Cell cycle re-entry relates to neuronal apoptosis after exposure to benzo[a]pyrene in SD rats
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- 英文论文题名：Cell cycle re-entry relates to neuronal apoptosis after exposure to benzo[a]pyrene in SD rats
- 论文作者：NIE Jisheng ; YAN Zhiwei ; DUAN Lei ; TANG Deliang ; NIU Qiao
- 英文论文作者：NIE Jisheng ; YAN Zhiwei ; DUAN Lei ; TANG Deliang ; NIU Qiao ; Department of Occupational Health ; School of Public Health ; Shanxi Medical University ; Department of Environmental Health Sciences and Columbia Center for Children's Environmental Health ; Mailman School of Public Health ; Columbia University
- 年：2016
- 作者机构：Department of Occupational Health,School of Public Health,Shanxi Medical University;Department of Environmental Health Sciences and Columbia Center for Children's Environmental Health,Mailman School of Public Health,Columbia University;
- 英文论文关键词：Benzo[a]pyrene ; neuronal apoptosis ; cell cycle re-entry
- 会议召开时间：2016-11-16
- 会议录名称：第六届中国毒理学会免疫毒理专业委员会换届及学术会议资料汇编
- 语种：英文
- 分类号：R114
- 学会代码：ZGDV
- 会议名称：第六届中国毒理学会免疫毒理专业委员会换届及学术会议
- 会议地点：中国河南开封
- 主办单位：中国毒理学会免疫毒理专业委员会
- 学会名称：中国毒理学会
- 页数：11
- 文件大小：2543k
- 原文格式：O
- 会议级别：全国
摘要
As a widely distributed environmental contaminant,B[a]P could cause neuronal cell apoptosis in animal models.Several studies have indicated that cell cycle re-enter plays a major role in neuronal apoptosis.Currently,a study was undertaken to investigate cell cycle re-entry in neuronal apoptosis after exposing to B[a]P in SD rats.Male SD rats were administrated with B[a]P by intraperitoneal injection at different dose(blank,0,1.0,2.5,6.25 mg/kg bw) for 1,2,and 3 months.Neuronal apoptotic rate of hippocampus was significantly increased in a dose and time dependent manner.To find the relationship between neuronal apoptosis and cell cycle re-entry,the cyclinDl,CDK4,Rb,pRb-ser 807/811,pRb-ser 780 and E2F1 were measured,which are related with cell cycle re-entry.Rb,pRb-ser 807/811,pRb-ser 780 and E2F1 increased in a dose and time dependent manner in hippocampus,The present results suggest that the cell cycle re-enter relates to neuronal apoptosis following exposure to B[a]P in SD rats,implying that B[a]P may be related to neurodegenerative diseases.
As a widely distributed environmental contaminant,B[a]P could cause neuronal cell apoptosis in animal models.Several studies have indicated that cell cycle re-enter plays a major role in neuronal apoptosis.Currently,a study was undertaken to investigate cell cycle re-entry in neuronal apoptosis after exposing to B[a]P in SD rats.Male SD rats were administrated with B[a]P by intraperitoneal injection at different dose(blank,0,1.0,2.5,6.25 mg/kg bw) for 1,2,and 3 months.Neuronal apoptotic rate of hippocampus was significantly increased in a dose and time dependent manner.To find the relationship between neuronal apoptosis and cell cycle re-entry,the cyclinDl,CDK4,Rb,pRb-ser 807/811,pRb-ser 780 and E2F1 were measured,which are related with cell cycle re-entry.Rb,pRb-ser 807/811,pRb-ser 780 and E2F1 increased in a dose and time dependent manner in hippocampus,The present results suggest that the cell cycle re-enter relates to neuronal apoptosis following exposure to B[a]P in SD rats,implying that B[a]P may be related to neurodegenerative diseases.
