transcriptional factor that specifically recognizes the CCAAT-box in eukaryotes. Here we show that three Arabidopsis NF-YC homologues NF-YC3,NFYC4,and NF-YC9 redundantly modulate GA-and ABA-mediated seed germination,contributing to the integration of GA and ABA signaling through directly interacting with RGA-LIKE 2(RGL2),a key repressor of GA signaling. The NF-YC-RGL2 module targets ABA INSENSITIVE 5(ABI5),the gene encoding a core component of ABA signaling,via specific CCAAT elements and collectively regulates a set of GA-and ABA-responsive genes,thus control germination. These results illustrate a novel regulatory model that reveals a central molecular link of NF-YC-RGL2-ABI5 in integration of GA and ABA signaling pathways during seed germination,providing a significant mechanistic understanding on how interaction of GA and ABA signaling is fine-tuned in seed germination.
The antagonistic crosstalk between gibberellic acid(GA) and abscisic acid(ABA) plays a pivotal role in modulation of seed germination. However,the molecular mechanism of such phytohormone interaction remains largely elusive. The NUCLEAR FACTOR-Y C proteins(NF-YCs),structurally characterized by a histone-fold domain(HFD) and closely related to the core histone H2 A,functionally act as one subunit of the NF-Y heterotrimer transcriptional factor that specifically recognizes the CCAAT-box in eukaryotes. Here we show that three Arabidopsis NF-YC homologues NF-YC3,NFYC4,and NF-YC9 redundantly modulate GA-and ABA-mediated seed germination,contributing to the integration of GA and ABA signaling through directly interacting with RGA-LIKE 2(RGL2),a key repressor of GA signaling. The NF-YC-RGL2 module targets ABA INSENSITIVE 5(ABI5),the gene encoding a core component of ABA signaling,via specific CCAAT elements and collectively regulates a set of GA-and ABA-responsive genes,thus control germination. These results illustrate a novel regulatory model that reveals a central molecular link of NF-YC-RGL2-ABI5 in integration of GA and ABA signaling pathways during seed germination,providing a significant mechanistic understanding on how interaction of GA and ABA signaling is fine-tuned in seed germination.
引文

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