Overexpression of the maize psbA gene enhances sulfur dioxide tolerance in transgenic tobacco
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- 英文论文题名：Overexpression of the maize psbA gene enhances sulfur dioxide tolerance in transgenic tobacco
- 论文作者：Xinhong Su ; Peng Zhou ; Ran Wang ; Zhaopeng Luo ; Zongliang Xia
- 英文论文作者：Xinhong Su ; Peng Zhou ; Ran Wang ; Zhaopeng Luo ; Zongliang Xia ; Henan Agricultural University ; Henan Provincial Tobacco Company ; Zhengzhou Tobacco Research Institute of CNTC
- 年：2016
- 作者机构：Henan Agricultural University;Henan Provincial Tobacco Company;Zhengzhou Tobacco Research Institute of CNTC;
- 英文论文关键词：Maize ; psbA ; sulfur dioxide ; antioxidant enzyme
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：英文
- 分类号：X51;S572
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：16
- 文件大小：1393k
- 原文格式：D
摘要
The gaseous pollutant SO_2 impacts deleteriously on crop growth and development. To identify genes required for SO_2 stress response in maize(Zea mays. L), one highly induced m RNA encoding a D1 protein of photosystem II(ZmpsbA) was isolated by mRNA differential display. Real-time PCR analysis indicated that the transcript levels of ZmpsbA were higher in leaves and were markedly up-regulated by SO_2 stress. Overexpression of ZmpsbA enhanced SO_2 stress tolerance in tobacco; showing much less damage upon SO_2 exposure. The transgenic tobacco lines had higher proline accumulation, but lower levels of malondialdehyde(MDA) when compared with the wild-type plants under SO_2 stress. Further investigation showed that ZmpsbA transgenic plants displayed higher SOD and CAT activities, increased SOD and CAT transcript levels, and decreased reactive oxygen species(ROS) accumulation under SO_2 stress. Taken together, our results demonstrate that ZmpsbA confers SO_2 stress tolerance in transgenic tobacco by reducing ROS accumulation and membrane damage through enhancing the antioxidant system. ZmpsbA might serve as a candidate gene in genetic improvement for environmental pollutants(SO_2 and acid rain, etc) tolerance engineering in crop plants.
The gaseous pollutant SO_2 impacts deleteriously on crop growth and development. To identify genes required for SO_2 stress response in maize(Zea mays. L), one highly induced m RNA encoding a D1 protein of photosystem II(ZmpsbA) was isolated by mRNA differential display. Real-time PCR analysis indicated that the transcript levels of ZmpsbA were higher in leaves and were markedly up-regulated by SO_2 stress. Overexpression of ZmpsbA enhanced SO_2 stress tolerance in tobacco; showing much less damage upon SO_2 exposure. The transgenic tobacco lines had higher proline accumulation, but lower levels of malondialdehyde(MDA) when compared with the wild-type plants under SO_2 stress. Further investigation showed that ZmpsbA transgenic plants displayed higher SOD and CAT activities, increased SOD and CAT transcript levels, and decreased reactive oxygen species(ROS) accumulation under SO_2 stress. Taken together, our results demonstrate that ZmpsbA confers SO_2 stress tolerance in transgenic tobacco by reducing ROS accumulation and membrane damage through enhancing the antioxidant system. ZmpsbA might serve as a candidate gene in genetic improvement for environmental pollutants(SO_2 and acid rain, etc) tolerance engineering in crop plants.
