Cloning,Expression and Function Analysis of TaSGT1 in TcLr19
详细信息 查看官网全文
- 英文论文题名：Cloning,Expression and Function Analysis of TaSGT1 in TcLr19
- 论文作者：YUAN Ying ; HU Ya-ya ; LI Jan-yuan ; YANG Wen-xiang ; LIU Da-qun
- 英文论文作者：YUAN Ying ; HU Ya-ya ; LI Jan-yuan ; YANG Wen-xiang ; LIU Da-qun ; Department of Plant Pathology ; Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas ; Institute of Cereal and Oil crops ; Hebei academy of Agriculture and Forestry Sciences
- 年：2016
- 作者机构：Department of Plant Pathology,Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas;Institute of Cereal and Oil crops,Hebei academy of Agriculture and Forestry Sciences;
- 英文论文关键词：Wheat leaf rust ; TaSGT1 ; Rapid amplification cDNA ends(RACE) ; Virus induced gene silencing(VIGS) ; Subcellular localization ; Real-time quantitative PCR
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S435.121.43
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：51k
- 原文格式：O
- 会议级别：全国
摘要
Wheat leaf rust,caused by Puccinia triticina is an airborne fungal disease in wheat worldwide,which can cause significant yield losses to the wheat production in the world.Using resistant cultivars is the most economical and environmentally sound method to control the disease.A leaf rust resistance gene Lr19 could provides resistance to all races of P.triticina currently found in China,and has shown high potential in application at home and abroad.The objectives of this work were to clone a TaSGT1 gene from the TcLr19 wheat line and understanding its function in against the wheat leaf rust.A spliced sequence with the length of 1 690 bp was obtained by rapid amplification cDNA ends(RACE),and the full length cDNA of the TaSGT1 gene isolated from TcLr19 was 1 372 bp.The TaSGT1 protein,containing TPR,VR1,CS,VR2 and SGS domains,was predicted by using the SMART software.TaSGT1 gene was located on plasma membrane and nucleus by subcellular localization.Silencing of TaSGT1 in the leaf rust-resistant plants by virus induced gene silencing(VIGS) resulted in increased susceptibility to P.triticina.Further histological observation found that mycelium of P.triticina grew with small area hypersensitive cell death at 120 hpi in plants with silenced gene TaSGT1.Temporal and spatial expression profile of TaSGT1 was detected by real-time quantification PCR.The results showed that TaSGT1 transcript was up-regulated inoculated with leaf rust and TaSGT1 could be induced by salicylic acid(SA) but not induced by abscisic acid(ABA).All those results indicated that TaSGT1 is an important role involved in Lr19 resistance expression and SA induced resistance signal channel.
Wheat leaf rust,caused by Puccinia triticina is an airborne fungal disease in wheat worldwide,which can cause significant yield losses to the wheat production in the world.Using resistant cultivars is the most economical and environmentally sound method to control the disease.A leaf rust resistance gene Lr19 could provides resistance to all races of P.triticina currently found in China,and has shown high potential in application at home and abroad.The objectives of this work were to clone a TaSGT1 gene from the TcLr19 wheat line and understanding its function in against the wheat leaf rust.A spliced sequence with the length of 1 690 bp was obtained by rapid amplification cDNA ends(RACE),and the full length cDNA of the TaSGT1 gene isolated from TcLr19 was 1 372 bp.The TaSGT1 protein,containing TPR,VR1,CS,VR2 and SGS domains,was predicted by using the SMART software.TaSGT1 gene was located on plasma membrane and nucleus by subcellular localization.Silencing of TaSGT1 in the leaf rust-resistant plants by virus induced gene silencing(VIGS) resulted in increased susceptibility to P.triticina.Further histological observation found that mycelium of P.triticina grew with small area hypersensitive cell death at 120 hpi in plants with silenced gene TaSGT1.Temporal and spatial expression profile of TaSGT1 was detected by real-time quantification PCR.The results showed that TaSGT1 transcript was up-regulated inoculated with leaf rust and TaSGT1 could be induced by salicylic acid(SA) but not induced by abscisic acid(ABA).All those results indicated that TaSGT1 is an important role involved in Lr19 resistance expression and SA induced resistance signal channel.
Wheat leaf rust,caused by Puccinia triticina is an airborne fungal disease in wheat worldwide,which can cause significant yield losses to the wheat production in the world.Using resistant cultivars is the most economical and environmentally sound method to control the disease.A leaf rust resistance gene Lr19 could provides resistance to all races of P.triticina currently found in China,and has shown high potential in application at home and abroad.The objectives of this work were to clone a TaSGT1 gene from the TcLr19 wheat line and understanding its function in against the wheat leaf rust.A spliced sequence with the length of 1 690 bp was obtained by rapid amplification cDNA ends(RACE),and the full length cDNA of the TaSGT1 gene isolated from TcLr19 was 1 372 bp.The TaSGT1 protein,containing TPR,VR1,CS,VR2 and SGS domains,was predicted by using the SMART software.TaSGT1 gene was located on plasma membrane and nucleus by subcellular localization.Silencing of TaSGT1 in the leaf rust-resistant plants by virus induced gene silencing(VIGS) resulted in increased susceptibility to P.triticina.Further histological observation found that mycelium of P.triticina grew with small area hypersensitive cell death at 120 hpi in plants with silenced gene TaSGT1.Temporal and spatial expression profile of TaSGT1 was detected by real-time quantification PCR.The results showed that TaSGT1 transcript was up-regulated inoculated with leaf rust and TaSGT1 could be induced by salicylic acid(SA) but not induced by abscisic acid(ABA).All those results indicated that TaSGT1 is an important role involved in Lr19 resistance expression and SA induced resistance signal channel.
引文