紫贻贝大防御素在毕赤酵母中的重组DNA表达
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- 英文论文题名：Recombinant DNA expression of big defensin from Mytilusgalloprovincialis in Pichia pastoris
- 论文作者：崔旭 ; 陶妍
- 英文论文作者：CUI Xu ; TAO Yan ; College of Food Science and Technology ; Shanghai Ocean University
- 年：2016
- 作者机构：上海海洋大学食品学院;
- 论文关键词：紫贻贝 ; 大防御素 ; 重组DNA表达 ; 毕赤酵母
- 英文论文关键词：Mytilusgalloprovincialis ; big defensin ; recombinant DNA expression ; Pichia pastoris
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：576k
- 原文格式：D
- 会议级别：全国
摘要
大防御素是一种具有广谱抗菌活性的抗菌肽,是参与生物先天性免疫的关键因子,具有杀灭病菌、保护机体的作用。本研究选取双壳类软体动物紫贻贝的大防御素成熟肽基因mMGBD作为研究对象,根据毕赤酵母的密码子偏爱性对该基因进行优化合成,将其与表达载体pPICZαA连接后电转至毕赤酵母(Pichia pastoris)X-33中,得到的阳性转化子在29℃、经0.75%甲醇诱导表达72小时后,通过SDS-PAGE分析证明重组大防御素rmMGBD得到成功表达。
Big defensin is a kind of antimicrobial peptide which possessesstrong activitiesagainstpathogenic bacteria. In the present study, we focused on the big defensinmature peptide from bivalve mollusk Mytilusgalloprovincialis. A codon-optimized m MGBD gene encoding big defensin mature peptide was synthesized and inserted into the pPICZαA expression vector,and recombinant expression vector pPICZαA-mMGBD was transformed into Pichia pastorisX-33 cells. The recombinant big defensin mature peptide(rm MGBD) was induced with1.0% methanol at 29℃ for 72 h. SDS-PAGE analysis demonstrated thatrm MGBD was expressed successfully.
Big defensin is a kind of antimicrobial peptide which possessesstrong activitiesagainstpathogenic bacteria. In the present study, we focused on the big defensinmature peptide from bivalve mollusk Mytilusgalloprovincialis. A codon-optimized m MGBD gene encoding big defensin mature peptide was synthesized and inserted into the pPICZαA expression vector,and recombinant expression vector pPICZαA-mMGBD was transformed into Pichia pastorisX-33 cells. The recombinant big defensin mature peptide(rm MGBD) was induced with1.0% methanol at 29℃ for 72 h. SDS-PAGE analysis demonstrated thatrm MGBD was expressed successfully.
引文