Phytophthora sojae Avirulence Effector PsAvr3c Target Soybean Serine and Arginine Rich Proteins SRKPs,a Novel Component of Alternative Splicing Complex,to Regulate Plant Immunity
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- 英文论文题名：Phytophthora sojae Avirulence Effector PsAvr3c Target Soybean Serine and Arginine Rich Proteins SRKPs,a Novel Component of Alternative Splicing Complex,to Regulate Plant Immunity
- 论文作者：HUANG Jie ; KONG Guang-hui ; YAN Ting-xiu ; HANG Yu ; ZHANG Ying ; QIU Min ; GUO Bao-dian ; KONG Liang ; JING Mao-feng ; Mark Gijzen ; WANG Yuan-chao ; DONG Suo-meng
- 英文论文作者：HUANG Jie ; KONG Guang-hui ; YAN Ting-xiu ; HANG Yu ; ZHANG Ying ; QIU Min ; GUO Bao-dian ; KONG Liang ; JING Mao-feng ; Mark Gijzen ; WANG Yuan-chao ; DONG Suo-meng ; Department of Plant Pathology ; Nanjing Agricultural University ; Southern crop protection and food research center ; Agricultural and Agri-Food Canada
- 年：2016
- 作者机构：Department of Plant Pathology,Nanjing Agricultural University;Southern crop protection and food research center,Agricultural and Agri-Food Canada;
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S435.651
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：43k
- 原文格式：O
- 会议级别：全国
摘要
Avirulence(AVR) effectors,recognized by host resistant protein,are a group of key players in plant-microbe interactions.However,how AVR effectors manipulate plant immunity remains poorly characterized.The AVR effector PsAvr3 c from soybean(Glycine max) root rot pathogen Phytophthora sojae is recognized by the soybean Rps3 c receptor in a nuclear dependent manner.PsAvr3 c also functions as a virulence protein to enhance Phytophthora colonization.Recendy,we identified two soybean serine/arginine rich proteins GmSRKP1 and GmSRKP2 as interactors of PsAvr3 c.We demonstrated that PsAvr3 c physically interacts with GmSRKP1/2 and stabilizes GmSRKPs proteins in vivo.The GmSRKPs are conserved proteins in plants without any predicted functional domains other than three serine/arginine/lysine rich repeat regions and a nuclear localization signal.Transient gene silencing and over-expression of GmSRKP1/2 in soybean hairy roots indicates that GmSRKP1/2 are susceptibility factors.Furthermore,biochemical assays suggest that GmSRKPs possess RNA binding activity in vitro,and GmSRKPs also associated with some known alternative splicing factors.In summary,these findings provide evidence that a pathogen AVR effector targets a novel component of alternative splicing complex to subvert plant immunity.
Avirulence(AVR) effectors,recognized by host resistant protein,are a group of key players in plant-microbe interactions.However,how AVR effectors manipulate plant immunity remains poorly characterized.The AVR effector PsAvr3 c from soybean(Glycine max) root rot pathogen Phytophthora sojae is recognized by the soybean Rps3 c receptor in a nuclear dependent manner.PsAvr3 c also functions as a virulence protein to enhance Phytophthora colonization.Recendy,we identified two soybean serine/arginine rich proteins GmSRKP1 and GmSRKP2 as interactors of PsAvr3 c.We demonstrated that PsAvr3 c physically interacts with GmSRKP1/2 and stabilizes GmSRKPs proteins in vivo.The GmSRKPs are conserved proteins in plants without any predicted functional domains other than three serine/arginine/lysine rich repeat regions and a nuclear localization signal.Transient gene silencing and over-expression of GmSRKP1/2 in soybean hairy roots indicates that GmSRKP1/2 are susceptibility factors.Furthermore,biochemical assays suggest that GmSRKPs possess RNA binding activity in vitro,and GmSRKPs also associated with some known alternative splicing factors.In summary,these findings provide evidence that a pathogen AVR effector targets a novel component of alternative splicing complex to subvert plant immunity.
Avirulence(AVR) effectors,recognized by host resistant protein,are a group of key players in plant-microbe interactions.However,how AVR effectors manipulate plant immunity remains poorly characterized.The AVR effector PsAvr3 c from soybean(Glycine max) root rot pathogen Phytophthora sojae is recognized by the soybean Rps3 c receptor in a nuclear dependent manner.PsAvr3 c also functions as a virulence protein to enhance Phytophthora colonization.Recendy,we identified two soybean serine/arginine rich proteins GmSRKP1 and GmSRKP2 as interactors of PsAvr3 c.We demonstrated that PsAvr3 c physically interacts with GmSRKP1/2 and stabilizes GmSRKPs proteins in vivo.The GmSRKPs are conserved proteins in plants without any predicted functional domains other than three serine/arginine/lysine rich repeat regions and a nuclear localization signal.Transient gene silencing and over-expression of GmSRKP1/2 in soybean hairy roots indicates that GmSRKP1/2 are susceptibility factors.Furthermore,biochemical assays suggest that GmSRKPs possess RNA binding activity in vitro,and GmSRKPs also associated with some known alternative splicing factors.In summary,these findings provide evidence that a pathogen AVR effector targets a novel component of alternative splicing complex to subvert plant immunity.
引文