As a widely distributed environmental contaminant,B[a]P could cause neuronal cell apoptosis in animal models.Several studies have indicated that cell cycle re-enter plays a major role in neuronal apoptosis.Currently,a study was undertaken to investigate cell cycle re-entry in neuronal apoptosis after exposing to B[a]P in SD rats.Male SD rats were administrated with B[a]P by intraperitoneal injection at different dose(blank,0,1.0,2.5,6.25 mg/kg bw) for 1,2,and 3 months.Neuronal apoptotic rate of hippocampus was significantly increased in a dose and time dependent manner.To find the relationship between neuronal apoptosis and cell cycle re-entry,the cyclinDl,CDK4,Rb,pRb-ser 807/811,pRb-ser 780 and E2F1 were measured,which are related with cell cycle re-entry.Rb,pRb-ser 807/811,pRb-ser 780 and E2F1 increased in a dose and time dependent manner in hippocampus,The present results suggest that the cell cycle re-enter relates to neuronal apoptosis following exposure to B[a]P in SD rats,implying that B[a]P may be related to neurodegenerative diseases.
引文
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2.Lodovici,M.,et al.,Sidestream tobacco smoke as the main predictor of exposure to polycyclic aromatic hydrocarbons.J Appl Toxicol,2004.24(4):p.277-81.
3.Perera,F.,Carcinogenicity of airborne fine particulate benzo(a)pyrene:an appraisal of the evidence and the need for control.Environ Health Perspect,1981.42:p.163-85.
4.Phillips,D.H.,Pofycyclic aromatic hydrocarbons in the diet.Mutat Res,1999.443(1-2):p.139-47.
5.Roseiro,L.C.,et al.,Comparative survey of PAHs incidence in Portuguese traditional meat and blood sausages.Food Chem Toxicol,2012.50(6):p.1891-6.
6.Armstrong,B.G.and G.Gibbs,Exposure-response relationship between lung cancer and pofycyclic aromatic hydrocarbons(PAHs).Occup Environ Med,2009.66(11):p.740-6.
7.Cai,Y,et al.,Dietary exposure estimates of 16 pofycyclic aromatic hydrocarbons(PAHs)in Xuanwei and Fuyuan,counties in a high lung cancer incidence area in China.J Environ Monit,2012.14(3):p.886-92.
8.Tokiwa,H.,et al.,The presence of mutagens carcinogens in the excised lung and analysis of lung cancer induction.Carcinogenesis,1993.14(9):p.1933-8.
9.Kriek,E.,et al.,Formation of DNA modifications by chemical carcinogens.Identification,localization and quantification.Biochim Biophys Acta,1984.738(4):p.181-201.
10.Grova,N.,et al.,Modulation of behavior and NMDA-RI gene mRNA expression in adult female mice after sub-acute administration of benzo(a)pyrene.Neurotoxicology,2007.28(3):p.630-6.
11.Saunders,C.R.,et al.,Benzo(a)pyrene-induced acute neurotoxicity in the F-344 rat:role of oxidative stress.J Appl Toxicol,2006.26(5):p.427-38.
12.Xia,Y,et al.,Effects of subchronic exposure to benzo[a]pyrene(B[a]P)on learning and memory,and neurotransmitters in male Sprague-Dawley rat.Neurotoxicology,2011.32(2):p.188-98.
13.Kilbum,K.H.and R.H.Warshaw,Neurotoxic effects from residential exposure to chemicals from an oil reprocessing facility and superfund site.Neurotoxicol Teratol,1995.17(2):p.89-102.
14 Majchrzak,R.,J,Sroczynski,and E.Chelmecka,[Evaluation of the nervous system in workers in the furnace and coal divisions of the coke-producing plants].Med Pr,1990.41(2):p.108-13.
15.Sun,X.Q.,et al.,Simulated weightlessness aggravates hypergravity-induced impairment of learning and memory and neuronal apoptosis in rats.Behav Brain Res,2009.199(2):p.197-202.
16.Li,W.Z.,et al.,Glucocorticoids increase impairments in learning and memory due to elevated amyloid precursor protein expression and neuronal apoptosis in 12-month old mice.Eur J Pharmacol,2010.628(1-3):p.108-15.
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19.Cotman,C.W.and A.J.Anderson,A potential role for apoptosis in neurodegeneration and Alzheimer's disease.Mol Neurobiol,1995.10(1):p.19-45.
