Genomic characteristics and QTL identifications of chromosomal single-segment substitution lines of O. meridionalis in the genetic background of O. sativa
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- 英文论文题名：Genomic characteristics and QTL identifications of chromosomal single-segment substitution lines of O. meridionalis in the genetic background of O. sativa
- 论文作者：Xiaoping Pan ; Rouxian Wu ; Liping Peng ; Kang Sun ; Haitao Zhu ; Ruizhen Zeng ; Shaokui Wang ; Guiquan Zhang ; Xuelin Fu
- 英文论文作者：Xiaoping Pan ; Rouxian Wu ; Liping Peng ; Kang Sun ; Haitao Zhu ; Ruizhen Zeng ; Shaokui Wang ; Guiquan Zhang ; Xuelin Fu ; Key Laboratory of Plant Molecular Breeding ; Guangdong Province/College of Agriculture ; South China Agricultural University
- 年：2016
- 作者机构：Key Laboratory of Plant Molecular Breeding,Guangdong Province/College of Agriculture,South China Agricultural University;
- 英文论文关键词：O. meridionalis ; chromosomal single-segment substitution lines(SSSLs) ; QTL identification ; substitution mapping
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S511
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：842k
- 原文格式：D
- 会议级别：全国
摘要
O. meridionalis indigenous to Australia is an AA genome wild species in Oryza,it has been considered an important germplasm resource for improvement of cultivated rice. In this study,a total of 99 chromosomal single-segment substitution lines(SSSLs) were developed by using O. meridionalis as a donor and an elite indica cultivar,Huajingxian 74 as recipient,through successive backcrossing and SSR marker-based genotyping. The 99 substituted segments were distributed on 12 chromosomes with the estimated total lengths of 1580.16 cM and 15.11 c M mean length of every segment. The 837.94 c M coverage length of substituted segments covered 54.98 percent of rice genome. Fourteen quantitative traits were evaluated in SSSLs for two seasons,including plant vegetative growth traits,i.e.,heading date,plant height,flag leaf length,flag leaf width; Grain yield related traits,i.e.,panicle numbers per plant,panicle length,spikelet numbers per panicle,grain density,number of primary branches per panicle,seed-setting rate,100-grain weight,grain length,grain width,and ratio of grain length to width. A total of 161 putative QTLs detected for thirteen traits except panicle numbers per plant. Among them,32 QTLs related to nine traits were identified both in the late season of 2014 and the early season of 2015,in which 10 QTLs had the positive additive effects. The additive effect of the QTLs ranged from 0.10 to 33.55,and the contribution of the additive effect to traits were from 0.06-20.92 percent. The present study demonstrated that the SSSLs offer both opportunities and a good germplasm platform for identification and transformation beneficial genes of O. meridionalis,as well as a precious long-time in-vivo conservation reservoir for O. meridionalis.
O. meridionalis indigenous to Australia is an AA genome wild species in Oryza,it has been considered an important germplasm resource for improvement of cultivated rice. In this study,a total of 99 chromosomal single-segment substitution lines(SSSLs) were developed by using O. meridionalis as a donor and an elite indica cultivar,Huajingxian 74 as recipient,through successive backcrossing and SSR marker-based genotyping. The 99 substituted segments were distributed on 12 chromosomes with the estimated total lengths of 1580.16 cM and 15.11 c M mean length of every segment. The 837.94 c M coverage length of substituted segments covered 54.98 percent of rice genome. Fourteen quantitative traits were evaluated in SSSLs for two seasons,including plant vegetative growth traits,i.e.,heading date,plant height,flag leaf length,flag leaf width; Grain yield related traits,i.e.,panicle numbers per plant,panicle length,spikelet numbers per panicle,grain density,number of primary branches per panicle,seed-setting rate,100-grain weight,grain length,grain width,and ratio of grain length to width. A total of 161 putative QTLs detected for thirteen traits except panicle numbers per plant. Among them,32 QTLs related to nine traits were identified both in the late season of 2014 and the early season of 2015,in which 10 QTLs had the positive additive effects. The additive effect of the QTLs ranged from 0.10 to 33.55,and the contribution of the additive effect to traits were from 0.06-20.92 percent. The present study demonstrated that the SSSLs offer both opportunities and a good germplasm platform for identification and transformation beneficial genes of O. meridionalis,as well as a precious long-time in-vivo conservation reservoir for O. meridionalis.
O. meridionalis indigenous to Australia is an AA genome wild species in Oryza,it has been considered an important germplasm resource for improvement of cultivated rice. In this study,a total of 99 chromosomal single-segment substitution lines(SSSLs) were developed by using O. meridionalis as a donor and an elite indica cultivar,Huajingxian 74 as recipient,through successive backcrossing and SSR marker-based genotyping. The 99 substituted segments were distributed on 12 chromosomes with the estimated total lengths of 1580.16 cM and 15.11 c M mean length of every segment. The 837.94 c M coverage length of substituted segments covered 54.98 percent of rice genome. Fourteen quantitative traits were evaluated in SSSLs for two seasons,including plant vegetative growth traits,i.e.,heading date,plant height,flag leaf length,flag leaf width; Grain yield related traits,i.e.,panicle numbers per plant,panicle length,spikelet numbers per panicle,grain density,number of primary branches per panicle,seed-setting rate,100-grain weight,grain length,grain width,and ratio of grain length to width. A total of 161 putative QTLs detected for thirteen traits except panicle numbers per plant. Among them,32 QTLs related to nine traits were identified both in the late season of 2014 and the early season of 2015,in which 10 QTLs had the positive additive effects. The additive effect of the QTLs ranged from 0.10 to 33.55,and the contribution of the additive effect to traits were from 0.06-20.92 percent. The present study demonstrated that the SSSLs offer both opportunities and a good germplasm platform for identification and transformation beneficial genes of O. meridionalis,as well as a precious long-time in-vivo conservation reservoir for O. meridionalis.
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