The gaseous pollutant SO_2 impacts deleteriously on crop growth and development. To identify genes required for SO_2 stress response in maize(Zea mays. L), one highly induced m RNA encoding a D1 protein of photosystem II(ZmpsbA) was isolated by mRNA differential display. Real-time PCR analysis indicated that the transcript levels of ZmpsbA were higher in leaves and were markedly up-regulated by SO_2 stress. Overexpression of ZmpsbA enhanced SO_2 stress tolerance in tobacco; showing much less damage upon SO_2 exposure. The transgenic tobacco lines had higher proline accumulation, but lower levels of malondialdehyde(MDA) when compared with the wild-type plants under SO_2 stress. Further investigation showed that ZmpsbA transgenic plants displayed higher SOD and CAT activities, increased SOD and CAT transcript levels, and decreased reactive oxygen species(ROS) accumulation under SO_2 stress. Taken together, our results demonstrate that ZmpsbA confers SO_2 stress tolerance in transgenic tobacco by reducing ROS accumulation and membrane damage through enhancing the antioxidant system. ZmpsbA might serve as a candidate gene in genetic improvement for environmental pollutants(SO_2 and acid rain, etc) tolerance engineering in crop plants.
引文
Able AJ,Guest DI,Sutherland MW(1998)Use of a new nitroblue tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoospores.Plant Physiol 117:491-499
Adir N,Zer H,Shochat S,Ohad I(2003)Photoinhibition-a historical perspective.Photosynth Res 76:343-370
Aebi H(1984)Catalase in vitro.Meth Enzymol 105:121-126
Agrawal M,Singh B,Rajput M,Marshall F,Bell JN(2003)Effect of air pollution on peri-urban agriculture:a case study.Environ Pollut 126:323-329
Apel K,Hirt H(2004)Reactive oxygen species:metabolism,oxidative stress,and signal transduction.Annu Rev Plant Biol 55:373-399
Armbruster U,Zühlke J,Rengstl B,Kreller R,Makarenko E,Rühle T,Schünemann D,Jahns P,Weisshaar B,Nickelsen J,Leister D(2010)The Arabidopsis thylakoid protein PAM68 is required for efficient D1 biogenesis and photosystem II assembly.Plant Cell 22:3439-3460
Aro EM,Mc Caffery S,Anderson JM(1994)Recovery from photoinhibition in peas(Pisum sativum L.)acclimated to varying growth irradiances.Plant Physiol 104:1033-1041
Baena-González E,Aro EM(2002)Biogenesis,assembly and turnover of photosystem II units.Philos Trans RSoc Lond B Biol Sci 357:1451-1459
Bates LS,Waldren RP,Tear ID(1973)Rapid determination of free proline for water-stress studies.Plant Soil39:205-207
Bruce N,Perez-Padilla R,Albalak R(2000)Indoor air pollution in developing countries:a major environmental and public health challenge.Bull World Health Organ 78:1078-1092
Brychkova G,Xia Z,Yang G,Yesbergenova Z,Zhang Z,Davydov O,Fluhr R,Sagi M(2007)Sulfite oxidase protects plants against sulfur dioxide toxicity.Plant J 50:696-709
Darrail NM(1989)The effect of air pollutants on physiological processes in plants.Plant Cell Environ 12:1-30
Demmig B,Bj?rkman O(1987)Comparison of the effect of excessive light on chlorophyll fluorescence(77K)and photon yield of O2 evolution in leaves of higher plants.Planta 171:171-184
Draper HH,Hadley M(1990)Malondialdehyde determination as index of lipid peroxidation.Meth Enzymol86:421-431
Ferguson IB,Watkins CB,Harman JE(1983)Inhibition by calcium of senescence of detached cucumber cotyledons.Plant Physiol 71:182-186
