黑曲霉发酵粗酶液对乌龙茶粉香味的影响
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摘要
本文利用茶梗和麦麸两种不同原料发酵的黑曲霉发酵的粗酶液处理茶粉样品并进行GC-MS分析以及感官评价。结果显示经过两种粗酶液处理后,感官评价在青草味、水果味、花香味、发酵味、甜味以及焦糖味上均具有显著性差异。其中,粗酶液处理后样品中青草味的显著提升可能与呈现青草味的3-己烯-醇和2-戊基-呋喃含量的显著增加有关。与此同时,甜味的显著提升可能与呈现甜味的苯甲醛的含量的显著性提升有关。本文发现茶梗发酵产生的粗酶液可以有效地提高乌龙茶粉的青草香味,而麦麸发酵产生的粗酶液可以有效地提高茶粉中的甜味。该研究结果表明酶处理可以有效地改变茶粉的香气,为之后酶处理改善茶粉香气的研究提供了理论依据。
Two different enzymes fermented by Aspergillus niger,which use wheat bran and tea stalk as medium,respectively,were prepared to treated the instant oolong tea,and then analyzed by GC-MS and sensory evaluation.Sensory evaluation showed significantly difference among grass,fruit,fermented,sweet,floral and roasty between instant tea and enzymatic treated samples.Among these volatiles,20 compounds have significantly increased in content,especially,cis-3-hexen-l-ol and 2-pentylfuran,which presented green and grass notes may related to the increase of grass note.In the same time,benzaldehyde,which show honey-like note and has low threshold-value,has greatly increased which may related to the increase of sweet note.This study demonstrated that the tea stalk enzymatic treated samples have significantly improved the grass note and the wheat bran enzymatic treated samples have greatly improved in the sweet odor.The result indicated that the crude enzyme can change the aroma of instant oolong tea,which may provide the theoretical basis for the research of aroma of instant tea in the future.
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