摘要
利用种子生长法合成的金纳米粒子(AuN Ps)为共振光散射技术(RLS)探针灵敏检测两种黄酮类药物—桑色素(morin hydrate)和杨梅苷(myricetin)。通过静电引力,AuN Ps分别与桑色素和杨梅苷结合,形成二元复合物,使RLS强度增强(Fig.1)。在最佳实验条件下,体系的RLS强度增加值ΔIRLS与Au NPs的浓度呈良好的线性关系,线性范围分别为0.1208~3.02μg mL-1和0.0464~4.64μg mL~(-1),检出限分别为2.98 ng mL~(-1)和1.39ng mL~(-1)。应用该方法分别检测了合成样品中的桑色素和杨梅苷,相对标准偏差(RSD)分别为0.79—2.1%和0.7—2.5%(n=5),回收率分别为99.7—101.6%和97.2—103.4%。
The morin hydrate and myricetin were determined using gold nanoparticles(Au NPs) as probes respectively by Resonance Rayleigh Light Scattering(RLS). The morin hydrate and myricetin were bond to AuN Ps by electrostatic interaction, which leads to an obviously enhancement of the RLS intensity. It was found that the enhanced RLS intensity was proportional to the concentration of morin hydrate and myricetin in the range of 0.1208—3.02 μg mL~(-1) and 0.0464—4.64 μg mL~(-1) with the detection limits of 2.98 ng mL~(-1) and 1.39 ng mL~(-1), respectively. The recoveries were 99.7—101.6% and 97.2—103.4% and RSD were 0.79—2.1% and 0.7—2.5%, respectively.
引文
[1]陶俊.金属纳米颗粒及其聚合物复合薄膜的光学特性研究[博士学位论文],2011,pp 27.
[2]Stobiecka M.;Deeb J.;Hepel M.Biophys.Chem.2010,146:98.