Giannopolitis CN,Ries SK(1977)Superoxide dismutase.I.Occurrence in higher plants.Plant Physiol 59:309-314
Jain S,Kumar D,Jain M,Chaudhary P,Deswal R,Sarin NB(2012)Ectopic overexpression of a salt stress-induced pathogenesisrelated class 10 protein(PR10)gene from peanut(Arachis hypogaea L.)affords broad spectrum abiotic stress tolerance in transgenic tobacco.Plant Cell Tiss Organ Cult 109:19-31
Klaff P,Gruissem W(1991)Changes in chloroplast m RNA stability during leaf development.Plant Cell 3:517-529
Klein RR,Mason HS,Mullet JE(1988)Light-regulated translation of chloroplast proteins.I.Transcripts of psa A-psa B,psb A,and rbc L are associated with polysomes in dark-grown and illuminated barley seedlings.J Cell Biol 106:289-301
Klein RR,Mullet JE(1987)Control of gene expression during higher plant chloroplast biogenesis.Protein synthesis and transcript levels of psb A,psa A-psa B,and rbc L in dark-grown and illuminated barley seedlings,J.Biol.Chem.262:4341-4348
Leelavathi S,Bhardwaj A,Kumar S,Dass A,Pathak R,Pandey SS,Tripathy BC,Padmalatha KV,Dhandapani G,Kanakachari M,Solanke AU,Kumar PA,Cella R,Siva Reddy V(2011)Genome-wide transcriptome and proteome analyses of tobacco psa A and psb A deletion mutants.Plant Mol Biol 76:407-423
Livaka KJ,Schmittgen TD(2001)Analysis of relative gene expression data using real-time quantitative PCRand the 2(-Delta Delta C(T))method.Methods 25:402-408
Maxwell K,Johnson GN(2000)Chlorophyll fluorescence-a practical guide.J Exp Bot 51:659-668
Meng Z,Zhang L(1992)Cytogenetic damage induced in human lymphocytes by sodium bisulfite.Mut Res298:63-69
Müller P,Li XP,Niyogi KK(2001)Non-photochemical quenching.A response to excess light energy.Plant Physiol 125:1558-1566
Nishiyama Y,Allakhverdiev SI,Murata N(2006)A new paradigm for the action of reactive oxygen species in the photoinhibition of photosystem II.Biochim Biophys Acta 1757:742-749
Rint?maki E,Kettunen R,Aro EM(1996)Differential D1 dephosphorylation in functional and photodamaged photosystem II centres.J Biol Chem 271:14870-4875
Shen Z,Ding M,Sun J,Deng S,Zhao R,Wang M,Ma X,Wang F,Zhang H,Qian Z,Hu Y,Yu R,Shen X,Chen S(2013)Overexpression of Pe HSF mediates leaf ROS homeostasis in transgenic tobacco lines grown under salt stress conditions.Plant Cell Tiss Organ Cult 115:299-308
Soitama AJ,Zhou G,Clarke AK,Oquist G,Gustafsson P and Aro EM(1996)Over-production of the D1:2protein makes synechococcus cells more tolerant to photoinhibition of photosystem II.Plant Mol Biol 30:467-478
Sun J,Li LS,Liu MQ,Wang MJ,Ding MQ,Deng SR,Lu CF,Zhou XY,Shen X,Zheng XJ,Chen SL(2010)Hydrogen peroxide and nitric oxide mediate K+/Na+homeostasis and antioxidant defense in Na Cl-stressed callus cells of two contrasting poplars.Plant Cell,Tissue Organ Cult 103:205-215
Triolo L,Binazzi A,Cagnetti P,Carconi P,Correnti A,De Luca E,Di Bonito R,Grandoni G,Mastrantonio M,Rosa S,Schimberni M,Uccelli R,Zappa G(2008)Air pollution impact assessment on agro-ecosystem and human health characterization in the area surrounding the industrial settlement of Milazzo:a multidisciplinary approach.Environ Monitor Assess 140:191-209
Xia Z,Su X,Liu J,Wang M(2013)The RING-H2 finger gene 1(RHF1)encodes an E3 ubiquitin ligase and participates in drought stress response in Nicotiana tabacum.Genetica 141:11-21