20.Nie,J.S.,et al.,Involvement of mitochondrial pathway in benzo[a]pyrene-induced neuron apoptosis.Hum Exp Toxicol,2013.
21.Nie,J.,et al.,Tau Hyperphosphorylation is Associated with Spatial Learning and Memory After Exposure to Benzo[a]pyrene in SD Rats.Neuvotox Res,2013.
22.Wen,Y.,et al.,Cell-cycle regulators are involved in transient cerebral ischemia induced neuronal apoptosis in female rats.FEBS Lett,2005.579(21):p.4591-9.
23.Vermeulen,K.,Z.N.Bememan,and D.R.Van Bockstaele,Cell cycle and apoptosis.Cell Prolif,2003.36(3):p.165-75.
24.Nguyen,M.D.,W.E.Mushynski,and J.P.Julien,Cycling at the interface between neurodevelopmeni and neurodegeneration.Cell Death Differ,2002.9(12):p.1294-306.
25.Dimova,D.K.and N.J.Dyson,The E2F transcriptional network:old acquaintances with new faces.Oncogene,2005.24(17):p.2810-26.
26.Balciunaite,E.,et al.,Pocket protein complexes are recruited to distinct targets in quiescent and proliferating cells.Mol Cell Biol,2005.25(18):p.8166-78.
27.Viatour,P.and J.Sage,Newly identified aspects of tumor suppression by RB.Dis Model Mech,2011.4(5):p.581-5.
28.Khan,M.Z.,et al.,The chemokine CXCL12 promotes survival of postmitotic neurons by regulating Rb protein.Cell Death Differ,2008.15(10):p.1663-72.
29.Yu,Y.,et al.,Phospho-Rb mediating cell cycle reentry induces early apoplosis following oxygen-glucose deprivation in rat cortical neurons.Neurochem Res,2012.37(3):p.503-11.
30.Dasgupta,P.,J.Padmanabhan,and S.Chellappan,Rb function in the apoplosis and senescence of non-neuronal and neuronal cells:role in oncogenesis.Curr Mol Med,2006.6(7):p.719-29.
31.Yu,Y,et al..The involvement of upregulalion and translocation of phospho-Rb in early neuronal apoplosis following focal cerebral ischemia in rats.Neurochem Res,2009.34(6):p.1113-9.
32.Horn,H.F.and K.H.Vousden,Coping with stress:multiple ways to activate p53.Oncogene,2007.26(9):p.1306-16.
33.Boggs,K.and D.Reisman,C/EBPbetaparticipates in regulating transcription of the p53 gene in response to mitogen stimulation.J Biol Chem,2007.282(11):p.7982-90.
34.Boggs,K.,B.Henderson,and D.Reisman,RBP-Jkappa binds to and represses transcription of the p53 tumor suppressor gene.Cell Biol Int,2009.33(3):p.318-24.
35.Sebastian,T.,et al.,CEBPbeta cooperates with RB:E2F to implement Ras(VI2)-induced cellular senescence.EMBO J,2005.24(18):p.3301-12.
36.Yu,J.and L.Zhang,The transcriptional targets of p53 in apoplosis control.Biochem Biopbys Res Common,2005.331(3):p.851-8.
37.Wolter,K.G.,et al.,Movement of Bax from the cytosol to mitochondria during apoptosis.J Cell Biol,1997.139(5):p.1281-92.
38.Youle,R.J.and A.Strasser,The BCL-2 protein family:opposing activities that mediate cell death.Nat Rev Mol Cell Biol,2008.9(1):p.47-59.
39.Wang,Q,et al.,Mitochondrial signaling pathway is also involved in bisphenol A induced germ cell apoplosis in testes.Toxicol Lett,2010.199(2):p.129-35.
40.Lejeune,M.,et al.,Quantification of diverse subcellular immunohistochemical markers with clinicobiological relevancies:validation of a new computer-assisted image analysis procedure.J Anat,2008.212(6):p.868-78.
41.Languren,G.,et al.,Neuronal damage and cognitive impairment associated with hypoglycemia:An integrated view.Neurochem Int,2013.63(4):p.331-43.
