Molecular toxoplasmosis diagnostics:A systematic review and meta-analysis
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- 英文论文题名：Molecular toxoplasmosis diagnostics:A systematic review and meta-analysis
- 论文作者：Shengqun Deng ; Lei Yuan ; Jiarui Yao ; Shichen Jiang ; Jing Xia ; Qiang Huang ; Ling Kong ; Lijuan Zhou ; Hongjuan Peng
- 英文论文作者：Shengqun Deng ; Lei Yuan ; Jiarui Yao ; Shichen Jiang ; Jing Xia ; Qiang Huang ; Ling Kong ; Lijuan Zhou ; Hongjuan Peng ; Department of Pathogen Biology ; Guangdong Provincial Key Laboratory of Tropical Disease Research ; School of Public Health ; Southern Medical University
- 年：2016
- 作者机构：Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University;
- 英文论文关键词：Toxoplasma gondii ; toxoplasmosis ; molecular diagnostic ; diagnostic test accuracy ; polymerase chain reaction ; Loop-mediated isothermal amplification
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：R531.8
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：344k
- 原文格式：D
- 会议级别：全国
摘要
Objectives: To update the molecular toxoplasmosis diagnostics that have been developed, a systematic review and meta-analysis based on published studies was conducted to compare the diagnostic accuracy of molecular methods in the diagnosis of toxoplasmosis. Methods: Relevant original articals were searched from multiple databases, including PubM ed, Web of Science, Cochrane, Clinical Evidence, the China National Knowledge Infrastructure(CNKI) database, and the Wanfang academic journal full-text database. Studies were selected based on strict inclusion and exclusion criteria. Methodological appraisal was performed using QUADAS-2. Pooled sensitivity and specificity with their 95% confidence intervals(95% CIs) were calculated and summary receiver-operating curves generated. Statistical analysis was performed using software available on Review Manager(version 5.3, the Cochrane Collaboration, London, UK) and SPSS(version20.0, IBM, New York, USA). Results: 35 studies in all were included in the meta-analysis. PCR-based method(conventional, nested and real-time PCR) had a high degree of specificity(94.5%-100%)for detecting toxoplasmosis whatever the biologic sample is. Furthermore, for blood samples, specificity was lowest for nested PCR(69.7%, 95%CI:61.7-76.7%) and slightly higher in conventional and real-time PCR(81.6%, 95%CI:77.1-85.4% and 78.5%, 95%CI:69.3-85.6%, respectively). In addition, for amniotic fluid and cerebrospinal fluid samples, conventional PCR appears to be less sensitive than nested PCR and real-time PCR in the diagnosis of congenital and cerebral toxoplasmosis. The difference between them was statistically significant. Loop-mediated isothermal amplification was sensitive(82.8%, 95%CI:76.4-87.8%) and specific(98.3%, 95%CI:95.9-99.4%) for detection of Toxoplasma gondii DNA from blood. Other molecular diagnostics were specific(87.9–100%) as well, but the sensitivities were variable(42.9-96.6%). Conclusion: No important difference in specificity was found between the most commonly used PCRs, with most of them having high specificity. However, for biological samples from different source, sensitivities were variable. For amniotic fluid and cerebrospinal fluid samples, real-time PCR and nested PCR were more sensitive than conventional PCR in diagnosis of toxoplasmosis. But for blood samples, conventional PCR and real-time PCR were more sensitive than nested PCR. Loop-mediated isothermal amplification appear to be highly accuracy for detection of T. gondii DNA from blood. Alternative molecular diagnostics were specific as well,but sensitivities of them were erratic performances.
