A simpler,multiple loci and more precise genome editing technology system for crop
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- 英文论文题名：A simpler,multiple loci and more precise genome editing technology system for crop
- 论文作者：Chengwei Zhang ; Wen Xu ; Ying Wu ; Xiang Zhang ; Jinxiao Yang
- 英文论文作者：Chengwei Zhang ; Wen Xu ; Ying Wu ; Xiang Zhang ; Jinxiao Yang ; Genome Editing Lab ; Da Bei Nong Group
- 年：2016
- 作者机构：Genome Editing Lab,Da Bei Nong Group;
- 英文论文关键词：CRISPR/Cas9 ; multi-gene editing ; nucleotide substitution
- 会议召开时间：2016-09-01
- 会议录名称：全国农业生物化学与分子生物学第十五届学术研讨会会议文集
- 英文会议录名称：The Special Edition of the 15th National Symposium of Agricultural Biochemistry and Molecular Biology
- 语种：英文
- 分类号：S188
- 学会代码：IGSS
- 会议名称：全国农业生物化学与分子生物学第十五届学术研讨会
- 会议地点：中国甘肃兰州
- 主办单位：中国生物化学与分子生物学会农业分会
- 学会名称：中国生物化学与分子生物学会
- 页数：1
- 文件大小：29k
- 原文格式：O
- 会议级别：全国
摘要
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications and crop improvement.Not only for single gene kocck out,but also for multiple targeted mutagenesis and functional nucleotide substitution technology will be mostly employed for elite allele creation,especially for the numberous quantitative trait locuses.To make the multiplexity of CRISPR/Cas9,We tested different type Ⅱ CRISPR/Cas systems and demonstrate that they worked very well in rice.In the best system,we realized 6 loci mutation one time with above 30%efficiency.To develop functional nucleotide substitution technology,we employed CRISPR/Cas9,positive-negtive selection to realized two fuction nucleotide subtitution in one important trait gene with a not very high efficiency.When we used homolog recombination improving effect,the efficiency can be improved above 2%,which should be engouth for some important genes testing in crop improvement.Combine these two methods for multi-trait impromovment or special quantitative trait locus editing will be carried out in nect step.
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications and crop improvement.Not only for single gene kocck out,but also for multiple targeted mutagenesis and functional nucleotide substitution technology will be mostly employed for elite allele creation,especially for the numberous quantitative trait locuses.To make the multiplexity of CRISPR/Cas9,We tested different type Ⅱ CRISPR/Cas systems and demonstrate that they worked very well in rice.In the best system,we realized 6 loci mutation one time with above 30%efficiency.To develop functional nucleotide substitution technology,we employed CRISPR/Cas9,positive-negtive selection to realized two fuction nucleotide subtitution in one important trait gene with a not very high efficiency.When we used homolog recombination improving effect,the efficiency can be improved above 2%,which should be engouth for some important genes testing in crop improvement.Combine these two methods for multi-trait impromovment or special quantitative trait locus editing will be carried out in nect step.
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications and crop improvement.Not only for single gene kocck out,but also for multiple targeted mutagenesis and functional nucleotide substitution technology will be mostly employed for elite allele creation,especially for the numberous quantitative trait locuses.To make the multiplexity of CRISPR/Cas9,We tested different type Ⅱ CRISPR/Cas systems and demonstrate that they worked very well in rice.In the best system,we realized 6 loci mutation one time with above 30%efficiency.To develop functional nucleotide substitution technology,we employed CRISPR/Cas9,positive-negtive selection to realized two fuction nucleotide subtitution in one important trait gene with a not very high efficiency.When we used homolog recombination improving effect,the efficiency can be improved above 2%,which should be engouth for some important genes testing in crop improvement.Combine these two methods for multi-trait impromovment or special quantitative trait locus editing will be carried out in nect step.
引文