Precocious line selection of Eimeria tenella isolated from Hebei, China
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- 英文论文题名：Precocious line selection of Eimeria tenella isolated from Hebei, China
- 论文作者：Xiaoli Tang ; Guangping Huang ; Xianyong Liu ; Xun Suo
- 英文论文作者：Xiaoli Tang ; Guangping Huang ; Xianyong Liu ; Xun Suo ; National Animal Protozoal laboratory & College of Veterinary Medicine ; China Agricultural University
- 年：2016
- 作者机构：National Animal Protozoal laboratory & College of Veterinary Medicine, China Agricultural University;
- 英文论文关键词：Eimeria tenella ; precocious line selection ; China
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：S852.723
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：155k
- 原文格式：D
- 会议级别：全国
摘要
Introduction Avian coccidiosis, a protozoan disease, which caused by Eimeria species is considered of worldwide economically important disease for poultry farm. Given the drug resistance, vaccination with attenuated lines of the parasites is rapidly gaining great prominence in commercial poultry production. Attenuated vaccine lines of Eimeria have been developed widely in Britain, USA and Spain. Until recently, however, there have been no attenuated coccidiosis vaccines available in China. E. tenella capable of causing haemorrhage in the caecum of the fowl is now recognized as a significant pathogen. All the facts have stimulated the selection of precocious line of E. tenella, which will be more efficient for preventing coccidiosis in China. Material & methods All equipment and facilities including isolators and cages were cleaned and sterilised using ammonia for at least one night before use. All feed was formulated with no coccidiostat. Feed and water were stored at 80°C for 2 hours at least to avoid contamination. The original parent line of E. tenella, came from Hebei province of China, was cultivated from a single oocyst and the pathogenicity assay was conducted. The precocious line was obtained by repeatedly selecting early development of oocysts. Each passage was propagated by orally administering oocysts to coccidia-free chickens, and the prepatent period was detected. Oocysts were harvested from faeces a few hours later. Whenever possible, the selection pressure was applied during selection for precociousness. The mean dimensions of sporulated oocysts of the 10 th generation and parent strains were measured to determine if selection for precociousness resulted in a change in oocyst size. 100 randomly encountered oocysts were measured on the photographs pictured by microscope. Results The parent line was identified as a low virulence strain because no bird died even the inoculation dose was 1×106 sporulated oocysts. Selection for precociousness was effective in reducing the prepatent period by 11 hours through 14 generations. What's more, a significant difference in the oocysts dimensions between parent line and 10 generations of selection appeared. The mean size of sporulated oocysts of the parent line and 10 th generations of the precocious line are 22.42×17.79 and 20.14×16.76, respectively. Conclusion The results show that after pressure selection the line in the present study has possessed precocious characters. However, the selection of precocious line should be continued to obtain genetically stable strain.
Introduction Avian coccidiosis, a protozoan disease, which caused by Eimeria species is considered of worldwide economically important disease for poultry farm. Given the drug resistance, vaccination with attenuated lines of the parasites is rapidly gaining great prominence in commercial poultry production. Attenuated vaccine lines of Eimeria have been developed widely in Britain, USA and Spain. Until recently, however, there have been no attenuated coccidiosis vaccines available in China. E. tenella capable of causing haemorrhage in the caecum of the fowl is now recognized as a significant pathogen. All the facts have stimulated the selection of precocious line of E. tenella, which will be more efficient for preventing coccidiosis in China. Material & methods All equipment and facilities including isolators and cages were cleaned and sterilised using ammonia for at least one night before use. All feed was formulated with no coccidiostat. Feed and water were stored at 80°C for 2 hours at least to avoid contamination. The original parent line of E. tenella, came from Hebei province of China, was cultivated from a single oocyst and the pathogenicity assay was conducted. The precocious line was obtained by repeatedly selecting early development of oocysts. Each passage was propagated by orally administering oocysts to coccidia-free chickens, and the prepatent period was detected. Oocysts were harvested from faeces a few hours later. Whenever possible, the selection pressure was applied during selection for precociousness. The mean dimensions of sporulated oocysts of the 10 th generation and parent strains were measured to determine if selection for precociousness resulted in a change in oocyst size. 100 randomly encountered oocysts were measured on the photographs pictured by microscope. Results The parent line was identified as a low virulence strain because no bird died even the inoculation dose was 1×106 sporulated oocysts. Selection for precociousness was effective in reducing the prepatent period by 11 hours through 14 generations. What's more, a significant difference in the oocysts dimensions between parent line and 10 generations of selection appeared. The mean size of sporulated oocysts of the parent line and 10 th generations of the precocious line are 22.42×17.79 and 20.14×16.76, respectively. Conclusion The results show that after pressure selection the line in the present study has possessed precocious characters. However, the selection of precocious line should be continued to obtain genetically stable strain.
Introduction Avian coccidiosis, a protozoan disease, which caused by Eimeria species is considered of worldwide economically important disease for poultry farm. Given the drug resistance, vaccination with attenuated lines of the parasites is rapidly gaining great prominence in commercial poultry production. Attenuated vaccine lines of Eimeria have been developed widely in Britain, USA and Spain. Until recently, however, there have been no attenuated coccidiosis vaccines available in China. E. tenella capable of causing haemorrhage in the caecum of the fowl is now recognized as a significant pathogen. All the facts have stimulated the selection of precocious line of E. tenella, which will be more efficient for preventing coccidiosis in China. Material & methods All equipment and facilities including isolators and cages were cleaned and sterilised using ammonia for at least one night before use. All feed was formulated with no coccidiostat. Feed and water were stored at 80°C for 2 hours at least to avoid contamination. The original parent line of E. tenella, came from Hebei province of China, was cultivated from a single oocyst and the pathogenicity assay was conducted. The precocious line was obtained by repeatedly selecting early development of oocysts. Each passage was propagated by orally administering oocysts to coccidia-free chickens, and the prepatent period was detected. Oocysts were harvested from faeces a few hours later. Whenever possible, the selection pressure was applied during selection for precociousness. The mean dimensions of sporulated oocysts of the 10 th generation and parent strains were measured to determine if selection for precociousness resulted in a change in oocyst size. 100 randomly encountered oocysts were measured on the photographs pictured by microscope. Results The parent line was identified as a low virulence strain because no bird died even the inoculation dose was 1×106 sporulated oocysts. Selection for precociousness was effective in reducing the prepatent period by 11 hours through 14 generations. What's more, a significant difference in the oocysts dimensions between parent line and 10 generations of selection appeared. The mean size of sporulated oocysts of the parent line and 10 th generations of the precocious line are 22.42×17.79 and 20.14×16.76, respectively. Conclusion The results show that after pressure selection the line in the present study has possessed precocious characters. However, the selection of precocious line should be continued to obtain genetically stable strain.
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