Objectives: To update the molecular toxoplasmosis diagnostics that have been developed, a systematic review and meta-analysis based on published studies was conducted to compare the diagnostic accuracy of molecular methods in the diagnosis of toxoplasmosis. Methods: Relevant original articals were searched from multiple databases, including PubM ed, Web of Science, Cochrane, Clinical Evidence, the China National Knowledge Infrastructure(CNKI) database, and the Wanfang academic journal full-text database. Studies were selected based on strict inclusion and exclusion criteria. Methodological appraisal was performed using QUADAS-2. Pooled sensitivity and specificity with their 95% confidence intervals(95% CIs) were calculated and summary receiver-operating curves generated. Statistical analysis was performed using software available on Review Manager(version 5.3, the Cochrane Collaboration, London, UK) and SPSS(version20.0, IBM, New York, USA). Results: 35 studies in all were included in the meta-analysis. PCR-based method(conventional, nested and real-time PCR) had a high degree of specificity(94.5%-100%)for detecting toxoplasmosis whatever the biologic sample is. Furthermore, for blood samples, specificity was lowest for nested PCR(69.7%, 95%CI:61.7-76.7%) and slightly higher in conventional and real-time PCR(81.6%, 95%CI:77.1-85.4% and 78.5%, 95%CI:69.3-85.6%, respectively). In addition, for amniotic fluid and cerebrospinal fluid samples, conventional PCR appears to be less sensitive than nested PCR and real-time PCR in the diagnosis of congenital and cerebral toxoplasmosis. The difference between them was statistically significant. Loop-mediated isothermal amplification was sensitive(82.8%, 95%CI:76.4-87.8%) and specific(98.3%, 95%CI:95.9-99.4%) for detection of Toxoplasma gondii DNA from blood. Other molecular diagnostics were specific(87.9–100%) as well, but the sensitivities were variable(42.9-96.6%). Conclusion: No important difference in specificity was found between the most commonly used PCRs, with most of them having high specificity. However, for biological samples from different source, sensitivities were variable. For amniotic fluid and cerebrospinal fluid samples, real-time PCR and nested PCR were more sensitive than conventional PCR in diagnosis of toxoplasmosis. But for blood samples, conventional PCR and real-time PCR were more sensitive than nested PCR. Loop-mediated isothermal amplification appear to be highly accuracy for detection of T. gondii DNA from blood. Alternative molecular diagnostics were specific as well,but sensitivities of them were erratic performances.
Objectives: To update the molecular toxoplasmosis diagnostics that have been developed, a systematic review and meta-analysis based on published studies was conducted to compare the diagnostic accuracy of molecular methods in the diagnosis of toxoplasmosis. Methods: Relevant original articals were searched from multiple databases, including PubM ed, Web of Science, Cochrane, Clinical Evidence, the China National Knowledge Infrastructure(CNKI) database, and the Wanfang academic journal full-text database. Studies were selected based on strict inclusion and exclusion criteria. Methodological appraisal was performed using QUADAS-2. Pooled sensitivity and specificity with their 95% confidence intervals(95% CIs) were calculated and summary receiver-operating curves generated. Statistical analysis was performed using software available on Review Manager(version 5.3, the Cochrane Collaboration, London, UK) and SPSS(version20.0, IBM, New York, USA). Results: 35 studies in all were included in the meta-analysis. PCR-based method(conventional, nested and real-time PCR) had a high degree of specificity(94.5%-100%)for detecting toxoplasmosis whatever the biologic sample is. Furthermore, for blood samples, specificity was lowest for nested PCR(69.7%, 95%CI:61.7-76.7%) and slightly higher in conventional and real-time PCR(81.6%, 95%CI:77.1-85.4% and 78.5%, 95%CI:69.3-85.6%, respectively). In addition, for amniotic fluid and cerebrospinal fluid samples, conventional PCR appears to be less sensitive than nested PCR and real-time PCR in the diagnosis of congenital and cerebral toxoplasmosis. The difference between them was statistically significant. Loop-mediated isothermal amplification was sensitive(82.8%, 95%CI:76.4-87.8%) and specific(98.3%, 95%CI:95.9-99.4%) for detection of Toxoplasma gondii DNA from blood. Other molecular diagnostics were specific(87.9–100%) as well, but the sensitivities were variable(42.9-96.6%). Conclusion: No important difference in specificity was found between the most commonly used PCRs, with most of them having high specificity. However, for biological samples from different source, sensitivities were variable. For amniotic fluid and cerebrospinal fluid samples, real-time PCR and nested PCR were more sensitive than conventional PCR in diagnosis of toxoplasmosis. But for blood samples, conventional PCR and real-time PCR were more sensitive than nested PCR. Loop-mediated isothermal amplification appear to be highly accuracy for detection of T. gondii DNA from blood. Alternative molecular diagnostics were specific as well,but sensitivities of them were erratic performances.
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