Responses of AM fungal community to fertilization and plant identity in an alpine meadow on the Qinghai–Tibet Plateau
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- 英文论文题名：Responses of AM fungal community to fertilization and plant identity in an alpine meadow on the Qinghai–Tibet Plateau
- 论文作者：Yong Zheng ; Liang Chen ; Cheng Gao ; Liang-Dong Guo
- 英文论文作者：Yong Zheng ; Liang Chen ; Cheng Gao ; Liang-Dong Guo ; State Key Laboratory of Mycology ; Institute of Microbiology ; Chinese Academy of Sciences
- 年：2016
- 作者机构：State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences;
- 英文论文关键词：454 pyrosequencing ; AM fungi ; community composition ; fertilization ; manure ; nitrogen ; plant identity ; sown pasture
- 会议召开时间：2016-08-19
- 会议录名称：中国菌物学会2016年学术年会论文摘要集
- 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
- 语种：英文
- 分类号：S812
- 学会代码：ZGVL
- 会议名称：中国菌物学会2016年学术年会
- 会议地点：中国福建福州
- 主办单位：中国菌物学会
- 学会名称：中国菌物学会
- 页数：1
- 文件大小：647k
- 原文格式：D
- 会议级别：全国
摘要
Arbuscular mycorrhizal(AM) fungi form symbiotic associations with the majority of land plants and play crucial roles in ecosystems.Understanding the responses of belowground AM fungi in alpine meadow ecosystem facing environmental variation and anthropogenic manipulation may aid sustainable grassland management.Here we studied AM fungal responses to fertilization and plant identity in a natural alpine meadow and a sown pasture on the Qinghai–Tibet Plateau.AM fungal extraradical hyphal(ERH) density was significantly decreased by ammonium nitrogen(NH4+-N) only in May,but was significantly increased by urea and manure under Avena sativa and by manure only under Elymus nutans plantation.AM fungal spore density was not significantly affected by plant identity or fertilizer form,whereas was potentially decreased by higher rate(7.5 g N m-2 yr-1) compared with lower rate(1.5 g N m-2 yr-1) of N addition.Using high-throughput(454) sequencing of 18 S r RNA gene,we observed that AM fungal diversity was significantly higher under E.nutans than Vicia sativa cultivation condition,but AM fungal diversity was not significantly affected by fertilizer urea or sheep manure regardless of plant species.Structural equation model results showed that AM fungal community composition was significantly structured directly by plant identity rather than fertilization,but N rate could strongly affect soil characteristics which subsequently directly influenced community compositions of plants and AM fungi.Taken together,our findings highlight that AM fungal community was influenced by NH4+-N and/or nitrate N(NO3--N) fertilization via mainly altered soil characteristics in the natural alpine meadow,and that plant identity exerts stronger effect than fertilization on soil-dwelling AM fungi in the converted pastureland from the similar alpine meadow.
Arbuscular mycorrhizal(AM) fungi form symbiotic associations with the majority of land plants and play crucial roles in ecosystems.Understanding the responses of belowground AM fungi in alpine meadow ecosystem facing environmental variation and anthropogenic manipulation may aid sustainable grassland management.Here we studied AM fungal responses to fertilization and plant identity in a natural alpine meadow and a sown pasture on the Qinghai–Tibet Plateau.AM fungal extraradical hyphal(ERH) density was significantly decreased by ammonium nitrogen(NH4+-N) only in May,but was significantly increased by urea and manure under Avena sativa and by manure only under Elymus nutans plantation.AM fungal spore density was not significantly affected by plant identity or fertilizer form,whereas was potentially decreased by higher rate(7.5 g N m-2 yr-1) compared with lower rate(1.5 g N m-2 yr-1) of N addition.Using high-throughput(454) sequencing of 18 S r RNA gene,we observed that AM fungal diversity was significantly higher under E.nutans than Vicia sativa cultivation condition,but AM fungal diversity was not significantly affected by fertilizer urea or sheep manure regardless of plant species.Structural equation model results showed that AM fungal community composition was significantly structured directly by plant identity rather than fertilization,but N rate could strongly affect soil characteristics which subsequently directly influenced community compositions of plants and AM fungi.Taken together,our findings highlight that AM fungal community was influenced by NH4+-N and/or nitrate N(NO3--N) fertilization via mainly altered soil characteristics in the natural alpine meadow,and that plant identity exerts stronger effect than fertilization on soil-dwelling AM fungi in the converted pastureland from the similar alpine meadow.
Arbuscular mycorrhizal(AM) fungi form symbiotic associations with the majority of land plants and play crucial roles in ecosystems.Understanding the responses of belowground AM fungi in alpine meadow ecosystem facing environmental variation and anthropogenic manipulation may aid sustainable grassland management.Here we studied AM fungal responses to fertilization and plant identity in a natural alpine meadow and a sown pasture on the Qinghai–Tibet Plateau.AM fungal extraradical hyphal(ERH) density was significantly decreased by ammonium nitrogen(NH4+-N) only in May,but was significantly increased by urea and manure under Avena sativa and by manure only under Elymus nutans plantation.AM fungal spore density was not significantly affected by plant identity or fertilizer form,whereas was potentially decreased by higher rate(7.5 g N m-2 yr-1) compared with lower rate(1.5 g N m-2 yr-1) of N addition.Using high-throughput(454) sequencing of 18 S r RNA gene,we observed that AM fungal diversity was significantly higher under E.nutans than Vicia sativa cultivation condition,but AM fungal diversity was not significantly affected by fertilizer urea or sheep manure regardless of plant species.Structural equation model results showed that AM fungal community composition was significantly structured directly by plant identity rather than fertilization,but N rate could strongly affect soil characteristics which subsequently directly influenced community compositions of plants and AM fungi.Taken together,our findings highlight that AM fungal community was influenced by NH4+-N and/or nitrate N(NO3--N) fertilization via mainly altered soil characteristics in the natural alpine meadow,and that plant identity exerts stronger effect than fertilization on soil-dwelling AM fungi in the converted pastureland from the similar alpine meadow.
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