Identification of sex-related genes based on expressed sequence tag analysis of cDNA libraries of adult Toxocara canis
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- 英文论文题名：Identification of sex-related genes based on expressed sequence tag analysis of cDNA libraries of adult Toxocara canis
- 论文作者：Zhou rongqiong ; Ma guangxu ; Zhu honghong ; Luo yongfang ; Luo yongli ; Yin shasha ; Xiong yi
- 英文论文作者：Zhou rongqiong ; Ma guangxu ; Zhu honghong ; Luo yongfang ; Luo yongli ; Yin shasha ; Xiong yi ; Department of Veterinary Medicine ; Rongchang Campus ; Southwest University
- 年：2016
- 作者机构：Department of Veterinary Medicine,Rongchang Campus,Southwest University;
- 英文论文关键词：Toxocara canis ; Sex-related genes ; Expressed sequence tags(ESTs) ; Iden-tification
- 会议召开时间：2016-05-25
- 会议录名称：第七届中国畜牧科技论坛论文集
- 英文会议录名称：Proceeding of the 7th China Animal Husbandry Sci-tech Forum
- 语种：英文
- 分类号：S852.7
- 学会代码：ZGXJ
- 会议名称：第七届中国畜牧科技论坛
- 会议地点：中国重庆
- 主办单位：中国畜牧兽医学会
- 学会名称：中国畜牧兽医学会
- 页数：2
- 文件大小：106k
- 原文格式：O
- 会议级别：全国
摘要
The nematode Toxocara canis is a cosmopolitan parasite of canids with a broad paratenic host range that includes humans.We generated two libraries from male and female adult T.canis,and analyzed the expressed sequence tags(ESTs) to identify genes involved in sexual determination,reproduction,and development.A total of 4328 clones were sequenced,yielding 3803 ESTs with an average sequence length of 688 bp.The cluster and assembly analysis yielded 1027 unique sequences that included 323 contigs and 704 singletons.BLASTX searches revealed 384 unigenes that had no significant matches to published sequences,which comprised 37.39%of the total number of sequences.The remaining 643 unigenes(62.61%)shared high levels of sequence identity with published nucleotide or amino-acid sequences.Based on overall EST abundance,the major sex-related genes were major sperm protein isoform beta(329,13.57%) and immunosuppressive ovarian message protein(45,3.27%).Through comparisons with data from previous studies,33 sex-related genes were identified,which comprised 3.21%of the total unique cDNAs.The transcription profiles of ten of these sex-related gene homologs were examined in male and female adult T.canis using reverse transcription and real-time polymerase chain reaction.Our findings will aid future investigations of sex-related gene expression in T.canis,and provide insight into novel molecular targets for the control of the parasite.
The nematode Toxocara canis is a cosmopolitan parasite of canids with a broad paratenic host range that includes humans.We generated two libraries from male and female adult T.canis,and analyzed the expressed sequence tags(ESTs) to identify genes involved in sexual determination,reproduction,and development.A total of 4328 clones were sequenced,yielding 3803 ESTs with an average sequence length of 688 bp.The cluster and assembly analysis yielded 1027 unique sequences that included 323 contigs and 704 singletons.BLASTX searches revealed 384 unigenes that had no significant matches to published sequences,which comprised 37.39%of the total number of sequences.The remaining 643 unigenes(62.61%)shared high levels of sequence identity with published nucleotide or amino-acid sequences.Based on overall EST abundance,the major sex-related genes were major sperm protein isoform beta(329,13.57%) and immunosuppressive ovarian message protein(45,3.27%).Through comparisons with data from previous studies,33 sex-related genes were identified,which comprised 3.21%of the total unique cDNAs.The transcription profiles of ten of these sex-related gene homologs were examined in male and female adult T.canis using reverse transcription and real-time polymerase chain reaction.Our findings will aid future investigations of sex-related gene expression in T.canis,and provide insight into novel molecular targets for the control of the parasite.
The nematode Toxocara canis is a cosmopolitan parasite of canids with a broad paratenic host range that includes humans.We generated two libraries from male and female adult T.canis,and analyzed the expressed sequence tags(ESTs) to identify genes involved in sexual determination,reproduction,and development.A total of 4328 clones were sequenced,yielding 3803 ESTs with an average sequence length of 688 bp.The cluster and assembly analysis yielded 1027 unique sequences that included 323 contigs and 704 singletons.BLASTX searches revealed 384 unigenes that had no significant matches to published sequences,which comprised 37.39%of the total number of sequences.The remaining 643 unigenes(62.61%)shared high levels of sequence identity with published nucleotide or amino-acid sequences.Based on overall EST abundance,the major sex-related genes were major sperm protein isoform beta(329,13.57%) and immunosuppressive ovarian message protein(45,3.27%).Through comparisons with data from previous studies,33 sex-related genes were identified,which comprised 3.21%of the total unique cDNAs.The transcription profiles of ten of these sex-related gene homologs were examined in male and female adult T.canis using reverse transcription and real-time polymerase chain reaction.Our findings will aid future investigations of sex-related gene expression in T.canis,and provide insight into novel molecular targets for the control of the parasite.
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