Roles as Potential Oncogene or Tumour Suppressor of the Midclustered MicroRNAs in Gallid Herpesvirus 2(GaHV2)-Induced Marek's Disease Lymphomagenesis
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- 英文论文题名：Roles as Potential Oncogene or Tumour Suppressor of the Midclustered MicroRNAs in Gallid Herpesvirus 2(GaHV2)-Induced Marek's Disease Lymphomagenesis
- 论文作者：Man Teng ; Zu-Hua Yu ; Pu Zhao ; Zi-Xiang Wu ; Lu Dang ; Hui-Zhen Li ; Sheng-Ming Ma ; Gai-Ping Zhang ; 罗俊
- 英文论文作者：Man Teng ; Zu-Hua Yu ; Pu Zhao ; Zi-Xiang Wu ; Lu Dang ; Hui-Zhen Li ; Sheng-Ming Ma ; Gai-Ping Zhang ; Jun Luo ; Key Laboratory of Animal Immunology ; Henan Academy of Agricultural Sciences ; College of Animal Science and Veterinary Medicine ; Henan Agricultural University
- 年：2016
- 作者机构：河南省农业科学院;College of Animal Science and Veterinary Medicine,Henan Agricultural University;
- 英文论文关键词：Herpesvirus ; GaHV2 ; miRNA ; oncogene ; tumour suppressor
- 会议召开时间：2016-09-25
- 会议录名称：第九届全国核糖核酸学术讨论会论文集
- 语种：英文
- 分类号：S857.4
- 学会代码：IGSS
- 会议名称：第九届全国核糖核酸学术讨论会
- 会议地点：中国上海
- 主办单位：中国生物化学与分子生物学会核糖核酸专业委员会
- 学会名称：中国生物化学与分子生物学会
- 页数：1
- 文件大小：391k
- 原文格式：D
- 会议级别：全国
摘要
In the last decade,numerous of miRNAs have been identified in diverse virus families,particularly in herpesviruses.Gallid herpesvirus2(Ga HV2)is a representativeoncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in its natural hosts namely Marek’s disease(MD).In Ga HV2 genome,the presence of26 mature miRNAs derived from 14 precursors assembled in three clusters,namely Meq-cluster,Mid-cluster and LAT-cluster,respectively.Several Ga HV2 miRNAs,especially for those in Meq-cluster(e.g.miR-M4),have been demonstrated to be critical for MD pathogenesis and/or tumourigenesis.The downstream Mid-cluster is regulated and transcribed by a same promoter of Meq-cluster,but the role of Mid-clustered miRNAs in Ga HV2 biology remains unclear.Presently,we have generated Mid-cluster and associated individual miRNA deletion mutants in GX0101 virus,a very virulent(vv)Ga HV2 strain.Using Ga HV2-infected chickens as animal models,we found that deletion of Mid-cluster significantly increased viral pathogenicity as unexpectedly.Compared to parental GX0101 virus,the individual deletion of miR-M1 and miR-M11separately increased the viral pathogenicity or oncogenecity while the individual deletion of miR-M31 significantly decreased the mortality and gross tumour incidence of virus infected chickens.Furthermore,our data confirms that in Ga HV2 infection,the viral oncogene meq is targeted and down-regulated by miR-M11-5p,a miR-M11-derived mature miRNA.To the best of our knowledge,this is the first report to present that the Mid-clustered miRNAs possibly act either as potential oncogene or tumour suppressor in MD lymphomagenesis.
In the last decade,numerous of mi RNAs have been identified in diverse virus families,particularly in herpesviruses.Gallid herpesvirus2(Ga HV2) is a representativeoncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in its natural hosts namely Marek's disease(MD).In Ga HV2 genome,the presence of 26 mature mi RNAs derived from 14 precursors assembled in three clusters,namely Meq-cluster,Mid-cluster and LAT-cluster,respectively.Several Ga HV2 mi RNAs,especially for those in Meq-cluster(e.g.mi R-M4),have been demonstrated to be critical for MD pathogenesis and/or tumourigenesis.The downstream Mid-cluster is regulated and transcribed by a same promoter of Meq-cluster,but the role of Mid-clustered mi RNAs in Ga HV2 biology remains unclear.Presently,we have generated Mid-cluster and associated individual mi RNA deletion mutants in GX0101 virus,a very virulent(vv) Ga HV2 strain.Using Ga HV2-infected chickens as animal models,we found that deletion of Mid-cluster significantly increased viral pathogenicity as unexpectedly.Compared to parental GX0101 virus,the individual deletion of mi R-M1 and mi R-M11 separately increased the viral pathogenicity or oncogenecity while the individual deletion of mi R-M31 significantly decreased the mortality and gross tumour incidence of virus infected chickens.Furthermore,our data confirms that in Ga HV2 infection,the viral oncogene meq is targeted and down-regulated by mi R-M11-5p,a mi R-M11-derived mature mi RNA.To the best of our knowledge,this is the first report to present that the Mid-clustered mi RNAs possibly act either as potential oncogene or tumour suppressor in MD lymphomagenesis.
In the last decade,numerous of mi RNAs have been identified in diverse virus families,particularly in herpesviruses.Gallid herpesvirus2(Ga HV2) is a representativeoncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in its natural hosts namely Marek's disease(MD).In Ga HV2 genome,the presence of 26 mature mi RNAs derived from 14 precursors assembled in three clusters,namely Meq-cluster,Mid-cluster and LAT-cluster,respectively.Several Ga HV2 mi RNAs,especially for those in Meq-cluster(e.g.mi R-M4),have been demonstrated to be critical for MD pathogenesis and/or tumourigenesis.The downstream Mid-cluster is regulated and transcribed by a same promoter of Meq-cluster,but the role of Mid-clustered mi RNAs in Ga HV2 biology remains unclear.Presently,we have generated Mid-cluster and associated individual mi RNA deletion mutants in GX0101 virus,a very virulent(vv) Ga HV2 strain.Using Ga HV2-infected chickens as animal models,we found that deletion of Mid-cluster significantly increased viral pathogenicity as unexpectedly.Compared to parental GX0101 virus,the individual deletion of mi R-M1 and mi R-M11 separately increased the viral pathogenicity or oncogenecity while the individual deletion of mi R-M31 significantly decreased the mortality and gross tumour incidence of virus infected chickens.Furthermore,our data confirms that in Ga HV2 infection,the viral oncogene meq is targeted and down-regulated by mi R-M11-5p,a mi R-M11-derived mature mi RNA.To the best of our knowledge,this is the first report to present that the Mid-clustered mi RNAs possibly act either as potential oncogene or tumour suppressor in MD lymphomagenesis.
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