八钼氧酸盐复合材料去除人血浆中高丰度蛋白
详细信息 查看官网全文
- 英文论文题名：Depletion of High Abundant Proteins in Human Plasma using Octamolybdate-based Composite
- 论文作者：陈晴 ; 胡雪 ; 陈旭伟 ; 王建华
- 英文论文作者：Qing Chen ; Xue Hu ; Xuwei Chen ; Jianhua Wang ; Research Center for Analytical Sciences ; Northeastern University
- 年：2016
- 作者机构：东北大学分析科学研究中心;
- 论文关键词：多金属氧簇 ; 八钼氧酸盐 ; HSA ; IgG ; 蛋白质去除
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十三分会：复杂样品分离分析
- 语种：中文
- 分类号：R446.1;TB33
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十三分会 ; 复杂样品分离分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：136k
- 原文格式：D
- 会议级别：全国
摘要
通过静电作用将多金属氧簇八钼氧酸盐[(C_(16)H_(36)BrN)_4Mo_8O_(26),(Mo_8O_(26))]与多孔金属有机骨架MIL-101(Cr)相结合,制备了新型的复合材料Mo_8O_(26)@MIL-101。采用XRD、TG、FT-IR、Raman、SEM、EDS和BET等技术手段对该材料进行了表征,所得材料性质稳定,比表面积高达1499m~2/g。基于Mo_8O_(26)~(4-)与组氨酸之间的共价配位作用,该复合材料对高丰度组氨酸蛋白HSA和IgG表现出良好的吸附选择性。在pH=5.0的条件下,对HSA和IgG的吸附容量分别为785.6 mg/g和420.1 mg/g。据此建立了固相吸附过程用于蛋白质的吸附与去除。SDS-PAGE(Fig.1)和2D-PAGE(Fig.2)电泳结果表明,人血浆样品经Mo_8O_(26)@MIL-101吸附处理后,高丰度HSA和IgG得到有效去除;去除后的血浆样品经LC-MS/MS可鉴定出334种血浆蛋白,其中中低丰度蛋白占蛋白总数的53.66%。
A novel composite,namely Mo_8O_(26)@MIL-101,is prepared by the electrostatic binding of octamolybdate(Mo_8O_(26)) with the porous Metal organic framework MIL-101(Cr).The obtained composite owns a surface area of 1499m~2/g.Due to the coordination between the Mo_8O_(26)~(4-) and histidine,the composite exhibits excellent adsorption towards HSA and IgG.At pH=5.0,the adsorption capacities for HSA and IgG is 785.6 mg g~(-1)and 420.1 mg g~(-1).SDS-PAGE and 2D-PAGE results indicate that efficient removal of abundant HSA and IgG from plasma is achieved.A total of 334 plasma proteins could be identified from the treated plasma by LC-MS/MS and the percentage of proteins of middle and low abundance is 53.66%.
A novel composite,namely Mo_8O_(26)@MIL-101,is prepared by the electrostatic binding of octamolybdate(Mo_8O_(26)) with the porous Metal organic framework MIL-101(Cr).The obtained composite owns a surface area of 1499m~2/g.Due to the coordination between the Mo_8O_(26)~(4-) and histidine,the composite exhibits excellent adsorption towards HSA and IgG.At pH=5.0,the adsorption capacities for HSA and IgG is 785.6 mg g~(-1)and 420.1 mg g~(-1).SDS-PAGE and 2D-PAGE results indicate that efficient removal of abundant HSA and IgG from plasma is achieved.A total of 334 plasma proteins could be identified from the treated plasma by LC-MS/MS and the percentage of proteins of middle and low abundance is 53.66%.
引文
[1]Ferey,G.;Mellot-Draznieks,C.;Serre,C.;Millange,F.;Dutour,J.;Surble,S.;Margiolaki,I.Science 2005,309:4020.
[2]Kowalewski,B.;Poppe,J.;Demmer,U.;Warkentin,E.;Dierks,T.;Ermler,U.;Schneider,K.J.Am.Chem.Soc.2012,134:9768.
[2]Kowalewski,B.;Poppe,J.;Demmer,U.;Warkentin,E.;Dierks,T.;Ermler,U.;Schneider,K.J.Am.Chem.Soc.2012,134:9768.