Effect of Dissolved Oxygen Concentrations on Specific Microbial Activities and Their Metabolic Products in Simultaneous Sulfur and Nitrogen Removal
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- 英文论文题名：Effect of Dissolved Oxygen Concentrations on Specific Microbial Activities and Their Metabolic Products in Simultaneous Sulfur and Nitrogen Removal
- 论文作者：W.Thongnueakhaeng ; P.Chaiprasert
- 英文论文作者：W.Thongnueakhaeng ; P.Chaiprasert ; Joint Graduate School of Energy and Environment (JGSEE) ; King Mongkut’s University of Technology Thonburi ; Division of Biotechnology ; School of Bioresources and Technology ; King Mongkut’s University of Technology Thonburi
- 年：2015
- 作者机构：Joint Graduate School of Energy and Environment (JGSEE),King Mongkut’s University of Technology Thonburi;Division of Biotechnology,School of Bioresources and Technology,King Mongkut’s University of Technology Thonburi;
- 英文论文关键词：Simultaneous removal ; sulfate ; ammonium nitrogen ; specific microbial activity ; metabolic product
- 会议召开时间：2015-04-01
- 会议录名称：International Journal of Environmental Science and Development Vol.6 No.4
- 语种：英文
- 分类号：X172;X703
- 学会代码：CDYA
- 学会名称：成都亚昂教育咨询有限公司
- 页数：6
- 文件大小：1288k
- 原文格式：D
摘要
This work investigated simultaneous removal of sulfur and nitrogen compounds under micro oxygen. Different ranges of dissolved oxygen(DO) concentration were used, from 0.05-0.10, 0.10-0.15, 0.15-0.20, 0.20-0.25 and 0.25-0.30 mg/L, to study the effect of DO on specific microbial activities and their metabolic products. The results indicated that the optimal DO concentration was 0.10-0.15 mg/L. This condition provided removal efficiency of SO_4~(2-)-S and NH_4~+-N at 71.2% and 62.8%, respectively. In addition, S~0 and N_2 gas were the required end products for this study. The yield of S~0 and N_2 was 0.63 g-S~0 produced/g-SO_4~(2-)-S added and 0.57 g-N_2 produced/g-NH_4~+-N added, respectively. Activities of sulfate reducing bacteria(SRB), sulfide oxidizing bacteria(SOB), nitrifier and denitrifier were 0.098, 0.361, 0.080 and 0.169 g-substrate consumed/g-VSS/d, respectively. At the lowest DO of 0.05-0.10 mg/L, nitrifier was inhibited, leading to decreasing NH4+-N removal efficiency and N_2 yield. However, sulfate removal and S~0 yield slightly increased. When DO concentrations reached 0.15-0.30 mg/L, sulfate removal efficiency and S~0 yields decreased significantly. In addition, SRB activity was inhibited significantly while activity of SOB was not significantly different. In contrast, the activity of nitrifier was enhanced by increasing oxygen to peak removal of ammonium. However, N_2 gas production was increased slightly because nitrate reduction to N_2 was inhibited at high DO concentrations.
This work investigated simultaneous removal of sulfur and nitrogen compounds under micro oxygen. Different ranges of dissolved oxygen(DO) concentration were used, from 0.05-0.10, 0.10-0.15, 0.15-0.20, 0.20-0.25 and 0.25-0.30 mg/L, to study the effect of DO on specific microbial activities and their metabolic products. The results indicated that the optimal DO concentration was 0.10-0.15 mg/L. This condition provided removal efficiency of SO_4~(2-)-S and NH_4~+-N at 71.2% and 62.8%, respectively. In addition, S~0 and N_2 gas were the required end products for this study. The yield of S~0 and N_2 was 0.63 g-S~0 produced/g-SO_4~(2-)-S added and 0.57 g-N_2 produced/g-NH_4~+-N added, respectively. Activities of sulfate reducing bacteria(SRB), sulfide oxidizing bacteria(SOB), nitrifier and denitrifier were 0.098, 0.361, 0.080 and 0.169 g-substrate consumed/g-VSS/d, respectively. At the lowest DO of 0.05-0.10 mg/L, nitrifier was inhibited, leading to decreasing NH4+-N removal efficiency and N_2 yield. However, sulfate removal and S~0 yield slightly increased. When DO concentrations reached 0.15-0.30 mg/L, sulfate removal efficiency and S~0 yields decreased significantly. In addition, SRB activity was inhibited significantly while activity of SOB was not significantly different. In contrast, the activity of nitrifier was enhanced by increasing oxygen to peak removal of ammonium. However, N_2 gas production was increased slightly because nitrate reduction to N_2 was inhibited at high DO concentrations.
