斜带石斑鱼BCL2家族蛋白BOK基因鉴定与功能分析
详细信息 查看官网全文
- 英文论文题名：Identification of the Bcl-2 family protein gene BOK from orange-spotted grouper(Epinephelus coioides) involved in SGIV infection
- 论文作者：蔡佳 ; 喻大鹏 ; 魏世娜 ; 汤菊芬 ; 鲁义善 ; 吴灶和 ; 秦启伟 ; 简纪常
- 英文论文作者：CAIJia ; YU Dapeng ; WEIShina ; TANGJufen ; LUYishan ; WUZaohe ; QIN Qiwei ; JIANJichang ; College of Fishery ; Guangdong Ocean University ; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals ; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals ; Key Laboratory of Tropical Marine Bio-resources and Ecology ; South China Sea Institute of Oceanology ; Chinese Academy of Sciences
- 年：2016
- 作者机构：广东海洋大学;广东省水产经济动物病原生物学及流行病学重点实验室;广东省教育厅水产经济动物病害控制重点实验室;中国科学院南海海洋研究所,中国科学院热带海洋生物资源与生态重点实验室;
- 论文关键词：斜带石斑鱼 ; BOK ; 新加坡虹彩病毒(SGIV) ; 病毒基因 ; 病毒复制
- 英文论文关键词：Epinephelus coioides ; BOK ; Singapore grouper iridovirus(SGIV) ; Viral gene ; Viral replication
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S943
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：563k
- 原文格式：D
- 会议级别：全国
摘要
细胞凋亡在许多生理过程和免疫应答中发挥着重要的作用,BOK基因是细胞凋亡中一个关键的调控因子。本研究对斜带石斑鱼BOK(Ec-BOK)基因进行了克隆及功能分析。结果表明,Ec-BOK基因可以编码210个氨基酸,其含有4个保守的功能结构域(BH1-4)和1个跨膜区(TM),蛋白序列与深裂眶锯雀鲷该蛋白相似度最高,为97%。荧光定量分析表明Ec-BOK基因在多种组织中均有表达,其中肝脏和肾脏中表达量最高。经SGIV感染后,Ec-BOK基因表达上调,体外实验过表达Ec-BOK基因可以抑制病毒基因的转录。本研究结果表明,Ec-BOK基因在鱼类抗病毒免疫应答中可能发挥着重要作用
Apoptosis plays vital roles in many physiological process and immune response. BOK is one of the centralregulators in apoptosis. In this study, a new BOK homolog(Ec-BOK) was cloned and characterized from Orange-spotted grouper, Epinephelus coioides. Ec-BOK encoded a210 amino acid peptides which shared97% identity to Stegastes partitus BOK protein, contained four BH domains and one transmembrane region. Ec-BOK widely expressed in all analyzed tissues with the higher expressions in kidney and spleen.Its expression level was up-regulated after SGIV infection in vitro. Further analysis revealed that overexpression of Ec-BOK inhibited viral genes transcriptions and virus replication in fish cell. Our findingssuggested that Ec-BOK might play a role in the immune response against virus.
Apoptosis plays vital roles in many physiological process and immune response. BOK is one of the centralregulators in apoptosis. In this study, a new BOK homolog(Ec-BOK) was cloned and characterized from Orange-spotted grouper, Epinephelus coioides. Ec-BOK encoded a210 amino acid peptides which shared97% identity to Stegastes partitus BOK protein, contained four BH domains and one transmembrane region. Ec-BOK widely expressed in all analyzed tissues with the higher expressions in kidney and spleen.Its expression level was up-regulated after SGIV infection in vitro. Further analysis revealed that overexpression of Ec-BOK inhibited viral genes transcriptions and virus replication in fish cell. Our findingssuggested that Ec-BOK might play a role in the immune response against virus.
引文