Epithelial and glandular evidence of exosomes and secretions in the oviduct of Chinese soft-shelled turtle,Pelodiscus sinensis
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- 英文论文题名：Epithelial and glandular evidence of exosomes and secretions in the oviduct of Chinese soft-shelled turtle,Pelodiscus sinensis
- 论文作者：Muhammad Yasir Waqas ; Hu Lisi ; Ping Yang ; Shakeeb Ullah ; Linli Zhang ; Qian Zhang ; Quanfu Li ; Nisar Ahmad ; Wei Chen ; Qiusheng Chen
- 英文论文作者：Muhammad Yasir Waqas ; Hu Lisi ; Ping Yang ; Shakeeb Ullah ; Linli Zhang ; Qian Zhang ; Quanfu Li ; Nisar Ahmad ; Wei Chen ; Qiusheng Chen ; College of Veterinary Medicine ; Nanjing Agricultural University
- 年：2016
- 作者机构：College of Veterinary Medicine,Nanjing Agricultural University;
- 英文论文关键词：exosomes and secretions ; oviduct secretions ; Pelodiscus sinensis
- 会议召开时间：2016-08-20
- 会议录名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会论文集
- 语种：英文
- 分类号：S917.4
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会
- 会议地点：中国河南郑州
- 主办单位：中国畜牧兽医学会动物解剖及组织胚胎学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：58k
- 原文格式：O
- 会议级别：全国
摘要
The Oviduct is a site of egg production,fertilization and sperm storage while,its secretions have broadly attributes towards different physiology functions.We examined the ultrastructure of oviduct epithelium and glands in relation with secretions particularly with exosomes origin in Chinese soft shelled turtle Pelodiscus sinensis using,immunohistochemistry and transmission electron microscopy.The epithelial ciliated and gland cells were involved in the release of exosomes and secretions into the lumen throughout the year;the exosomes were either released directly from epithelium or in relation with multivesicular body(MVB).The average size of the particles varies between(50-130nm).These exosomes were also widely distributed to the epithelial ciliated cells and in peri-cytoplasm of glands lumen.Intracellular MVB was characterized with the different size membrane bounded exosomes.Exosomes were also found in close contact with the cilia and sperm membrane in the lumen which is suggestive of their fusogenic properties.Strong to moderate positive localization of exosomes was noted in ciliated and gland cells during January,September and December as it is the time of sperm storage in this turtle while,it showed moderate to weak level of localization during breeding season(May).This is first study about the identification of the exosomes in the reptiles.Epithelial and glandular intracellular and extracellular exosomes,intracellular MVB,secretions and secretory vesicles while,the release of exosomes throughout the year gives this turtle specie a unique secretory morphology and a potential model for investigating the secretory nature of the oviduct.
The Oviduct is a site of egg production,fertilization and sperm storage while,its secretions have broadly attributes towards different physiology functions.We examined the ultrastructure of oviduct epithelium and glands in relation with secretions particularly with exosomes origin in Chinese soft shelled turtle Pelodiscus sinensis using,immunohistochemistry and transmission electron microscopy.The epithelial ciliated and gland cells were involved in the release of exosomes and secretions into the lumen throughout the year;the exosomes were either released directly from epithelium or in relation with multivesicular body(MVB).The average size of the particles varies between(50-130nm).These exosomes were also widely distributed to the epithelial ciliated cells and in peri-cytoplasm of glands lumen.Intracellular MVB was characterized with the different size membrane bounded exosomes.Exosomes were also found in close contact with the cilia and sperm membrane in the lumen which is suggestive of their fusogenic properties.Strong to moderate positive localization of exosomes was noted in ciliated and gland cells during January,September and December as it is the time of sperm storage in this turtle while,it showed moderate to weak level of localization during breeding season(May).This is first study about the identification of the exosomes in the reptiles.Epithelial and glandular intracellular and extracellular exosomes,intracellular MVB,secretions and secretory vesicles while,the release of exosomes throughout the year gives this turtle specie a unique secretory morphology and a potential model for investigating the secretory nature of the oviduct.
The Oviduct is a site of egg production,fertilization and sperm storage while,its secretions have broadly attributes towards different physiology functions.We examined the ultrastructure of oviduct epithelium and glands in relation with secretions particularly with exosomes origin in Chinese soft shelled turtle Pelodiscus sinensis using,immunohistochemistry and transmission electron microscopy.The epithelial ciliated and gland cells were involved in the release of exosomes and secretions into the lumen throughout the year;the exosomes were either released directly from epithelium or in relation with multivesicular body(MVB).The average size of the particles varies between(50-130nm).These exosomes were also widely distributed to the epithelial ciliated cells and in peri-cytoplasm of glands lumen.Intracellular MVB was characterized with the different size membrane bounded exosomes.Exosomes were also found in close contact with the cilia and sperm membrane in the lumen which is suggestive of their fusogenic properties.Strong to moderate positive localization of exosomes was noted in ciliated and gland cells during January,September and December as it is the time of sperm storage in this turtle while,it showed moderate to weak level of localization during breeding season(May).This is first study about the identification of the exosomes in the reptiles.Epithelial and glandular intracellular and extracellular exosomes,intracellular MVB,secretions and secretory vesicles while,the release of exosomes throughout the year gives this turtle specie a unique secretory morphology and a potential model for investigating the secretory nature of the oviduct.
引文