赤眼鳟IRF7基因cDNA克隆及感染GCRV后表达差异
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- 英文论文题名：Molecular Cloning and Expression Analysis of Interferon regulatory factor 7 Gene in Barbel Chub Squaliobarbus Curriculus
- 论文作者：赵鑫 ; 王荣华 ; 刘巧林 ; 王静安 ; 徐莹 ; 肖调义
- 英文论文作者：ZHAO Xin ; WANG Ronghua ; LIU Qiaolin ; WANG Jingan ; XU Ying ; XIAO Tiaoyi ; Hunan Engineering Research Center for Utilization of Characteristics of Aquatic resources ; Hunan agricultural university ; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province
- 年：2016
- 作者机构：湖南农业大学湖南省特色水产资源利用工程技术研究中心;水产高效健康生产湖南省协同创新中心;
- 论文关键词：赤眼鳟 ; 干扰素调节因子7 ; 基因表达 ; GCRV
- 英文论文关键词：Squaliobarbus curriculus ; Interferon regulatory factor 7 ; Gene Expression ; GCRV
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：561k
- 原文格式：D
- 会议级别：全国
摘要
为研究赤眼鳟(Squaliobarbus curriculus)干扰素调节因子7(Interferon regulatory factor7,IRF7)(ScIRF7)抗病毒免疫功能,采用RACE方法克隆得到ScIRF7基因cDNA全长,并通过RT-qPCR检测其组织表达规律,并感染草鱼呼肠孤病毒(Grass carp reovirus,GCRV)后脾脏、鳃及外周血淋巴细胞(peripheral blood leucocytes,PBL)中IRF7基因的表达情况。研究发现:ScIRF7基因cDNA全长为1870bp,包含5’非编码区41bp,3’非编码区560bp和开放阅读框1269bp,共编码423个氨酸。RT-qPCR结果表明IRF7在检测的组织中均有表达,其中在肝脏中表达量最高,在肠、皮肤中表达量最低;经GCRV感染刺激后,赤眼鳟脾脏、鳃组织中IRF7表达量变化趋势均为先上升后下降,都在24h达到峰值;Sc PBL中IRF7表达量在24h前并无显著变化,在24h大幅上升。研究表明:ScIRF7具有IRF家族共有的保守结构域,并且能被GCRV诱导表达,推测其在赤眼鳟抗GCRV免疫反应中发挥重要作用。
Interferon regulatory factor 7(IRF7) plays a critical part in the activation of cellular defense.To investigate the antiviral function of IRF7 of Squaliobarbus curriculus(ScIRF7), the full-length cDNA sequence of ScIRF7 was cloned and characterized with RACE technique.The complete sequence of ScIRF-7 was 1870 bp consisted of 41 bp 5'-UTR,560 bp 3'-UTR and 1269 bp ORF,which encoded 423 amino acids.Furthermore, ScIRF7 mRNA was expressed in all tested tissues and the highest-level expression was observed in liver,then in spleen and gill,while lowest in the intesine and skin.After infection with GCRV,ScIRF7 mRNA expression levels in the spleen and gill both rose in the first stage and then decreased.Moreover,in Sc PBL(peripheral blood leucocytes),IRF7 mRNA expression had no obvious change but increased at 24 h.Overall,these conclusions elucidate ScIRF7 may play a significant role in the immune responses of Squaliobarbus curriculus.
Interferon regulatory factor 7(IRF7) plays a critical part in the activation of cellular defense.To investigate the antiviral function of IRF7 of Squaliobarbus curriculus(ScIRF7), the full-length cDNA sequence of ScIRF7 was cloned and characterized with RACE technique.The complete sequence of ScIRF-7 was 1870 bp consisted of 41 bp 5'-UTR,560 bp 3'-UTR and 1269 bp ORF,which encoded 423 amino acids.Furthermore, ScIRF7 mRNA was expressed in all tested tissues and the highest-level expression was observed in liver,then in spleen and gill,while lowest in the intesine and skin.After infection with GCRV,ScIRF7 mRNA expression levels in the spleen and gill both rose in the first stage and then decreased.Moreover,in Sc PBL(peripheral blood leucocytes),IRF7 mRNA expression had no obvious change but increased at 24 h.Overall,these conclusions elucidate ScIRF7 may play a significant role in the immune responses of Squaliobarbus curriculus.
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