Albino Leaf 1 that encodes the sole octotricopeptide repeat protein is responsible for chloroplast development in rice
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- 英文论文题名：Albino Leaf 1 that encodes the sole octotricopeptide repeat protein is responsible for chloroplast development in rice
- 论文作者：Zemin Zhang ; Jianjie Tan ; Zhenying Shi ; Qingjun Xie ; Yi Xing ; Changhong Liu ; Qiaoling Chen ; Haitao Zhu ; Jiang Wang ; Jingliu Zhang ; Guiquan Zhang
- 英文论文作者：Zemin Zhang ; Jianjie Tan ; Zhenying Shi ; Qingjun Xie ; Yi Xing ; Changhong Liu ; Qiaoling Chen ; Haitao Zhu ; Jiang Wang ; Jingliu Zhang ; Guiquan Zhang ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources ; Guangdong Provincial Key Lab of Plant Molecular Breeding ; South China Agricultural University ; Shanghai Institute of Plant Physiology and Ecology ; Chinese Academy of Sciences
- 年：2016
- 作者机构：State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangdong Provincial Key Lab of Plant Molecular Breeding,South China Agricultural University;Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences;
- 英文论文关键词：chloroplast development ; RAP ; AL1 gene ; OPR
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S511
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：852k
- 原文格式：D
- 会议级别：全国
摘要
Chloroplast,the photosynthetic organelle in plants,plays a crucial role in plant development and growth through manipulating the capacity of photosynthesis. However,the regulatory mechanism of chloroplast development still remains elusive. Here,we characterized a mutant with defective in chloroplasts in rice,termed albino leaf 1(al1),which exhibits distinct albino phenotype in leaves,eventually leading the al1 seedling to lethal. Electronic microscopy observation demonstrated that the numbers of thylakoids was reduced and the structure of thylakoids was disrupted in the al1 mutant during rice development,which eventually led to the breakdown of chloroplast. Molecular cloning revealed that AL1 encodes the sole Octotricopeptide Repeat Protein(RAP) in rice. Genetic complementation of the Arabidopsis rap mutants indicated that AL1 protein is a functional RAP. Further analysis illustrated that three transcript variants were present in the AL1 gene and the altered splices were occurred at the 3′UTR of AL1 transcript. In addition,our results also indicate that disruption of AL1 gene results in the altered expression of chloroplast associated genes. Consistently,proteomic analysis demonstrated that the abundances of photosynthesis associated proteins are significantly altered,as well as a group of metabolism associated proteins. More specifically,we found that the loss of AL1 resulted in the altered abundances of ribosomal proteins,suggesting that RAP likely also regulates the homeostasis of ribosomal proteins in rice in addition to the r RNA. Taken together,we conclude that the AL1,particularly the AL1 a and c isoform,plays an essential role in chloroplast development in rice.
Chloroplast,the photosynthetic organelle in plants,plays a crucial role in plant development and growth through manipulating the capacity of photosynthesis. However,the regulatory mechanism of chloroplast development still remains elusive. Here,we characterized a mutant with defective in chloroplasts in rice,termed albino leaf 1(al1),which exhibits distinct albino phenotype in leaves,eventually leading the al1 seedling to lethal. Electronic microscopy observation demonstrated that the numbers of thylakoids was reduced and the structure of thylakoids was disrupted in the al1 mutant during rice development,which eventually led to the breakdown of chloroplast. Molecular cloning revealed that AL1 encodes the sole Octotricopeptide Repeat Protein(RAP) in rice. Genetic complementation of the Arabidopsis rap mutants indicated that AL1 protein is a functional RAP. Further analysis illustrated that three transcript variants were present in the AL1 gene and the altered splices were occurred at the 3′UTR of AL1 transcript. In addition,our results also indicate that disruption of AL1 gene results in the altered expression of chloroplast associated genes. Consistently,proteomic analysis demonstrated that the abundances of photosynthesis associated proteins are significantly altered,as well as a group of metabolism associated proteins. More specifically,we found that the loss of AL1 resulted in the altered abundances of ribosomal proteins,suggesting that RAP likely also regulates the homeostasis of ribosomal proteins in rice in addition to the r RNA. Taken together,we conclude that the AL1,particularly the AL1 a and c isoform,plays an essential role in chloroplast development in rice.
Chloroplast,the photosynthetic organelle in plants,plays a crucial role in plant development and growth through manipulating the capacity of photosynthesis. However,the regulatory mechanism of chloroplast development still remains elusive. Here,we characterized a mutant with defective in chloroplasts in rice,termed albino leaf 1(al1),which exhibits distinct albino phenotype in leaves,eventually leading the al1 seedling to lethal. Electronic microscopy observation demonstrated that the numbers of thylakoids was reduced and the structure of thylakoids was disrupted in the al1 mutant during rice development,which eventually led to the breakdown of chloroplast. Molecular cloning revealed that AL1 encodes the sole Octotricopeptide Repeat Protein(RAP) in rice. Genetic complementation of the Arabidopsis rap mutants indicated that AL1 protein is a functional RAP. Further analysis illustrated that three transcript variants were present in the AL1 gene and the altered splices were occurred at the 3′UTR of AL1 transcript. In addition,our results also indicate that disruption of AL1 gene results in the altered expression of chloroplast associated genes. Consistently,proteomic analysis demonstrated that the abundances of photosynthesis associated proteins are significantly altered,as well as a group of metabolism associated proteins. More specifically,we found that the loss of AL1 resulted in the altered abundances of ribosomal proteins,suggesting that RAP likely also regulates the homeostasis of ribosomal proteins in rice in addition to the r RNA. Taken together,we conclude that the AL1,particularly the AL1 a and c isoform,plays an essential role in chloroplast development in rice.
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