N-3-oxo-hexanoyl-homoserine lactone, a bacterial quorum sensing molecule, plays an important role in plant salt tolerance
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- 英文论文题名：N-3-oxo-hexanoyl-homoserine lactone, a bacterial quorum sensing molecule, plays an important role in plant salt tolerance
- 论文作者：Qian Zhao ; Yao Li ; Fang Liu ; Xiangyu Cao ; Zhenhua Jia ; Shuishan Song
- 英文论文作者：Qian Zhao ; Yao Li ; Fang Liu ; Xiangyu Cao ; Zhenhua Jia ; Shuishan Song ; Biology Institute ; Hebei Academy of Sciences ; Hebei Engineering and Technology Center of Microbiological Control on Main Crop Disease
- 年：2016
- 作者机构：Biology Institute,Hebei Academy of Sciences;Hebei Engineering and Technology Center of Microbiological Control on Main Crop Disease;
- 英文论文关键词：AHL ; quorum sensing ; salt tolerance ; inter-communication ; plant
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q945.78
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：843k
- 原文格式：D
- 会议级别：全国
摘要
N-acyl-homoserine lactones(AHLs) are the quorum sensing(QS) signal molecules to coordinate the collective behavior in a population in Gram-negative bacteria. Recent evidences demonstrate their roles in plant root growth and defense responses. In this present,we show that the treatment of plant roots with N-3-oxo-hexanoyl-homoserine lactone(3OC6-HSL),one molecule of AHLs family,resulted in enhanced salt tolerance in Arabidopsis,wheat and rice. The genetic analysis revealed that the growth inhibition phenotype in the length of primary roots,the fresh weight and the height of seedlings under salt stress condition were significantly improved by 3OC6-HSL. The contents of chlorophyll were increased and the Na~+/K~+ ratios were decreased by 3OC6-HSL in Arabidopsis,wheat and rice under salt stress condition. The level of proline were raised and the expression of proline biosynthesis related genes AtP5CS,TaP5CS and the proline transport gene Ta PT were upregulated by 3OC6-HSL under salt stress condition,which suggested that the proline biosynthesis and transportation may be involved in 3OC6-HSL-mediated plant salt tolerance. q RT-PCR analysis showed that the expression of some salt-responsive genes including ABA-dependent genes RD22 and COR15 a,osmotic responsive gene ADH,ion-homeostasis related genes SOS1 and SOS2 were induced by 3OC6-HSL in Arabidopsis. But only the expression of COR15 a was upregulated with a stronger induction by 3OC6-HSL than by Na Cl under salt stress condition. 3OC6-HSL had no effect on the expression of ABA-independent gene ERD1 under salt stress condition. These results indicated that ABA-dependent salt resistant pathway might involve in AHL-induced salt tolerance. In summary,genetic,physiological and molecular evidences showed that 3OC6-HSL plays an important role in plant salt tolerance. It provides a new insight into the plant-microbe inter-communication.
N-acyl-homoserine lactones(AHLs) are the quorum sensing(QS) signal molecules to coordinate the collective behavior in a population in Gram-negative bacteria. Recent evidences demonstrate their roles in plant root growth and defense responses. In this present,we show that the treatment of plant roots with N-3-oxo-hexanoyl-homoserine lactone(3OC6-HSL),one molecule of AHLs family,resulted in enhanced salt tolerance in Arabidopsis,wheat and rice. The genetic analysis revealed that the growth inhibition phenotype in the length of primary roots,the fresh weight and the height of seedlings under salt stress condition were significantly improved by 3OC6-HSL. The contents of chlorophyll were increased and the Na~+/K~+ ratios were decreased by 3OC6-HSL in Arabidopsis,wheat and rice under salt stress condition. The level of proline were raised and the expression of proline biosynthesis related genes AtP5CS,TaP5CS and the proline transport gene Ta PT were upregulated by 3OC6-HSL under salt stress condition,which suggested that the proline biosynthesis and transportation may be involved in 3OC6-HSL-mediated plant salt tolerance. q RT-PCR analysis showed that the expression of some salt-responsive genes including ABA-dependent genes RD22 and COR15 a,osmotic responsive gene ADH,ion-homeostasis related genes SOS1 and SOS2 were induced by 3OC6-HSL in Arabidopsis. But only the expression of COR15 a was upregulated with a stronger induction by 3OC6-HSL than by Na Cl under salt stress condition. 3OC6-HSL had no effect on the expression of ABA-independent gene ERD1 under salt stress condition. These results indicated that ABA-dependent salt resistant pathway might involve in AHL-induced salt tolerance. In summary,genetic,physiological and molecular evidences showed that 3OC6-HSL plays an important role in plant salt tolerance. It provides a new insight into the plant-microbe inter-communication.
N-acyl-homoserine lactones(AHLs) are the quorum sensing(QS) signal molecules to coordinate the collective behavior in a population in Gram-negative bacteria. Recent evidences demonstrate their roles in plant root growth and defense responses. In this present,we show that the treatment of plant roots with N-3-oxo-hexanoyl-homoserine lactone(3OC6-HSL),one molecule of AHLs family,resulted in enhanced salt tolerance in Arabidopsis,wheat and rice. The genetic analysis revealed that the growth inhibition phenotype in the length of primary roots,the fresh weight and the height of seedlings under salt stress condition were significantly improved by 3OC6-HSL. The contents of chlorophyll were increased and the Na~+/K~+ ratios were decreased by 3OC6-HSL in Arabidopsis,wheat and rice under salt stress condition. The level of proline were raised and the expression of proline biosynthesis related genes AtP5CS,TaP5CS and the proline transport gene Ta PT were upregulated by 3OC6-HSL under salt stress condition,which suggested that the proline biosynthesis and transportation may be involved in 3OC6-HSL-mediated plant salt tolerance. q RT-PCR analysis showed that the expression of some salt-responsive genes including ABA-dependent genes RD22 and COR15 a,osmotic responsive gene ADH,ion-homeostasis related genes SOS1 and SOS2 were induced by 3OC6-HSL in Arabidopsis. But only the expression of COR15 a was upregulated with a stronger induction by 3OC6-HSL than by Na Cl under salt stress condition. 3OC6-HSL had no effect on the expression of ABA-independent gene ERD1 under salt stress condition. These results indicated that ABA-dependent salt resistant pathway might involve in AHL-induced salt tolerance. In summary,genetic,physiological and molecular evidences showed that 3OC6-HSL plays an important role in plant salt tolerance. It provides a new insight into the plant-microbe inter-communication.
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