Screening and Analysis of Effector Proteins from Puccinia triticina by Transcriptome Sequencing
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- 英文论文题名：Screening and Analysis of Effector Proteins from Puccinia triticina by Transcriptome Sequencing
- 论文作者：LI Jian-yuan ; WANG Zhi ; ZHANG Yu-mei ; YANG Wen-xiang ; LIU Da-qun
- 英文论文作者：LI Jian-yuan ; WANG Zhi ; ZHANG Yu-mei ; YANG Wen-xiang ; LIU Da-qun ; Department of Plant Pathology ; Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas
- 年：2016
- 作者机构：Department of Plant Pathology,Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas;
- 英文论文关键词：Puccinia triticina ; Effector proteins ; Transcriptome sequencing
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S435.121.43
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：49k
- 原文格式：O
- 会议级别：全国
摘要
Leaf rust caused by the fungus Puccinia triticina(Pt) is a major constraint to wheat production worldwide.The molecular events that underlie Pt pathogenicity are largely unknown.Like all rusts,Pt forms a specialized infection structure called haustoria to take nutrients and water from host plant tissue,and to secrete pathogenicity factors called effector proteins.We extracted the total RNA of urediniospores,germinated urediniospores and infected wheat leaves harvest at 6d from three different virulence strains of Pt and used Illumina Hiseq sequencing platforms to assemble the transcriptomes and then we got 80392 Unigenes.Candidate effectors were screened through Signal P 4.1,Target P 1.1,TMHMM 2.0 and Effector P,and 325 genes which constitute candidate effectors were identified.About two thirds of these were up-regulated in the harvest leaves compared to urediniospores and germinated spores.42 of the genes were predicted to code for proteins of known function like a variety of metabolism processes,nutrient transport,and biosynthesis by using a BLAST2 GO platform and manual annotation.Pfam was used to predict the frameworks and 61 proteins were predicted with Pfam notes.The remaining 264 secreted proteins were screened and predicted the de novo structure by using MEME software.The results showed that 3 conserved domains were found in 11 effector proteins.RT-PCR analysis confirmed the expression patterns of 52 effector candidates and four genes showed a special increase in infected leaves which may be associated with the formation of urediniospores.This analysis of candidate effectors is an initial step towards functional research and established the foundation for clarify the pathogenic mechanism of leaf rust pathogens.
Leaf rust caused by the fungus Puccinia triticina(Pt) is a major constraint to wheat production worldwide.The molecular events that underlie Pt pathogenicity are largely unknown.Like all rusts,Pt forms a specialized infection structure called haustoria to take nutrients and water from host plant tissue,and to secrete pathogenicity factors called effector proteins.We extracted the total RNA of urediniospores,germinated urediniospores and infected wheat leaves harvest at 6d from three different virulence strains of Pt and used Illumina Hiseq sequencing platforms to assemble the transcriptomes and then we got 80392 Unigenes.Candidate effectors were screened through Signal P 4.1,Target P 1.1,TMHMM 2.0 and Effector P,and 325 genes which constitute candidate effectors were identified.About two thirds of these were up-regulated in the harvest leaves compared to urediniospores and germinated spores.42 of the genes were predicted to code for proteins of known function like a variety of metabolism processes,nutrient transport,and biosynthesis by using a BLAST2 GO platform and manual annotation.Pfam was used to predict the frameworks and 61 proteins were predicted with Pfam notes.The remaining 264 secreted proteins were screened and predicted the de novo structure by using MEME software.The results showed that 3 conserved domains were found in 11 effector proteins.RT-PCR analysis confirmed the expression patterns of 52 effector candidates and four genes showed a special increase in infected leaves which may be associated with the formation of urediniospores.This analysis of candidate effectors is an initial step towards functional research and established the foundation for clarify the pathogenic mechanism of leaf rust pathogens.
Leaf rust caused by the fungus Puccinia triticina(Pt) is a major constraint to wheat production worldwide.The molecular events that underlie Pt pathogenicity are largely unknown.Like all rusts,Pt forms a specialized infection structure called haustoria to take nutrients and water from host plant tissue,and to secrete pathogenicity factors called effector proteins.We extracted the total RNA of urediniospores,germinated urediniospores and infected wheat leaves harvest at 6d from three different virulence strains of Pt and used Illumina Hiseq sequencing platforms to assemble the transcriptomes and then we got 80392 Unigenes.Candidate effectors were screened through Signal P 4.1,Target P 1.1,TMHMM 2.0 and Effector P,and 325 genes which constitute candidate effectors were identified.About two thirds of these were up-regulated in the harvest leaves compared to urediniospores and germinated spores.42 of the genes were predicted to code for proteins of known function like a variety of metabolism processes,nutrient transport,and biosynthesis by using a BLAST2 GO platform and manual annotation.Pfam was used to predict the frameworks and 61 proteins were predicted with Pfam notes.The remaining 264 secreted proteins were screened and predicted the de novo structure by using MEME software.The results showed that 3 conserved domains were found in 11 effector proteins.RT-PCR analysis confirmed the expression patterns of 52 effector candidates and four genes showed a special increase in infected leaves which may be associated with the formation of urediniospores.This analysis of candidate effectors is an initial step towards functional research and established the foundation for clarify the pathogenic mechanism of leaf rust pathogens.
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