A conserved Puccinia striiformis effector interacts with wheat NPR1 and reduces induction of pathogenesis-relatedgenes in response to pathogens
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- 英文论文题名：A conserved Puccinia striiformis effector interacts with wheat NPR1 and reduces induction of pathogenesis-relatedgenes in response to pathogens
- 论文作者：WANG Xiao-dong ; YANG Bao-ju ; LI Kun ; KANG Zhen-sheng ; Dario Cantu ; Jorge Dubcovsky
- 英文论文作者：WANG Xiao-dong ; YANG Bao-ju ; LI Kun ; KANG Zhen-sheng ; Dario Cantu ; Jorge Dubcovsky ; Department of Plant Science ; University of California ; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection ; Northwest Agriculture and Forestry University ; Department of Plant Pathology ; Agriculture University of Hebei ; Department of Viticulture and Enology ; University of California ; Davis ; CA 95616 ; USA.5.Howard Hughes Medical Institute(HHMI) ; Chevy Chase
- 年：2016
- 作者机构：Department of Plant Science,University of California;State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection,Northwest Agriculture and Forestry University;Department of Plant Pathology,Agriculture University of Hebei;Department of Viticulture and Enology,University of California,Davis,CA 95616,USA.5.Howard Hughes Medical Institute(HHMI),Chevy Chase;
- 英文论文关键词：Wheat ; Stripe rust ; NPR1 ; Pathogen effector ; Yeast two-hybrid ; Bimolecular fluorescence complementation ; Transgenic barley
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S435.121.4
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：49k
- 原文格式：O
- 会议级别：全国
摘要
In Arabidopsis,NPR1 is a key transcriptional co-regulator of systemic acquired resistance.Upon pathogen challenge,NPR1 translocates from the cytoplasm to the nucleus,where it interacts with TGA-bZIP transcription factors to activate the expression of several pathogenesis-related genes.In a screen of a yeast two-hybrid library from wheat leaves infected with Pucciniastriiformisf.sp.tritici,we identified a conserved rust effector that interacts with wheat NPR1 and named it Puccinia NPR1 interactor(PNPi).PNPi interacts with the NPR1/NIM1-like domain of NPR1 via its C-terminal domain.Using bimolecular fluorescence complementation assays,we detected the interaction between PNPi and wheat NPR1 in the nucleus of Nicotiana benthamiana protoplasts.A yeast three-hybrid assay showed that PNPi interaction with NPR1 competes with the interaction between wheat NPR1 and TGA2.2.In barley transgenic lines over expressing PNPi,we observed reduced induction of multiple PR genes in the region adjacent to Pseudomonas syringaepv.tomato DC3000 infection.Based on these results,we hypothesize that PNPi has a role in manipulating wheat defense response via its interactions with NPR1.
In Arabidopsis,NPR1 is a key transcriptional co-regulator of systemic acquired resistance.Upon pathogen challenge,NPR1 translocates from the cytoplasm to the nucleus,where it interacts with TGA-bZIP transcription factors to activate the expression of several pathogenesis-related genes.In a screen of a yeast two-hybrid library from wheat leaves infected with Pucciniastriiformisf.sp.tritici,we identified a conserved rust effector that interacts with wheat NPR1 and named it Puccinia NPR1 interactor(PNPi).PNPi interacts with the NPR1/NIM1-like domain of NPR1 via its C-terminal domain.Using bimolecular fluorescence complementation assays,we detected the interaction between PNPi and wheat NPR1 in the nucleus of Nicotiana benthamiana protoplasts.A yeast three-hybrid assay showed that PNPi interaction with NPR1 competes with the interaction between wheat NPR1 and TGA2.2.In barley transgenic lines over expressing PNPi,we observed reduced induction of multiple PR genes in the region adjacent to Pseudomonas syringaepv.tomato DC3000 infection.Based on these results,we hypothesize that PNPi has a role in manipulating wheat defense response via its interactions with NPR1.
In Arabidopsis,NPR1 is a key transcriptional co-regulator of systemic acquired resistance.Upon pathogen challenge,NPR1 translocates from the cytoplasm to the nucleus,where it interacts with TGA-bZIP transcription factors to activate the expression of several pathogenesis-related genes.In a screen of a yeast two-hybrid library from wheat leaves infected with Pucciniastriiformisf.sp.tritici,we identified a conserved rust effector that interacts with wheat NPR1 and named it Puccinia NPR1 interactor(PNPi).PNPi interacts with the NPR1/NIM1-like domain of NPR1 via its C-terminal domain.Using bimolecular fluorescence complementation assays,we detected the interaction between PNPi and wheat NPR1 in the nucleus of Nicotiana benthamiana protoplasts.A yeast three-hybrid assay showed that PNPi interaction with NPR1 competes with the interaction between wheat NPR1 and TGA2.2.In barley transgenic lines over expressing PNPi,we observed reduced induction of multiple PR genes in the region adjacent to Pseudomonas syringaepv.tomato DC3000 infection.Based on these results,we hypothesize that PNPi has a role in manipulating wheat defense response via its interactions with NPR1.
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