Selection and Validation of Reference Genes for Gene Expression Analysis in Vigna angularis Using Quantitative Real-Time RT-PCR
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- 英文论文题名：Selection and Validation of Reference Genes for Gene Expression Analysis in Vigna angularis Using Quantitative Real-Time RT-PCR
- 论文作者：SHEN Yong-qiang ; KE Xi-wang ; YIN Li-hua ; HAN Dong ; ZUO Yu-hu
- 英文论文作者：SHEN Yong-qiang ; KE Xi-wang ; YIN Li-hua ; HAN Dong ; ZUO Yu-hu ; Heilongjiang Bayi Agricultural University
- 年：2016
- 作者机构：Heilongjiang Bayi Agricultural University;
- 英文论文关键词：Adzuki bean ; Quantitative real time PCR ; Reference genes
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S521
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：41k
- 原文格式：O
- 会议级别：全国
摘要
Adzuki bean(Vigna angularis) is one of the most important legume crops in Asia due to its nutritious protein and starch contents,as well as its useful potential for cropping structure adjustment in China.In spite of its economic importance,gene expression analysis system for gene function verification of adzuki bean is still absent.Therefore,reference genes for gene expression analysis based on the quantitative real time PCR(qRT-PCR) were screened in current study.A total of nine general housekeeping genes,including ACT,Fbox,ZMPP,GAPDH,EF,PP2 A,UBC,UBN and PTB were evaluated for their expression stability by qRT-PCR in four adzuki bean cultivars,three different tissues,four abiotic stress and a biotic stress.The best group of candidates as reference genes were as follows:PP2A and PTB for different cultivars;EF,PP2 A and UBN for different tissues;ACT,UBC and UBN for biotic stress;ACT and ZMPP for waterlogging stress;Fbox,UBC and UBN for salinity-alkalinity stress;and Fbox,ACT and PTB for drought stress.Our results will provide a more accurate and reliable normalization of qRT-PCR data in adzuki bean.
Adzuki bean(Vigna angularis) is one of the most important legume crops in Asia due to its nutritious protein and starch contents,as well as its useful potential for cropping structure adjustment in China.In spite of its economic importance,gene expression analysis system for gene function verification of adzuki bean is still absent.Therefore,reference genes for gene expression analysis based on the quantitative real time PCR(qRT-PCR) were screened in current study.A total of nine general housekeeping genes,including ACT,Fbox,ZMPP,GAPDH,EF,PP2 A,UBC,UBN and PTB were evaluated for their expression stability by qRT-PCR in four adzuki bean cultivars,three different tissues,four abiotic stress and a biotic stress.The best group of candidates as reference genes were as follows:PP2A and PTB for different cultivars;EF,PP2 A and UBN for different tissues;ACT,UBC and UBN for biotic stress;ACT and ZMPP for waterlogging stress;Fbox,UBC and UBN for salinity-alkalinity stress;and Fbox,ACT and PTB for drought stress.Our results will provide a more accurate and reliable normalization of qRT-PCR data in adzuki bean.
Adzuki bean(Vigna angularis) is one of the most important legume crops in Asia due to its nutritious protein and starch contents,as well as its useful potential for cropping structure adjustment in China.In spite of its economic importance,gene expression analysis system for gene function verification of adzuki bean is still absent.Therefore,reference genes for gene expression analysis based on the quantitative real time PCR(qRT-PCR) were screened in current study.A total of nine general housekeeping genes,including ACT,Fbox,ZMPP,GAPDH,EF,PP2 A,UBC,UBN and PTB were evaluated for their expression stability by qRT-PCR in four adzuki bean cultivars,three different tissues,four abiotic stress and a biotic stress.The best group of candidates as reference genes were as follows:PP2A and PTB for different cultivars;EF,PP2 A and UBN for different tissues;ACT,UBC and UBN for biotic stress;ACT and ZMPP for waterlogging stress;Fbox,UBC and UBN for salinity-alkalinity stress;and Fbox,ACT and PTB for drought stress.Our results will provide a more accurate and reliable normalization of qRT-PCR data in adzuki bean.
引文