Polylysine-modified DNA vaccine showed elevated prophylactic and therapeutic effects in peanut allergy in mice
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- 英文论文题名：Polylysine-modified DNA vaccine showed elevated prophylactic and therapeutic effects in peanut allergy in mice
- 论文作者：Zhu Zhenlai ; Sun Shuhong ; Niu Yuan ; Li Wei
- 英文论文作者：Zhu Zhenlai ; Sun Shuhong ; Niu Yuan ; Li Wei ; Department of Dermatology ; Xijing Hospital ; Fourth Military Medical University
- 年：2016
- 作者机构：Department of Dermatology,Xijing Hospital,Fourth Military Medical University;
- 英文论文关键词：Ara h 2 ; Allergy vaccines ; Peanut allergy ; Polylysine ; Regulatory T cells
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会壁报交流集
- 英文会议录名称：Proceedings of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R392
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1353k
- 原文格式：D
- 会议级别：全国
摘要
Objective:The prevalence of food allergy has been increasing,but the treatment is very limited.DNA vaccination hasbeen recognized as a promising method for the treatment of allergic diseases;however,poor immunogenicity has hindered its application.In the present study,we assessed the prophylactic and therapeutic effects of poly-L-lysine(PLL)-pretreated DNA vaccine encoding the major peanut allergen Ara h 2 in mice.Methods:BALB/c mice were intradermally injected with plasmid DNA encoding the peanut protein Ara h 2(p Ara h 2) or p Ara h 2 pre-treated with PLL before or after sensitization with Ara h 2 protein.Ara h 2-specific antibodies were measured by ELISA.Langerhans cells(LCs) and Treg cells in draining lymph nodes were analyzed by flow cytometry after DNA immunization,and IL-10 production in splenocytes was also analyzed.Results:In the prophylactic study,pre-treatment with p Ara h 2 or PLL-p Ara h 2 resulted in lower level of Ara h 2-specific Ig G1,Ig G2 a and Ig E after sensitization with Ara h 2 protein,and mice in PLL-p Ara h 2 group showed significantly lower level of antibodies than that of p Ara h 2.In the treatment study,intradermal injection with p Ara h 2 or PLL-p Ara h 2 after Ara h 2 protein sensitization significantly decreased the level of Ara h 2-specific antibodies,and PLL-p Ara h 2 had stronger effects than p Ara h 2.There were increased numbers of LCs and Treg cells in the mice receiving intradermal injection with PLL-p Ara h 2,and splenocytes from PLL-p Ara h 2-treated mice secreted increased level of IL-10.Conclusions:Modification of pA ra h 2 with PLL improved its prophylactic and therapeutic effects in peanut allergy in mice.
Objective:The prevalence of food allergy has been increasing,but the treatment is very limited.DNA vaccination hasbeen recognized as a promising method for the treatment of allergic diseases;however,poor immunogenicity has hindered its application.In the present study,we assessed the prophylactic and therapeutic effects of poly-L-lysine(PLL)-pretreated DNA vaccine encoding the major peanut allergen Ara h 2 in mice.Methods:BALB/c mice were intradermally injected with plasmid DNA encoding the peanut protein Ara h 2(p Ara h 2) or p Ara h 2 pre-treated with PLL before or after sensitization with Ara h 2 protein.Ara h 2-specific antibodies were measured by ELISA.Langerhans cells(LCs) and Treg cells in draining lymph nodes were analyzed by flow cytometry after DNA immunization,and IL-10 production in splenocytes was also analyzed.Results:In the prophylactic study,pre-treatment with p Ara h 2 or PLL-p Ara h 2 resulted in lower level of Ara h 2-specific Ig G1,Ig G2 a and Ig E after sensitization with Ara h 2 protein,and mice in PLL-p Ara h 2 group showed significantly lower level of antibodies than that of p Ara h 2.In the treatment study,intradermal injection with p Ara h 2 or PLL-p Ara h 2 after Ara h 2 protein sensitization significantly decreased the level of Ara h 2-specific antibodies,and PLL-p Ara h 2 had stronger effects than p Ara h 2.There were increased numbers of LCs and Treg cells in the mice receiving intradermal injection with PLL-p Ara h 2,and splenocytes from PLL-p Ara h 2-treated mice secreted increased level of IL-10.Conclusions:Modification of pA ra h 2 with PLL improved its prophylactic and therapeutic effects in peanut allergy in mice.
Objective:The prevalence of food allergy has been increasing,but the treatment is very limited.DNA vaccination hasbeen recognized as a promising method for the treatment of allergic diseases;however,poor immunogenicity has hindered its application.In the present study,we assessed the prophylactic and therapeutic effects of poly-L-lysine(PLL)-pretreated DNA vaccine encoding the major peanut allergen Ara h 2 in mice.Methods:BALB/c mice were intradermally injected with plasmid DNA encoding the peanut protein Ara h 2(p Ara h 2) or p Ara h 2 pre-treated with PLL before or after sensitization with Ara h 2 protein.Ara h 2-specific antibodies were measured by ELISA.Langerhans cells(LCs) and Treg cells in draining lymph nodes were analyzed by flow cytometry after DNA immunization,and IL-10 production in splenocytes was also analyzed.Results:In the prophylactic study,pre-treatment with p Ara h 2 or PLL-p Ara h 2 resulted in lower level of Ara h 2-specific Ig G1,Ig G2 a and Ig E after sensitization with Ara h 2 protein,and mice in PLL-p Ara h 2 group showed significantly lower level of antibodies than that of p Ara h 2.In the treatment study,intradermal injection with p Ara h 2 or PLL-p Ara h 2 after Ara h 2 protein sensitization significantly decreased the level of Ara h 2-specific antibodies,and PLL-p Ara h 2 had stronger effects than p Ara h 2.There were increased numbers of LCs and Treg cells in the mice receiving intradermal injection with PLL-p Ara h 2,and splenocytes from PLL-p Ara h 2-treated mice secreted increased level of IL-10.Conclusions:Modification of pA ra h 2 with PLL improved its prophylactic and therapeutic effects in peanut allergy in mice.
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