Genetic Polymorphisms,Messenger RNA Expression of p53,p21,and MDM2,and Possible Links with Cholinesterase activities in Omethoate-Exposed Workers
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- 英文论文题名：Genetic Polymorphisms,Messenger RNA Expression of p53,p21,and MDM2,and Possible Links with Cholinesterase activities in Omethoate-Exposed Workers
- 论文作者：Mingcui Ding ; Wei Wang
- 英文论文作者：Mingcui Ding ; Wei Wang ; Department of Occupational Health and Occupational Disease ; College of Public Health ; Zhengzhou University
- 年：2016
- 作者机构：Department of Occupational Health and Occupational Disease,College of Public Health,Zhengzhou University;
- 英文论文关键词：Omethoate ; Cholinesterase ; Genetic Polymorphism
- 会议召开时间：2016-11-16
- 会议录名称：第六届中国毒理学会免疫毒理专业委员会换届及学术会议资料汇编
- 语种：英文
- 分类号：R13
- 学会代码：ZGDV
- 会议名称：第六届中国毒理学会免疫毒理专业委员会换届及学术会议
- 会议地点：中国河南开封
- 主办单位：中国毒理学会免疫毒理专业委员会
- 学会名称：中国毒理学会
- 页数：1
- 文件大小：62k
- 原文格式：O
- 会议级别：全国
摘要
Purpose:To explore the influencing factors of cholinesterase activity in omethoate-exposed population and screen susceptible people.Methods:To detect the cholinesterase activities in whole blood,red blood cell and plasma using the methods of GBZ52-2002 appendix B;Genetic Polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method,and real-time PCR method was applied to determine gene expression levels.Results:The cholinesterase activities of whole blood,red blood cells and plasma in exposure group were lower than those of the control group,the differences had statistical significance(P< 0.001);the mRNA expression levels of p53 and p21 in exposure group were significantly lower than those of the control group(Z=12.137,P<0.001;Z=5.358,P<0.001);Multivariate analysis found that group(b=-1.109,P<0.001),gender(b= 0.379,P<0.001),alcohol(6=0.228,P=0.027) and p53 rs 17878362(b=-0.400,P=0.048) had an impact on cholinesterase activities;However,smoking,age,working duration and other genetic polymorphisms were not found to have impacts on it(P>0.05).Conclusions:The whole blood,RBC and plasma cholinesterase activities in exposure group were lower than those of the control group,and the main influencing factors were omethoate-exposure,gender,age,alcohol and p53 rs 17878362 polymorphism;moreover,the cholinesterase activities of LL+SL genotypes individuals for p53 rs 17878362 genetic polymorphism were significantly lower than those of SS genotype,suggesting that SS genotype may be the protective factor of cholinesterase.
Purpose:To explore the influencing factors of cholinesterase activity in omethoate-exposed population and screen susceptible people.Methods:To detect the cholinesterase activities in whole blood,red blood cell and plasma using the methods of GBZ52-2002 appendix B;Genetic Polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method,and real-time PCR method was applied to determine gene expression levels.Results:The cholinesterase activities of whole blood,red blood cells and plasma in exposure group were lower than those of the control group,the differences had statistical significance(P< 0.001);the mRNA expression levels of p53 and p21 in exposure group were significantly lower than those of the control group(Z=12.137,P<0.001;Z=5.358,P<0.001);Multivariate analysis found that group(b=-1.109,P<0.001),gender(b= 0.379,P<0.001),alcohol(6=0.228,P=0.027) and p53 rs 17878362(b=-0.400,P=0.048) had an impact on cholinesterase activities;However,smoking,age,working duration and other genetic polymorphisms were not found to have impacts on it(P>0.05).Conclusions:The whole blood,RBC and plasma cholinesterase activities in exposure group were lower than those of the control group,and the main influencing factors were omethoate-exposure,gender,age,alcohol and p53 rs 17878362 polymorphism;moreover,the cholinesterase activities of LL+SL genotypes individuals for p53 rs 17878362 genetic polymorphism were significantly lower than those of SS genotype,suggesting that SS genotype may be the protective factor of cholinesterase.
Purpose:To explore the influencing factors of cholinesterase activity in omethoate-exposed population and screen susceptible people.Methods:To detect the cholinesterase activities in whole blood,red blood cell and plasma using the methods of GBZ52-2002 appendix B;Genetic Polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method,and real-time PCR method was applied to determine gene expression levels.Results:The cholinesterase activities of whole blood,red blood cells and plasma in exposure group were lower than those of the control group,the differences had statistical significance(P< 0.001);the mRNA expression levels of p53 and p21 in exposure group were significantly lower than those of the control group(Z=12.137,P<0.001;Z=5.358,P<0.001);Multivariate analysis found that group(b=-1.109,P<0.001),gender(b= 0.379,P<0.001),alcohol(6=0.228,P=0.027) and p53 rs 17878362(b=-0.400,P=0.048) had an impact on cholinesterase activities;However,smoking,age,working duration and other genetic polymorphisms were not found to have impacts on it(P>0.05).Conclusions:The whole blood,RBC and plasma cholinesterase activities in exposure group were lower than those of the control group,and the main influencing factors were omethoate-exposure,gender,age,alcohol and p53 rs 17878362 polymorphism;moreover,the cholinesterase activities of LL+SL genotypes individuals for p53 rs 17878362 genetic polymorphism were significantly lower than those of SS genotype,suggesting that SS genotype may be the protective factor of cholinesterase.
引文