42.Fito,M.,R.de la Torre,and M.I.Covas,Olive oil and oxidative stress.Mol Nutr Food Res,2007.51(10):p.1215-24.
43.Zamani,M.,et al.,Neuroprotective effect of olive oil in the hippocampus CA1 neurons following ischemia:Reperfusion in mice.J Neurosci Rural Pract,2013.4(2):p.164-70.
44.Persson,E.,P.Larsson,and H.Tjalve,Cellular activation and neuronal transport of mtranasally instilled benzo(a)pyrene in the olfactory system of rats.Toxicol Lett,2002.133(2-3):p.211-9.
45.Das,M.,P.K.Seth,and H.Mukhtar,Distribution of benzo(a)pyrene in discrete regions of rat brain.Bull Environ Contam Toxicol,1985.35(4):p.500-4.
46.Qiu,C,et al.,Effects ofsubchronic benzo(a)pyrene exposure on neurotransmitter receptor gene expression in the rat hippocampus related with spatial learning and memory change.Toxicology,2011.289(2-3):p.83-90.
47.Hattemer-Frey,H.A.and C.C.Travis,Benzo-a-pyrene:environmental partitioning and human exposure.Toxicol Ind Health,1991.7(3):p.141-57.
48.Falco,G.,et al.,Polycyclic aromatic hydrocarbons in foods:human exposure through the diet in Catalonia,Spain.J Food Prot,2003.66(12):p.2325-31.
49.de Vos,R.H.,et al.,Polycyclic aromatic hydrocarbons in Dutch total diet samples(1984-1986).Food Chem Toxicol,1990.28(4):p.263-8.
50.Nwaneshiudu,O.C.,et al.,Risk of polycyclic aromatic hydrocarbon(PAH)exposure from ingested food:the Azerbaijan case study.J Environ Sci Health B,2007.42(2):p.201-9.
51.Grimmer,G.,K.W.Naujack,and G,Dettbam,Gaschromatographic determination of polycyclic aromatic hydrocarbons,aza-arenes,aromatic amines in the particle and vapor phase of mainstream and sidestream smoke of cigarettes.Toxicol Lett,1987.35(1):p.117-24.
52.Pan,C.H.,et al.,Reduction of cooking oil fume exposure following an engineering intervention in Chinese restaurants.Occup Environ Med,2011.68(1):p.10-5.
53.Paul,CM.,G.Magda,and S.Abel,Spatial memory:Tlieoretical basis and comparative review on experimental methods in rodents.Behav Brain Res,2009.203(2):p.151-64.
54.Scott,G.A.,M.Mtetwa,and H.Lehmann,Novel odour recognition memory is independent of the hippocampus in rats.Exp Brain Res,2013.224(2):p.199-209.
55.O'Keefe,J.and J.Dostrovsky,The hippocampus as a spatial map.Preliminary evidence from unit activity in the freely-moving rat.Brain Res,1971.34(1):p.171-5.
56.Winocur,G.,et al.,Retrograde amnesia in rats with lesions to the hippocampus on a test of spatial memory.Neuropsychologia,2005.43(11):p.1580-1590.
57.Cipolotti,L.,et al.,Long-term retrograde amnesia...the crucial role of the hippocampus.Neuropsychologia,2001.39(2):p.151-172.
58.Zhao,J.,et al.,[Effects of benzo(a)pyrene on apoptosis of neuronal cells and expression of Bcl-2 and Bax proteins in rat brain tissue].Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi,2011.29(11).p.820-4.
59.Lossi,L.and A.Merighi,In vivo cellular and molecular mechanisms of neuronal apoptosis in the mammalian CNS.Prog Neurobiol,2003.69(5):p.287-312.
60.Beijersbergen,R.L.,et al.,Regulation of the retinoblastoma protein-related p107 by G1 cyclin complexes.Genes Dev,1995.9(11):p.1340-53.
61.Osuga,H.,et al.,Cyclin-dependent kinases as a therapeutic target for stroke.Proc Natl Acad Sci U S A,2000.97(18):p.10254-9.
