Mechanism research of flower pigment mutation induced by carbon ions in geranium
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- 英文论文题名：Mechanism research of flower pigment mutation induced by carbon ions in geranium
- 论文作者：Lixia Yu ; Yan Du ; Libin Zhou
- 英文论文作者：Lixia Yu ; Yan Du ; Libin Zhou ; Biophysics Group ; Institute of Modern Physics ; Chinese Academy of Sciences
- 年：2016
- 作者机构：Biophysics Group,Institute of Modern Physics,Chinese Academy of Sciences;
- 英文论文关键词：carbon ions irradiation ; flower color mutant ; depigmentation
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：841k
- 原文格式：D
- 会议级别：全国
摘要
Heavy ion mutation technology as a unique and efficient mutagen has been widely used in germplasm innovation and plant breeding. Flower color as the important qualitative character and economic indicator for ornamental plants become the primary target for ornamental breeding. Here we report one flower color mutant obtained using carbon ion irradiation and attempt to elucidate underlying mutation mechanism. The petal color of the mutant changed from orange-red to light pink,and colors of peduncle,pistil and stamen also displayed significant differences with wild type. Anatomical structure observations showed that the salmon pigments fully filled with the epidermal cells of wild type,while only a small number of pale pink pigments in mutant. HPLC results indicated that the main pigments determined the flower color of wild type were pelargonidin,cyanidin and delphinidin. On the contrary,the unique pigment detected in mutant was cyanidin. The core reason of depigmentation in mutant was the absence of pelargonidin and delphinidin. Quantitative real-time PCR analysis suggested that the significantly down-regulated transcriptional level of early genes CHS,CHI and suppressed expression of gene ANS blocked the biosynthesis of anthocyanin,and that was the crucial reason for the deficiency of anthocyanin which lead to the depigmentation of mutant. These results explained the mechanism of coloration change in mutant induced by carbon ions and provided the research foundation for the follow-up design and color improving in geranium.
Heavy ion mutation technology as a unique and efficient mutagen has been widely used in germplasm innovation and plant breeding. Flower color as the important qualitative character and economic indicator for ornamental plants become the primary target for ornamental breeding. Here we report one flower color mutant obtained using carbon ion irradiation and attempt to elucidate underlying mutation mechanism. The petal color of the mutant changed from orange-red to light pink,and colors of peduncle,pistil and stamen also displayed significant differences with wild type. Anatomical structure observations showed that the salmon pigments fully filled with the epidermal cells of wild type,while only a small number of pale pink pigments in mutant. HPLC results indicated that the main pigments determined the flower color of wild type were pelargonidin,cyanidin and delphinidin. On the contrary,the unique pigment detected in mutant was cyanidin. The core reason of depigmentation in mutant was the absence of pelargonidin and delphinidin. Quantitative real-time PCR analysis suggested that the significantly down-regulated transcriptional level of early genes CHS,CHI and suppressed expression of gene ANS blocked the biosynthesis of anthocyanin,and that was the crucial reason for the deficiency of anthocyanin which lead to the depigmentation of mutant. These results explained the mechanism of coloration change in mutant induced by carbon ions and provided the research foundation for the follow-up design and color improving in geranium.
Heavy ion mutation technology as a unique and efficient mutagen has been widely used in germplasm innovation and plant breeding. Flower color as the important qualitative character and economic indicator for ornamental plants become the primary target for ornamental breeding. Here we report one flower color mutant obtained using carbon ion irradiation and attempt to elucidate underlying mutation mechanism. The petal color of the mutant changed from orange-red to light pink,and colors of peduncle,pistil and stamen also displayed significant differences with wild type. Anatomical structure observations showed that the salmon pigments fully filled with the epidermal cells of wild type,while only a small number of pale pink pigments in mutant. HPLC results indicated that the main pigments determined the flower color of wild type were pelargonidin,cyanidin and delphinidin. On the contrary,the unique pigment detected in mutant was cyanidin. The core reason of depigmentation in mutant was the absence of pelargonidin and delphinidin. Quantitative real-time PCR analysis suggested that the significantly down-regulated transcriptional level of early genes CHS,CHI and suppressed expression of gene ANS blocked the biosynthesis of anthocyanin,and that was the crucial reason for the deficiency of anthocyanin which lead to the depigmentation of mutant. These results explained the mechanism of coloration change in mutant induced by carbon ions and provided the research foundation for the follow-up design and color improving in geranium.
引文