Xia Z,Sun K,Wang M,Wu K,Zhang H(2012)Overexpression of a maize sulfite oxidase gene in tobacco enhances tolerance to sulfite stress via sulfite oxidation and CAT-mediated H2O2 scavenging.PLo S One 7:e37383
Xuan N,Jin Y,Zhang H,Xie Y,Liu Y,Wang G(2011)A putative maize zinc-finger protein gene,Zm AN13,participates in abiotic stress response.Plant Cell Tiss Organ Cult 107:101-112
Yang C,Deng W,Tang N,Wang X,Yan F,Lin D,Li Z(2013)Overexpression of Zm AFB2,the maize homologue of AFB2 gene,enhances salt tolerance in transgenic tobacco.Plant Cell Tiss Organ Cult 112:171-179
Yarmolinsky D,Brychkova G,Fluhr R,Sagi M(2013)Sulfite reductase protects plants against sulfite toxicity.Plant Physiol 161:725-743
Adir N,Zer H,Shochat S,Ohad I(2003)Photoinhibition-a historical perspective.Photosynth Res 76:343-370
Aebi H(1984)Catalase in vitro.Meth Enzymol 105:121-126
Agrawal M,Singh B,Rajput M,Marshall F,Bell JN(2003)Effect of air pollution on peri-urban agriculture:a case study.Environ Pollut 126:323-329
Apel K,Hirt H(2004)Reactive oxygen species:metabolism,oxidative stress,and signal transduction.Annu Rev Plant Biol 55:373-399
Armbruster U,Zühlke J,Rengstl B,Kreller R,Makarenko E,Rühle T,Schünemann D,Jahns P,Weisshaar B,Nickelsen J,Leister D(2010)The Arabidopsis thylakoid protein PAM68 is required for efficient D1 biogenesis and photosystem II assembly.Plant Cell 22:3439-3460
Aro EM,Mc Caffery S,Anderson JM(1994)Recovery from photoinhibition in peas(Pisum sativum L.)acclimated to varying growth irradiances.Plant Physiol 104:1033-1041
Baena-González E,Aro EM(2002)Biogenesis,assembly and turnover of photosystem II units.Philos Trans RSoc Lond B Biol Sci 357:1451-1459
Bates LS,Waldren RP,Tear ID(1973)Rapid determination of free proline for water-stress studies.Plant Soil39:205-207
Bruce N,Perez-Padilla R,Albalak R(2000)Indoor air pollution in developing countries:a major environmental and public health challenge.Bull World Health Organ 78:1078-1092
Brychkova G,Xia Z,Yang G,Yesbergenova Z,Zhang Z,Davydov O,Fluhr R,Sagi M(2007)Sulfite oxidase protects plants against sulfur dioxide toxicity.Plant J 50:696-709
Darrail NM(1989)The effect of air pollutants on physiological processes in plants.Plant Cell Environ 12:1-30
Demmig B,Bj?rkman O(1987)Comparison of the effect of excessive light on chlorophyll fluorescence(77K)and photon yield of O2 evolution in leaves of higher plants.Planta 171:171-184
Draper HH,Hadley M(1990)Malondialdehyde determination as index of lipid peroxidation.Meth Enzymol86:421-431
Ferguson IB,Watkins CB,Harman JE(1983)Inhibition by calcium of senescence of detached cucumber cotyledons.Plant Physiol 71:182-186
Giannopolitis CN,Ries SK(1977)Superoxide dismutase.I.Occurrence in higher plants.Plant Physiol 59:309-314
Jain S,Kumar D,Jain M,Chaudhary P,Deswal R,Sarin NB(2012)Ectopic overexpression of a salt stress-induced pathogenesisrelated class 10 protein(PR10)gene from peanut(Arachis hypogaea L.)affords broad spectrum abiotic stress tolerance in transgenic tobacco.Plant Cell Tiss Organ Cult 109:19-31
Klaff P,Gruissem W(1991)Changes in chloroplast m RNA stability during leaf development.Plant Cell 3:517-529
Klein RR,Mason HS,Mullet JE(1988)Light-regulated translation of chloroplast proteins.I.Transcripts of psa A-psa B,psb A,and rbc L are associated with polysomes in dark-grown and illuminated barley seedlings.J Cell Biol 106:289-301