62.Reisman,D.,et al.,Transcriptional Regulation of the p53 Tumor Suppressor Gene in S-Phase of the Cell-Cycle and the Cellular Response to DNA Damage.Biochem Res Int,2012.2012:p.808934.
63.Takahashi,P.,A.Poison,and D.Reisman,Elevated transcription of the p53 gene in early S-phase leads to a rapid DNA-damage response during S-phase of the cell cycle.Apoptosis,2011.16(9):p.950-8.
64.Futatsugi,A.,et al.,Cyclm-dependent kinase S regulates E2F transcription factor through phosphorylation of Rb protein in neurons.Cell Cycle,2012.11(8):p.1603-10.
2.Lodovici,M.,et al.,Sidestream tobacco smoke as the main predictor of exposure to polycyclic aromatic hydrocarbons.J Appl Toxicol,2004.24(4):p.277-81.
3.Perera,F.,Carcinogenicity of airborne fine particulate benzo(a)pyrene:an appraisal of the evidence and the need for control.Environ Health Perspect,1981.42:p.163-85.
4.Phillips,D.H.,Pofycyclic aromatic hydrocarbons in the diet.Mutat Res,1999.443(1-2):p.139-47.
5.Roseiro,L.C.,et al.,Comparative survey of PAHs incidence in Portuguese traditional meat and blood sausages.Food Chem Toxicol,2012.50(6):p.1891-6.
6.Armstrong,B.G.and G.Gibbs,Exposure-response relationship between lung cancer and pofycyclic aromatic hydrocarbons(PAHs).Occup Environ Med,2009.66(11):p.740-6.
7.Cai,Y,et al.,Dietary exposure estimates of 16 pofycyclic aromatic hydrocarbons(PAHs)in Xuanwei and Fuyuan,counties in a high lung cancer incidence area in China.J Environ Monit,2012.14(3):p.886-92.
8.Tokiwa,H.,et al.,The presence of mutagens carcinogens in the excised lung and analysis of lung cancer induction.Carcinogenesis,1993.14(9):p.1933-8.
9.Kriek,E.,et al.,Formation of DNA modifications by chemical carcinogens.Identification,localization and quantification.Biochim Biophys Acta,1984.738(4):p.181-201.
10.Grova,N.,et al.,Modulation of behavior and NMDA-RI gene mRNA expression in adult female mice after sub-acute administration of benzo(a)pyrene.Neurotoxicology,2007.28(3):p.630-6.
11.Saunders,C.R.,et al.,Benzo(a)pyrene-induced acute neurotoxicity in the F-344 rat:role of oxidative stress.J Appl Toxicol,2006.26(5):p.427-38.
12.Xia,Y,et al.,Effects of subchronic exposure to benzo[a]pyrene(B[a]P)on learning and memory,and neurotransmitters in male Sprague-Dawley rat.Neurotoxicology,2011.32(2):p.188-98.
13.Kilbum,K.H.and R.H.Warshaw,Neurotoxic effects from residential exposure to chemicals from an oil reprocessing facility and superfund site.Neurotoxicol Teratol,1995.17(2):p.89-102.
14 Majchrzak,R.,J,Sroczynski,and E.Chelmecka,[Evaluation of the nervous system in workers in the furnace and coal divisions of the coke-producing plants].Med Pr,1990.41(2):p.108-13.
15.Sun,X.Q.,et al.,Simulated weightlessness aggravates hypergravity-induced impairment of learning and memory and neuronal apoptosis in rats.Behav Brain Res,2009.199(2):p.197-202.
16.Li,W.Z.,et al.,Glucocorticoids increase impairments in learning and memory due to elevated amyloid precursor protein expression and neuronal apoptosis in 12-month old mice.Eur J Pharmacol,2010.628(1-3):p.108-15.
17.LaFerla,F.M.,et al.,The Alzheimer's A beta peptide induces neurodegeneration and apoptotic cell death in transgenic mice.Nat Genet,1995.9(1):p.21-30.
18.Roth,K.A.and J.J.Shacka,Apoptosis in Neurodegenerative Disease,in Encyclopedia of Neuroscience,R.S.Editor-in-Chief:Larry,Editor.2009,Academic Press:Oxford,p.531-537.