This work investigated simultaneous removal of sulfur and nitrogen compounds under micro oxygen. Different ranges of dissolved oxygen(DO) concentration were used, from 0.05-0.10, 0.10-0.15, 0.15-0.20, 0.20-0.25 and 0.25-0.30 mg/L, to study the effect of DO on specific microbial activities and their metabolic products. The results indicated that the optimal DO concentration was 0.10-0.15 mg/L. This condition provided removal efficiency of SO_4~(2-)-S and NH_4~+-N at 71.2% and 62.8%, respectively. In addition, S~0 and N_2 gas were the required end products for this study. The yield of S~0 and N_2 was 0.63 g-S~0 produced/g-SO_4~(2-)-S added and 0.57 g-N_2 produced/g-NH_4~+-N added, respectively. Activities of sulfate reducing bacteria(SRB), sulfide oxidizing bacteria(SOB), nitrifier and denitrifier were 0.098, 0.361, 0.080 and 0.169 g-substrate consumed/g-VSS/d, respectively. At the lowest DO of 0.05-0.10 mg/L, nitrifier was inhibited, leading to decreasing NH4+-N removal efficiency and N_2 yield. However, sulfate removal and S~0 yield slightly increased. When DO concentrations reached 0.15-0.30 mg/L, sulfate removal efficiency and S~0 yields decreased significantly. In addition, SRB activity was inhibited significantly while activity of SOB was not significantly different. In contrast, the activity of nitrifier was enhanced by increasing oxygen to peak removal of ammonium. However, N_2 gas production was increased slightly because nitrate reduction to N_2 was inhibited at high DO concentrations.
引文
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[9]C.Su,L.Zhu,C.Zhang,X.Qi,Y.Guo,and R.Gao,“Microbial community of aerobic granules for ammonium and sulphide removal in a sequencing batch reactor,”Biotechnol.Lett.,vol.34,pp.883-888,2012.
[10]R.Beristain-Cardoso,D.N.Perez-Gonzalez,G.Gonzalez-Blanco,and J.Gomez,“Simultaneous oxidation of ammonium,p-cresol and sulfide using a nitrifying sludge in a multipurpose bioreactor:A novel alternative,”Bioresource Technology,vol.102,pp.3623-3625,2011.
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[13]C.Rattanapan,D.Kantachote,R.Yan,and P.Boonsawang,“Hydrogen sulfide removal using granular activated carbon biofiltration inoculated with Alcaligenes faecalis T307 isolated from concentrated latex wastewater,”International Biodeterioration and Biodegradation,vol.64,pp.383-387,2010.
[14]A.J.H.Janssen,S.C.Ma,P.Lens,and G.Lettinga,“Performance of a sulfide-oxidizing expanded-bed reactor supplied with dissolved oxygen,”Biotechnol.Bioeng.,vol.53,pp.32-40,1997.
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[17]C.A.Natália,S.A.C.Catarina,M.R.Suzana,A.D.R.José,Z.Marcelo,and F.Eugenio,“Effect of feeding strategy and COD/sulfate ratio on the removal of sulfate in an An SBBR with recirculation of the liquid phase,”Journal of Environmental Management,vol.91,pp.1756-1765,2010.
[18]P.N.L.Lens,A.Visser,A.J.H.Janssen,L.W.Hulshoff Pol,and G.Lettinga,“Biotechnological treatment of sulfate-rich wastewaters,”Critical Reviews in Environmental Science and Technology,vol.28,pp.41-88,1998.
[19]A.H.Nielsen,T.Hvitved-Jacobsen,and J.Vollertsen,“Kinetics and stoichiometry of sulfide oxidation by sewer biofilms,”Water Res.vol.39,pp.4119-4125,2005.
[20]C.Chen,C.Zhou,A.J.Wang,D.H.Wu,L.H.Liu,N.Q.Ren,and D.J.Lee,“Elementary sulfur in effluent from denitrifying sulfide removal process as adsorbent for Zinc(II),”Bioresour.Technol.,vol.121,pp.441-444,2012.
[21]L.B.Celis-Garcia,G.Gonzalez-Blanco,and M.Meraz,“Removal of sulfur inorganic compounds by a biofilm of sulfate reducing and sulfide oxidizing bacteria in a down-flow fluidized bed reactor,”J.Chem.Technol.Biotechnol.,vol.83,pp.260-268,2008.
[22]W.Jianlong,P.Yongzhen,W.Shuying,and G.Yongqing,“Nitrogen Removal by Simultaneous Nitrification and Denitrification via Nitrite in a Sequence Hybrid Biological Reactor,”Chinese Journal of Chemical Engineering,vol.16,no.5,pp.778-784,2008.
[2]A.Sarti,A.J.Silva,M.Zaiat,and E.Foresti,“The treatment of sulfate-rich wastewater using an anaerobic sequencing batch biofilm pilot-scale reactor,”Desalination,vol.249,pp.241-246,2009.
[3]X.Xu,C.Chen,A.Wang,W.Guo,X.Zhou,D.J.Leea,N.Ren,and J.S.Chang,“Simultaneous removal of sulfide,nitrate and acetate under denitrifying sulfide removal condition:Modeling and experimental validation,”Journal of Hazardous Materials,vol.264,2014,pp.16-24.
[4]V.Midha,M.K.Jha,and A.Dey,“Sulfide oxidation in fluidized bed bioreactor using nylon support material,”Journal of Environmental Sciences,vol.24,no.3,pp.512-519,2012.