Klein RR,Mullet JE(1987)Control of gene expression during higher plant chloroplast biogenesis.Protein synthesis and transcript levels of psb A,psa A-psa B,and rbc L in dark-grown and illuminated barley seedlings,J.Biol.Chem.262:4341-4348
Leelavathi S,Bhardwaj A,Kumar S,Dass A,Pathak R,Pandey SS,Tripathy BC,Padmalatha KV,Dhandapani G,Kanakachari M,Solanke AU,Kumar PA,Cella R,Siva Reddy V(2011)Genome-wide transcriptome and proteome analyses of tobacco psa A and psb A deletion mutants.Plant Mol Biol 76:407-423
Livaka KJ,Schmittgen TD(2001)Analysis of relative gene expression data using real-time quantitative PCRand the 2(-Delta Delta C(T))method.Methods 25:402-408
Maxwell K,Johnson GN(2000)Chlorophyll fluorescence-a practical guide.J Exp Bot 51:659-668
Meng Z,Zhang L(1992)Cytogenetic damage induced in human lymphocytes by sodium bisulfite.Mut Res298:63-69
Müller P,Li XP,Niyogi KK(2001)Non-photochemical quenching.A response to excess light energy.Plant Physiol 125:1558-1566
Nishiyama Y,Allakhverdiev SI,Murata N(2006)A new paradigm for the action of reactive oxygen species in the photoinhibition of photosystem II.Biochim Biophys Acta 1757:742-749
Rint?maki E,Kettunen R,Aro EM(1996)Differential D1 dephosphorylation in functional and photodamaged photosystem II centres.J Biol Chem 271:14870-4875
Shen Z,Ding M,Sun J,Deng S,Zhao R,Wang M,Ma X,Wang F,Zhang H,Qian Z,Hu Y,Yu R,Shen X,Chen S(2013)Overexpression of Pe HSF mediates leaf ROS homeostasis in transgenic tobacco lines grown under salt stress conditions.Plant Cell Tiss Organ Cult 115:299-308
Soitama AJ,Zhou G,Clarke AK,Oquist G,Gustafsson P and Aro EM(1996)Over-production of the D1:2protein makes synechococcus cells more tolerant to photoinhibition of photosystem II.Plant Mol Biol 30:467-478
Sun J,Li LS,Liu MQ,Wang MJ,Ding MQ,Deng SR,Lu CF,Zhou XY,Shen X,Zheng XJ,Chen SL(2010)Hydrogen peroxide and nitric oxide mediate K+/Na+homeostasis and antioxidant defense in Na Cl-stressed callus cells of two contrasting poplars.Plant Cell,Tissue Organ Cult 103:205-215
Triolo L,Binazzi A,Cagnetti P,Carconi P,Correnti A,De Luca E,Di Bonito R,Grandoni G,Mastrantonio M,Rosa S,Schimberni M,Uccelli R,Zappa G(2008)Air pollution impact assessment on agro-ecosystem and human health characterization in the area surrounding the industrial settlement of Milazzo:a multidisciplinary approach.Environ Monitor Assess 140:191-209
Xia Z,Su X,Liu J,Wang M(2013)The RING-H2 finger gene 1(RHF1)encodes an E3 ubiquitin ligase and participates in drought stress response in Nicotiana tabacum.Genetica 141:11-21
Xia Z,Sun K,Wang M,Wu K,Zhang H(2012)Overexpression of a maize sulfite oxidase gene in tobacco enhances tolerance to sulfite stress via sulfite oxidation and CAT-mediated H2O2 scavenging.PLo S One 7:e37383
Xuan N,Jin Y,Zhang H,Xie Y,Liu Y,Wang G(2011)A putative maize zinc-finger protein gene,Zm AN13,participates in abiotic stress response.Plant Cell Tiss Organ Cult 107:101-112
Yang C,Deng W,Tang N,Wang X,Yan F,Lin D,Li Z(2013)Overexpression of Zm AFB2,the maize homologue of AFB2 gene,enhances salt tolerance in transgenic tobacco.Plant Cell Tiss Organ Cult 112:171-179
Yarmolinsky D,Brychkova G,Fluhr R,Sagi M(2013)Sulfite reductase protects plants against sulfite toxicity.Plant Physiol 161:725-743