19.Cotman,C.W.and A.J.Anderson,A potential role for apoptosis in neurodegeneration and Alzheimer's disease.Mol Neurobiol,1995.10(1):p.19-45.
20.Nie,J.S.,et al.,Involvement of mitochondrial pathway in benzo[a]pyrene-induced neuron apoptosis.Hum Exp Toxicol,2013.
21.Nie,J.,et al.,Tau Hyperphosphorylation is Associated with Spatial Learning and Memory After Exposure to Benzo[a]pyrene in SD Rats.Neuvotox Res,2013.
22.Wen,Y.,et al.,Cell-cycle regulators are involved in transient cerebral ischemia induced neuronal apoptosis in female rats.FEBS Lett,2005.579(21):p.4591-9.
23.Vermeulen,K.,Z.N.Bememan,and D.R.Van Bockstaele,Cell cycle and apoptosis.Cell Prolif,2003.36(3):p.165-75.
24.Nguyen,M.D.,W.E.Mushynski,and J.P.Julien,Cycling at the interface between neurodevelopmeni and neurodegeneration.Cell Death Differ,2002.9(12):p.1294-306.
25.Dimova,D.K.and N.J.Dyson,The E2F transcriptional network:old acquaintances with new faces.Oncogene,2005.24(17):p.2810-26.
26.Balciunaite,E.,et al.,Pocket protein complexes are recruited to distinct targets in quiescent and proliferating cells.Mol Cell Biol,2005.25(18):p.8166-78.
27.Viatour,P.and J.Sage,Newly identified aspects of tumor suppression by RB.Dis Model Mech,2011.4(5):p.581-5.
28.Khan,M.Z.,et al.,The chemokine CXCL12 promotes survival of postmitotic neurons by regulating Rb protein.Cell Death Differ,2008.15(10):p.1663-72.
29.Yu,Y.,et al.,Phospho-Rb mediating cell cycle reentry induces early apoplosis following oxygen-glucose deprivation in rat cortical neurons.Neurochem Res,2012.37(3):p.503-11.
30.Dasgupta,P.,J.Padmanabhan,and S.Chellappan,Rb function in the apoplosis and senescence of non-neuronal and neuronal cells:role in oncogenesis.Curr Mol Med,2006.6(7):p.719-29.
31.Yu,Y,et al..The involvement of upregulalion and translocation of phospho-Rb in early neuronal apoplosis following focal cerebral ischemia in rats.Neurochem Res,2009.34(6):p.1113-9.
32.Horn,H.F.and K.H.Vousden,Coping with stress:multiple ways to activate p53.Oncogene,2007.26(9):p.1306-16.
33.Boggs,K.and D.Reisman,C/EBPbetaparticipates in regulating transcription of the p53 gene in response to mitogen stimulation.J Biol Chem,2007.282(11):p.7982-90.
34.Boggs,K.,B.Henderson,and D.Reisman,RBP-Jkappa binds to and represses transcription of the p53 tumor suppressor gene.Cell Biol Int,2009.33(3):p.318-24.
35.Sebastian,T.,et al.,CEBPbeta cooperates with RB:E2F to implement Ras(VI2)-induced cellular senescence.EMBO J,2005.24(18):p.3301-12.
36.Yu,J.and L.Zhang,The transcriptional targets of p53 in apoplosis control.Biochem Biopbys Res Common,2005.331(3):p.851-8.
37.Wolter,K.G.,et al.,Movement of Bax from the cytosol to mitochondria during apoptosis.J Cell Biol,1997.139(5):p.1281-92.
38.Youle,R.J.and A.Strasser,The BCL-2 protein family:opposing activities that mediate cell death.Nat Rev Mol Cell Biol,2008.9(1):p.47-59.
39.Wang,Q,et al.,Mitochondrial signaling pathway is also involved in bisphenol A induced germ cell apoplosis in testes.Toxicol Lett,2010.199(2):p.129-35.
40.Lejeune,M.,et al.,Quantification of diverse subcellular immunohistochemical markers with clinicobiological relevancies:validation of a new computer-assisted image analysis procedure.J Anat,2008.212(6):p.868-78.
