A rice novel gene ALBL encoding a PPR protein functions in plastid gene translation
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- 英文论文题名：A rice novel gene ALBL encoding a PPR protein functions in plastid gene translation
- 论文作者：Yaozong Wu ; Qinghua Li ; Hualing Mi
- 英文论文作者：Yaozong Wu ; Qinghua Li ; Hualing Mi ; National Key Laboratory of Plant Molecular Genetics ; Institute of Plant Physiology and Ecology ; Shanghai Institutes for Biological Sciences ; Chinese Academy of Sciences
- 年：2016
- 作者机构：National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences;
- 英文论文关键词：PPR protein ; r RNA processing ; plastid rRNA ; chloroplast
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：850k
- 原文格式：D
- 会议级别：全国
摘要
Plastid gene translation is carried out in its ribosome which RNAs undergo posttranscriptional processing. Several pentatricopeptide repeat(PPR) proteins are involved in the processes. However,the mechanism underlying the regulatory mRNA processing for plastid ribosome genes remains to be elucidated. Here,we report that a rice PPR protein ALBL plays crucial role in plastid gene expression by participating in the maturation of the precursor of plastid 23S-4.5S rRNA. We isolated ALBL gene from a rice(Oryza sativa) mutant,nearly albino phenotype(albl) by map-based cloning method,and identified ALBL as a novel nucleus-encoded PPR protein with a C-terminal DYW domain,and localized to the chloroplast. Mutation of ALBL resulted in the dramatic decrease of photosynthetic proteins and abnormal chloroplasts in the seedling,plastid ribosome deficiency. albl was defected the maturation of 23S-4.5S r RNA,while significantly increased in the amount of its precursor transcripts. Based on these results,we propose that ALBL is required for plastid gene expression,participating in the maturation process of 23S-4.5S r RNA.
Plastid gene translation is carried out in its ribosome which RNAs undergo posttranscriptional processing. Several pentatricopeptide repeat(PPR) proteins are involved in the processes. However,the mechanism underlying the regulatory mRNA processing for plastid ribosome genes remains to be elucidated. Here,we report that a rice PPR protein ALBL plays crucial role in plastid gene expression by participating in the maturation of the precursor of plastid 23S-4.5S rRNA. We isolated ALBL gene from a rice(Oryza sativa) mutant,nearly albino phenotype(albl) by map-based cloning method,and identified ALBL as a novel nucleus-encoded PPR protein with a C-terminal DYW domain,and localized to the chloroplast. Mutation of ALBL resulted in the dramatic decrease of photosynthetic proteins and abnormal chloroplasts in the seedling,plastid ribosome deficiency. albl was defected the maturation of 23S-4.5S r RNA,while significantly increased in the amount of its precursor transcripts. Based on these results,we propose that ALBL is required for plastid gene expression,participating in the maturation process of 23S-4.5S r RNA.
Plastid gene translation is carried out in its ribosome which RNAs undergo posttranscriptional processing. Several pentatricopeptide repeat(PPR) proteins are involved in the processes. However,the mechanism underlying the regulatory mRNA processing for plastid ribosome genes remains to be elucidated. Here,we report that a rice PPR protein ALBL plays crucial role in plastid gene expression by participating in the maturation of the precursor of plastid 23S-4.5S rRNA. We isolated ALBL gene from a rice(Oryza sativa) mutant,nearly albino phenotype(albl) by map-based cloning method,and identified ALBL as a novel nucleus-encoded PPR protein with a C-terminal DYW domain,and localized to the chloroplast. Mutation of ALBL resulted in the dramatic decrease of photosynthetic proteins and abnormal chloroplasts in the seedling,plastid ribosome deficiency. albl was defected the maturation of 23S-4.5S r RNA,while significantly increased in the amount of its precursor transcripts. Based on these results,we propose that ALBL is required for plastid gene expression,participating in the maturation process of 23S-4.5S r RNA.
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