The virulence and vaccine strains of Streptococcus equi.ssp.zooepidemicushave different influence to phagocytosis and cytokine secretion of macrophage
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- 英文论文题名：The virulence and vaccine strains of Streptococcus equi.ssp.zooepidemicushave different influence to phagocytosis and cytokine secretion of macrophage
- 论文作者：彭婕 ; Ma Zhe ; Hua Chengwei ; Lin Huixing ; Zhang Hui ; Lu Chengping ; Fan Hongjie
- 英文论文作者：Peng Jie ; Ma Zhe ; Hua Chengwei ; Lin Huixing ; Zhang Hui ; Lu Chengping ; Fan Hongjie ; College of Veterinary Medicine ; Nanjing Agricultural University ; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses ; China Animal Health and Epidemiology Center
- 年：2016
- 作者机构：南京农业大学;College of Veterinary Medicine,Nanjing Agricultural University;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses;China Animal Health and Epidemiology Center;
- 英文论文关键词：Streptococcus equi ; Ssp ; Zooepidemicu ; quantitative proteomics ; macrophage ; toxicity difference
- 会议召开时间：2016-09-18
- 会议录名称：第六届中国兽药大会论文集
- 英文会议录名称：Proceedings of 2016 the 6th China Congress of Veterinary Medicine
- 语种：英文
- 分类号：S852.611
- 学会代码：ZGXJ
- 会议名称：第六届中国兽药大会
- 会议地点：中国四川成都
- 主办单位：中国兽医药品监察所、中国兽药协会
- 学会名称：中国畜牧兽医学会
- 页数：2
- 文件大小：131k
- 原文格式：O
- 会议级别：全国
摘要
The swine streptococcosis was a great threat to China pig industry,Streptococcus equi.ssp.zooepidemicus(SEZ) was one of the major pathogens.SEZ ATCC35246 was a classical virulence strain,while SEZ ST171 was an artificial attenuated vaccine strain in China.In this study,we employed the SILAC and LC/MS technology to find out the differentiation in macrophage between these two bacterial infection.87 upregulatedproteins and 135 downregulated proteins were identified.We chose 10 genes for the Real-time PCR and 3 proteins for the Western blot to verify the proteomic results.AU different expression genes were analyzed with R package "clusterProfiler" to get their GO and KEGG informations.Results showed that some of the down regulated genes were associated with immunity functions and up regulated gene were related to cytomembrane and cytoskeleton regulation.Raw264.7 cells phagocytosis rate and cytokine genes transcription to SEZ ATCC35246 and ST171 infection were detected in order to prove bioinformatics conclusion.These results illustrated the different influence to macrophage phagocytosis and cytokine expression might be important reasons of different infection phenotype of SEZ ATCC35246 and ST171.This research willexplainthe mechanism of host immunity responses to SEZ ST171 and how SEZ ATCC35246 invading phagocytosis,provide information on potential therapy and vaccine development targets.
The swine streptococcosis was a great threat to China pig industry,Streptococcus equi.ssp.zooepidemicus(SEZ) was one of the major pathogens.SEZ ATCC35246 was a classical virulence strain,while SEZ ST171 was an artificial attenuated vaccine strain in China.In this study,we employed the SILAC and LC/MS technology to find out the differentiation in macrophage between these two bacterial infection.87 upregulatedproteins and 135 downregulated proteins were identified.We chose 10 genes for the Real-time PCR and 3 proteins for the Western blot to verify the proteomic results.AU different expression genes were analyzed with R package "clusterProfiler" to get their GO and KEGG informations.Results showed that some of the down regulated genes were associated with immunity functions and up regulated gene were related to cytomembrane and cytoskeleton regulation.Raw264.7 cells phagocytosis rate and cytokine genes transcription to SEZ ATCC35246 and ST171 infection were detected in order to prove bioinformatics conclusion.These results illustrated the different influence to macrophage phagocytosis and cytokine expression might be important reasons of different infection phenotype of SEZ ATCC35246 and ST171.This research willexplainthe mechanism of host immunity responses to SEZ ST171 and how SEZ ATCC35246 invading phagocytosis,provide information on potential therapy and vaccine development targets.
The swine streptococcosis was a great threat to China pig industry,Streptococcus equi.ssp.zooepidemicus(SEZ) was one of the major pathogens.SEZ ATCC35246 was a classical virulence strain,while SEZ ST171 was an artificial attenuated vaccine strain in China.In this study,we employed the SILAC and LC/MS technology to find out the differentiation in macrophage between these two bacterial infection.87 upregulatedproteins and 135 downregulated proteins were identified.We chose 10 genes for the Real-time PCR and 3 proteins for the Western blot to verify the proteomic results.AU different expression genes were analyzed with R package "clusterProfiler" to get their GO and KEGG informations.Results showed that some of the down regulated genes were associated with immunity functions and up regulated gene were related to cytomembrane and cytoskeleton regulation.Raw264.7 cells phagocytosis rate and cytokine genes transcription to SEZ ATCC35246 and ST171 infection were detected in order to prove bioinformatics conclusion.These results illustrated the different influence to macrophage phagocytosis and cytokine expression might be important reasons of different infection phenotype of SEZ ATCC35246 and ST171.This research willexplainthe mechanism of host immunity responses to SEZ ST171 and how SEZ ATCC35246 invading phagocytosis,provide information on potential therapy and vaccine development targets.
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