41.Languren,G.,et al.,Neuronal damage and cognitive impairment associated with hypoglycemia:An integrated view.Neurochem Int,2013.63(4):p.331-43.
42.Fito,M.,R.de la Torre,and M.I.Covas,Olive oil and oxidative stress.Mol Nutr Food Res,2007.51(10):p.1215-24.
43.Zamani,M.,et al.,Neuroprotective effect of olive oil in the hippocampus CA1 neurons following ischemia:Reperfusion in mice.J Neurosci Rural Pract,2013.4(2):p.164-70.
44.Persson,E.,P.Larsson,and H.Tjalve,Cellular activation and neuronal transport of mtranasally instilled benzo(a)pyrene in the olfactory system of rats.Toxicol Lett,2002.133(2-3):p.211-9.
45.Das,M.,P.K.Seth,and H.Mukhtar,Distribution of benzo(a)pyrene in discrete regions of rat brain.Bull Environ Contam Toxicol,1985.35(4):p.500-4.
46.Qiu,C,et al.,Effects ofsubchronic benzo(a)pyrene exposure on neurotransmitter receptor gene expression in the rat hippocampus related with spatial learning and memory change.Toxicology,2011.289(2-3):p.83-90.
47.Hattemer-Frey,H.A.and C.C.Travis,Benzo-a-pyrene:environmental partitioning and human exposure.Toxicol Ind Health,1991.7(3):p.141-57.
48.Falco,G.,et al.,Polycyclic aromatic hydrocarbons in foods:human exposure through the diet in Catalonia,Spain.J Food Prot,2003.66(12):p.2325-31.
49.de Vos,R.H.,et al.,Polycyclic aromatic hydrocarbons in Dutch total diet samples(1984-1986).Food Chem Toxicol,1990.28(4):p.263-8.
50.Nwaneshiudu,O.C.,et al.,Risk of polycyclic aromatic hydrocarbon(PAH)exposure from ingested food:the Azerbaijan case study.J Environ Sci Health B,2007.42(2):p.201-9.
51.Grimmer,G.,K.W.Naujack,and G,Dettbam,Gaschromatographic determination of polycyclic aromatic hydrocarbons,aza-arenes,aromatic amines in the particle and vapor phase of mainstream and sidestream smoke of cigarettes.Toxicol Lett,1987.35(1):p.117-24.
52.Pan,C.H.,et al.,Reduction of cooking oil fume exposure following an engineering intervention in Chinese restaurants.Occup Environ Med,2011.68(1):p.10-5.
53.Paul,CM.,G.Magda,and S.Abel,Spatial memory:Tlieoretical basis and comparative review on experimental methods in rodents.Behav Brain Res,2009.203(2):p.151-64.
54.Scott,G.A.,M.Mtetwa,and H.Lehmann,Novel odour recognition memory is independent of the hippocampus in rats.Exp Brain Res,2013.224(2):p.199-209.
55.O'Keefe,J.and J.Dostrovsky,The hippocampus as a spatial map.Preliminary evidence from unit activity in the freely-moving rat.Brain Res,1971.34(1):p.171-5.
56.Winocur,G.,et al.,Retrograde amnesia in rats with lesions to the hippocampus on a test of spatial memory.Neuropsychologia,2005.43(11):p.1580-1590.
57.Cipolotti,L.,et al.,Long-term retrograde amnesia...the crucial role of the hippocampus.Neuropsychologia,2001.39(2):p.151-172.
58.Zhao,J.,et al.,[Effects of benzo(a)pyrene on apoptosis of neuronal cells and expression of Bcl-2 and Bax proteins in rat brain tissue].Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi,2011.29(11).p.820-4.
59.Lossi,L.and A.Merighi,In vivo cellular and molecular mechanisms of neuronal apoptosis in the mammalian CNS.Prog Neurobiol,2003.69(5):p.287-312.
60.Beijersbergen,R.L.,et al.,Regulation of the retinoblastoma protein-related p107 by G1 cyclin complexes.Genes Dev,1995.9(11):p.1340-53.
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