以金纳米粒子为共振光散射技术探针灵敏检测桑色素和杨梅苷
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- 英文论文题名：Using Gold Nanoparticles as Probe for Sensitive Determination of Morin Hydrate and Myricetin by Resonance Rayleigh Light Scattering
- 论文作者：毕淑云 ; 周慧凤 ; 王瑜
- 英文论文作者：Shuyun Bi ; Huifeng Zhou ; Yu Wang ; College of Chemistry ; Changchun Normal University
- 年：2016
- 作者机构：长春师范大学化学学院;
- 论文关键词：共振光散射技术(RLS) ; 金纳米粒子(AuNPs) ; 桑色素 ; 杨梅苷
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十八分会：纳米生物效应与纳米药物化学
- 语种：中文
- 分类号：O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十八分会 ; 纳米生物效应与纳米药物化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：266k
- 原文格式：D
- 会议级别：全国
摘要
利用种子生长法合成的金纳米粒子(AuN Ps)为共振光散射技术(RLS)探针灵敏检测两种黄酮类药物—桑色素(morin hydrate)和杨梅苷(myricetin)。通过静电引力,AuN Ps分别与桑色素和杨梅苷结合,形成二元复合物,使RLS强度增强(Fig.1)。在最佳实验条件下,体系的RLS强度增加值ΔIRLS与Au NPs的浓度呈良好的线性关系,线性范围分别为0.1208~3.02μg mL-1和0.0464~4.64μg mL~(-1),检出限分别为2.98 ng mL~(-1)和1.39ng mL~(-1)。应用该方法分别检测了合成样品中的桑色素和杨梅苷,相对标准偏差(RSD)分别为0.79—2.1%和0.7—2.5%(n=5),回收率分别为99.7—101.6%和97.2—103.4%。
The morin hydrate and myricetin were determined using gold nanoparticles(Au NPs) as probes respectively by Resonance Rayleigh Light Scattering(RLS). The morin hydrate and myricetin were bond to AuN Ps by electrostatic interaction, which leads to an obviously enhancement of the RLS intensity. It was found that the enhanced RLS intensity was proportional to the concentration of morin hydrate and myricetin in the range of 0.1208—3.02 μg mL~(-1) and 0.0464—4.64 μg mL~(-1) with the detection limits of 2.98 ng mL~(-1) and 1.39 ng mL~(-1), respectively. The recoveries were 99.7—101.6% and 97.2—103.4% and RSD were 0.79—2.1% and 0.7—2.5%, respectively.
The morin hydrate and myricetin were determined using gold nanoparticles(Au NPs) as probes respectively by Resonance Rayleigh Light Scattering(RLS). The morin hydrate and myricetin were bond to AuN Ps by electrostatic interaction, which leads to an obviously enhancement of the RLS intensity. It was found that the enhanced RLS intensity was proportional to the concentration of morin hydrate and myricetin in the range of 0.1208—3.02 μg mL~(-1) and 0.0464—4.64 μg mL~(-1) with the detection limits of 2.98 ng mL~(-1) and 1.39 ng mL~(-1), respectively. The recoveries were 99.7—101.6% and 97.2—103.4% and RSD were 0.79—2.1% and 0.7—2.5%, respectively.
引文
[1]陶俊.金属纳米颗粒及其聚合物复合薄膜的光学特性研究[博士学位论文],2011,pp 27.
[2]Stobiecka M.;Deeb J.;Hepel M.Biophys.Chem.2010,146:98.
[2]Stobiecka M.;Deeb J.;Hepel M.Biophys.Chem.2010,146:98.