[5]J.-L.Wang,Y.-Z.Peng,S.-Y.Wang,and Y.-Q.Gao,“Nitrogen removal by simultaneous nitrification and denitrification via nitrite in a sequence hybrid biological reactor,”Chinese Journal of Chemical Engineering,vol.16,no.5,pp.778-784,2008.
[6]X.J.Xu,C.Chen,A.J.Wang,N.Fang,Y.Yuan,N.Ren,and D.J.Lee,“Enhanced elementary sulfur recovery in integrated sulfate-reducing,sulfur-producing rector under micro-aerobic condition,”Bioresource Technology,vol.116,pp.517-521,2012.
[7]J.Reyes-Avila,E.Razo-Floresa,and J.Gomez,“Simultaneous biological removal of nitrogen,carbon and sulfur by denitrification,”Water Research,vol.38,pp.3313-3321,2004.
[8]C.Chen,N.Ren,A.Wang,L.Liu,and D.J.Lee,“Enhanced performance of denitrifying sulfide removal process under micro-aerobic condition,”Journal of Hazardous Materials,vol.179,2010,pp.1147-1151.
[9]C.Su,L.Zhu,C.Zhang,X.Qi,Y.Guo,and R.Gao,“Microbial community of aerobic granules for ammonium and sulphide removal in a sequencing batch reactor,”Biotechnol.Lett.,vol.34,pp.883-888,2012.
[10]R.Beristain-Cardoso,D.N.Perez-Gonzalez,G.Gonzalez-Blanco,and J.Gomez,“Simultaneous oxidation of ammonium,p-cresol and sulfide using a nitrifying sludge in a multipurpose bioreactor:A novel alternative,”Bioresource Technology,vol.102,pp.3623-3625,2011.
[11]APHA,AWWA,WPCF,Standard Methods for Water and Wastewater Examination,21st ed.Washington,DC,USA,2005.
[12]P.N.Lens,M.-P.de Poorter,C.C.Cronenberg,and W.H.Verstraete,“Sulfate reducing and methane producing bacteria in aerobic wastewater treatment systems,”Wat.Res.,vol.29,no.3,pp.871-880,1995.
[13]C.Rattanapan,D.Kantachote,R.Yan,and P.Boonsawang,“Hydrogen sulfide removal using granular activated carbon biofiltration inoculated with Alcaligenes faecalis T307 isolated from concentrated latex wastewater,”International Biodeterioration and Biodegradation,vol.64,pp.383-387,2010.
[14]A.J.H.Janssen,S.C.Ma,P.Lens,and G.Lettinga,“Performance of a sulfide-oxidizing expanded-bed reactor supplied with dissolved oxygen,”Biotechnol.Bioeng.,vol.53,pp.32-40,1997.
[15]L.K.Baumgartner,R.P.Reid,C.Dupraz,A.W.Decho,D.H.Buckley,J.R.Spear,K.M.Przekop,and P.T.Visscher,“Sulfate reducing bacteria in microbial mats:changing paradigms,new discoveries,”Sedimentary Geology,vol.185,pp.131-145,2006.
[16]A.Dolla,M.Fournier,and Z.Dermoun,“Oxygen defense in sulfate-reducing bacteria,”Journal of Biotechnology,vol.126,pp.87-100,2006.
[17]C.A.Natália,S.A.C.Catarina,M.R.Suzana,A.D.R.José,Z.Marcelo,and F.Eugenio,“Effect of feeding strategy and COD/sulfate ratio on the removal of sulfate in an An SBBR with recirculation of the liquid phase,”Journal of Environmental Management,vol.91,pp.1756-1765,2010.
[18]P.N.L.Lens,A.Visser,A.J.H.Janssen,L.W.Hulshoff Pol,and G.Lettinga,“Biotechnological treatment of sulfate-rich wastewaters,”Critical Reviews in Environmental Science and Technology,vol.28,pp.41-88,1998.
[19]A.H.Nielsen,T.Hvitved-Jacobsen,and J.Vollertsen,“Kinetics and stoichiometry of sulfide oxidation by sewer biofilms,”Water Res.vol.39,pp.4119-4125,2005.
[20]C.Chen,C.Zhou,A.J.Wang,D.H.Wu,L.H.Liu,N.Q.Ren,and D.J.Lee,“Elementary sulfur in effluent from denitrifying sulfide removal process as adsorbent for Zinc(II),”Bioresour.Technol.,vol.121,pp.441-444,2012.
[21]L.B.Celis-Garcia,G.Gonzalez-Blanco,and M.Meraz,“Removal of sulfur inorganic compounds by a biofilm of sulfate reducing and sulfide oxidizing bacteria in a down-flow fluidized bed reactor,”J.Chem.Technol.Biotechnol.,vol.83,pp.260-268,2008.
[22]W.Jianlong,P.Yongzhen,W.Shuying,and G.Yongqing,“Nitrogen Removal by Simultaneous Nitrification and Denitrification via Nitrite in a Sequence Hybrid Biological Reactor,”Chinese Journal of Chemical Engineering,vol.16,no.5,pp.778-784,2008.