Paraquat and MPTP induce alterations in the expression profiles of long noncoding RNAs in the substantia nigra of mice:Role of the transcription factor Nrf2
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- 英文论文题名：Paraquat and MPTP induce alterations in the expression profiles of long noncoding RNAs in the substantia nigra of mice:Role of the transcription factor Nrf2
- 论文作者：Lijin Wang ; Hongyu Yang ; Qingqing Wang ; Qiaohui Zhang ; Zhangjing Wang ; Qunwei Zhang ; Siying Wu ; Huangyuan Li
- 英文论文作者：Lijin Wang ; Hongyu Yang ; Qingqing Wang ; Qiaohui Zhang ; Zhangjing Wang ; Qunwei Zhang ; Siying Wu ; Huangyuan Li ; Department of Preventive Medicine ; Fujian Provincial Key Laboratory of Environment factors and Cancer ; School of Public Health ; Fujian Medical University ; Department of Endemic Disease Prevention and Control ; Fujian Center For Disease Control & Prevention ; Department of Epidemiology and Health Statistics ; Fujian Provincial Key Laboratory of Environment factors and Cancer ; School of Public Health ; Fujian Medical University
- 年：2016
- 作者机构：Department of Preventive Medicine,Fujian Provincial Key Laboratory of Environment factors and Cancer,School of Public Health,Fujian Medical University;Department of Endemic Disease Prevention and Control,Fujian Center For Disease Control & Prevention;Department of Epidemiology and Health Statistics,Fujian Provincial Key Laboratory of Environment factors and Cancer,School of Public Health,Fujian Medical University;
- 英文论文关键词：Paraquat ; 1-Methyl-4-phenyl-1 ; 2 ; 3 ; 6-tetrahydropyridine ; Neurodegeneration ; LncRNA ; Nrf2 ; Epigenetic toxicology
- 会议召开时间：2016-12-03
- 会议录名称：2016中国毒理学会神经毒理专业委员会学术年会论文集
- 语种：英文
- 分类号：R742.5
- 学会代码：ZGDV
- 会议名称：2016中国毒理学会神经毒理专业委员会学术年会
- 会议地点：中国北京
- 主办单位：中国毒理学会神经毒理专业委员会
- 学会名称：中国毒理学会
- 页数：1
- 文件大小：61k
- 原文格式：O
- 会议级别：全国
摘要
Parkinson's disease(PD) is a common age-related degenerative disease of the central nervous system caused by hereditary,pesticides,metals,and polychlorinated biphenyls.Paraquat(PQ),a widely used herbicide,causes PD.Long noncoding RNAs(lncRNAs) are nonprotein-coding transcripts,expressed in the brain and play irreplaceable roles in neurodegenerative diseases.Nrf2 is an important genetic transcription regulator in oxidative stress.We aimed to discover novel PQ or MPTP-Nrf2-related lncRNAs and explore their associations with PD.First,a IncRNA array was used to identify 17157 lncRNAs and 13707 mRNAs on a Mouse LncRNA Expression Microarray(fold change ≥2,p-value<0.05).Then,several lncRNAs were double-checked using real-time quantitative reverse transcription PCR(qRT-PCR) and fluorescence in situ hybridization.Coding-noncoding analysis and qRT-PCR were applied to discover the functions of lncRNAs and predict the targeted genes.In vivo,PQ and1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) exposure changed the IncRNA expression profiles,probably related to their neurotoxic mechanism.The changes in their IncRNA expression were distinct but related.PQ changed the IncRNA expression profiling in the substantia nigra(SN) through an interaction with Nrf2,thus changing the NR_027648/Zc3hl4/Cybb and NR_030777/Zfp326/Cpne5 mRNA pathways.Similarly,MPTP changed the IncRNA expression profiling in SN through an interaction with Nrf2.Nrf2 may be involved in the development of neurodegeneration induced by PQ and MPTP via interactions of lncRNAs as the molecular mechanism.Our findings,for the first time,indicate the potential roles of lncRNAs in the development of PD by PQ or MPTP and provide positive insights into future mechanism studies.
Parkinson's disease(PD) is a common age-related degenerative disease of the central nervous system caused by hereditary,pesticides,metals,and polychlorinated biphenyls.Paraquat(PQ),a widely used herbicide,causes PD.Long noncoding RNAs(lncRNAs) are nonprotein-coding transcripts,expressed in the brain and play irreplaceable roles in neurodegenerative diseases.Nrf2 is an important genetic transcription regulator in oxidative stress.We aimed to discover novel PQ or MPTP-Nrf2-related lncRNAs and explore their associations with PD.First,a IncRNA array was used to identify 17157 lncRNAs and 13707 mRNAs on a Mouse LncRNA Expression Microarray(fold change ≥2,p-value<0.05).Then,several lncRNAs were double-checked using real-time quantitative reverse transcription PCR(qRT-PCR) and fluorescence in situ hybridization.Coding-noncoding analysis and qRT-PCR were applied to discover the functions of lncRNAs and predict the targeted genes.In vivo,PQ and1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) exposure changed the IncRNA expression profiles,probably related to their neurotoxic mechanism.The changes in their IncRNA expression were distinct but related.PQ changed the IncRNA expression profiling in the substantia nigra(SN) through an interaction with Nrf2,thus changing the NR_027648/Zc3hl4/Cybb and NR_030777/Zfp326/Cpne5 mRNA pathways.Similarly,MPTP changed the IncRNA expression profiling in SN through an interaction with Nrf2.Nrf2 may be involved in the development of neurodegeneration induced by PQ and MPTP via interactions of lncRNAs as the molecular mechanism.Our findings,for the first time,indicate the potential roles of lncRNAs in the development of PD by PQ or MPTP and provide positive insights into future mechanism studies.
Parkinson's disease(PD) is a common age-related degenerative disease of the central nervous system caused by hereditary,pesticides,metals,and polychlorinated biphenyls.Paraquat(PQ),a widely used herbicide,causes PD.Long noncoding RNAs(lncRNAs) are nonprotein-coding transcripts,expressed in the brain and play irreplaceable roles in neurodegenerative diseases.Nrf2 is an important genetic transcription regulator in oxidative stress.We aimed to discover novel PQ or MPTP-Nrf2-related lncRNAs and explore their associations with PD.First,a IncRNA array was used to identify 17157 lncRNAs and 13707 mRNAs on a Mouse LncRNA Expression Microarray(fold change ≥2,p-value<0.05).Then,several lncRNAs were double-checked using real-time quantitative reverse transcription PCR(qRT-PCR) and fluorescence in situ hybridization.Coding-noncoding analysis and qRT-PCR were applied to discover the functions of lncRNAs and predict the targeted genes.In vivo,PQ and1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) exposure changed the IncRNA expression profiles,probably related to their neurotoxic mechanism.The changes in their IncRNA expression were distinct but related.PQ changed the IncRNA expression profiling in the substantia nigra(SN) through an interaction with Nrf2,thus changing the NR_027648/Zc3hl4/Cybb and NR_030777/Zfp326/Cpne5 mRNA pathways.Similarly,MPTP changed the IncRNA expression profiling in SN through an interaction with Nrf2.Nrf2 may be involved in the development of neurodegeneration induced by PQ and MPTP via interactions of lncRNAs as the molecular mechanism.Our findings,for the first time,indicate the potential roles of lncRNAs in the development of PD by PQ or MPTP and provide positive insights into future mechanism studies.
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